A low-density inoculation method for the serial subcultivation of human diploid fibroblasts: An efficient model system for the study of cellular ageing

Kaji, Koichi; Matsuo, Mitsuyoshi

doi:10.1016/0047-6374(80)90034-2

Cited by 13 publications

(3 citation statements)

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“…If cultures were continuously kept in a logarithmic growth state, there was initially enhanced proliferation activity. 53,54 This is explainable by population genetic tools. If telomeric length polymorphism at each chromosome end in a fibroblast culture is considered as an analogue to the allele frequencies at a single gene locus in a natural population of individuals, one can use the concept of genetic drift.…”

Section: Correlations Of Culture Sizes and Cumulative Population Doubmentioning

confidence: 96%

“…2,3,7,9,26,38,44,45 As shown for a great number of different fibroblast strains, there is a progressive increase in replicatively senescent cells until the culture cannot be propagated further. 2,[4][5][6][7][8][9][10][11][12][13][14][15]46,47 It has been repeatedly demonstrated that the onset of that terminal state is clearly related to the replicative age, that is, to mitotic time, of the culture and not to chronological time, [48][49][50][51] and that cell proliferation capacities are not adversely affected by growth at low cell density [52][53][54] nor by enzymatic treatment at passaging. 55 Therefore, the consumption of an intrinsically determined proliferation capacity by divisions represents the final replicative boundary which human fibroblasts cannot continuously bypass.…”

Section: Introductionmentioning

confidence: 99%

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The Concept of Telomeric Non-Reciprocal Recombination (TENOR) Applied to Human Fibroblasts Grown in Serial Cultures: Concordance with Genealogical Data

Hasenmaile

Pawelec

2005

Rejuvenation Research

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Since the discovery of the limited life span of human fibroblasts some 50 years ago, many genealogical studies have been undertaken to describe growth kinetics of fibroblasts in serial cultures by their individual division behavior. It is now accepted that proliferation capacities of human fibroblasts strongly depend on their telomere lengths and integrity. Telomeres shorten with each replication round, and there is a direct correlation between cell division capacity and telomere lengths; that is, the consumption of disposable telomeric DNA repeats during cell divisions progresses until critically short telomeres determining the replicative senescence of the cells are present. Recently, we have suggested that telomeres in fibroblasts can also become elongated during DNA replication by telomeric non-reciprocal recombination (TENOR). Here we discuss genealogical data collected over the last decades as well as more recent findings on the telomere-driven replicative senescence process, and we summarize both to give an integrated picture.

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Section: Correlations Of Culture Sizes and Cumulative Population Doubmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

The Concept of Telomeric Non-Reciprocal Recombination (TENOR) Applied to Human Fibroblasts Grown in Serial Cultures: Concordance with Genealogical Data

Hasenmaile

Pawelec

2005

Rejuvenation Research

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“…D 98 °R, a hypoxanthine guanine phosphoribosyl transferase-deficient and ouabainresistant variant of HeLa cells (20,22) was kindly provided by Dr. E. J. STANBRIDGE, Department of Microbiology, College of Medicine, University of California, Irvine, Calif. TIG-I human diploid fibroblasts (8) were obtained from Tokyo Metropolitan Institute of Gerontology, Tokyo, and were at passage 44 when used for fusion. D 98 °R (1 × l07) were fused with TIG-1 fibroblasts (5 × 106) in a 1-ml volume of suspension in the presence of polyethylene glycol (MW: 4000) (Koch-Light, LTD, Haverhill, England).…”

Section: Cell Culture and Hybridizationmentioning

confidence: 99%

Viral replication in HeLa/fibroblast hybrid cells infected with human cytomegalovirus

Tsutsui

Sonta

Kashiwai

et al. 1987

Archives of Virology

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Three human hybrid cell lines were generated by the fusion of D 98OR, a HeLa cell variant, and TIG human diploid fibroblasts. Chromosome numbers of the hybrid cells fell between that of D 98OR cells and the combined chromosome number of the two cell lines, with three marker chromosomes identical to those of D 98OR cells. One hybrid, B-3, produced large amounts of human fibronectin as analyzed by immunohistochemistry. Only human cytomegalovirus (HCMV) infected B-3 cells showed positive fluorescence as detected by human antiserum to HCMV. Further cloning of B-3 by limiting dilution resulted in two cloned hybrids with markedly enhanced virus production as compared with B-3 cells. Treatment of these two clones with phorbol ester further enhanced virus production. These cloned hybrids may provide a tool to analyze host cell factors controlling the transcription and replication of HCMV.

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Cell Culture as a Model

Cristofalo

Pignolo

1995

Comprehensive Physiology

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The sections in this article are: Historical Development of Cell Cultures for the Study of Aging Relevance of in Vitro Cellular Senescence to in Vivo Aging Cell Cultures as Models Cellular Mortality, Cellular Clocks, and Death as an End Point for Aging Aging and Evolution In Vivo–In Vitro Parallel Changes Caveats in the Use of Cell Cultures as Models Cellular and Molecular Markers of Senescence in Vitro and in Vivo Mechanistic Studies of in Vitro Replicative Senescence Stochastic Mechanisms Signal Transduction Pathways Genetic Mechanisms Summary and Conclusions

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A low-density inoculation method for the serial subcultivation of human diploid fibroblasts: An efficient model system for the study of cellular ageing

Cited by 13 publications

References 12 publications

The Concept of Telomeric Non-Reciprocal Recombination (TENOR) Applied to Human Fibroblasts Grown in Serial Cultures: Concordance with Genealogical Data

The Concept of Telomeric Non-Reciprocal Recombination (TENOR) Applied to Human Fibroblasts Grown in Serial Cultures: Concordance with Genealogical Data

Viral replication in HeLa/fibroblast hybrid cells infected with human cytomegalovirus

Cell Culture as a Model

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