A low-cost, reliable, high-throughput system for rodent behavioral phenotyping in a home cage environment

Parkison, Steven A.; Carlson, Jay D.; Chaudoin, Tammy R.; Hoke, Traci; Schenk, A. Katrin; Goulding, Evan H.; Pérez, Lance C.; Bonasera, Stephen J.

doi:10.1109/embc.2012.6346445

Cited by 11 publications

(21 citation statements)

References 19 publications

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“…Our validation tests showed that with no mice in the system, no lick events were observed for any system cage. We also noted excellent agreement in lick events determined by our lickometer compared with manual scoring of high-speed video while a mouse was licking (Parkison et al 2012). The capacitive system easily identifies missed licks (as an electrical connection was not established).…”

Section: Home Cage Monitoringsupporting

confidence: 54%

Decreased home cage movement and oromotor impairments in adult Fmr1‐KO mice

Bonasera

Chaudoin

Goulding

et al. 2017

Genes Brain and Behavior

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Fragile X syndrome (FXS) is a common inherited disorder that significantly impacts family and patient day-to-day living across the entire lifespan. The childhood and adolescent behavioral consequences of FXS are well-appreciated. However, there are significantly fewer studies (except those examining psychiatric comorbidities) assessing behavioral phenotypes seen in adults with FXS. Mice engineered with a genetic lesion of Fmr1 recapitulate important molecular and neuroanatomical characteristics of FXS, and provide a means to evaluate adult behavioral phenotypes associated with FXS. We give the first description of baseline behaviors including feeding, drinking, movement, and their circadian rhythms; all observed over 16 consecutive days following extensive environmental habituation in adult Fmr1-KO mutant mice. We find no genotypic changes in mouse food ingestion, feeding patterns, metabolism, or circadian patterns of movement, feeding, and drinking. After habituation, Fmr1-KO mice demonstrate significantly less daily movement during their active phase (the dark cycle). However, Fmr1-KO mice have more bouts of activity during the light cycle compared to wildtypes. In addition, Fmr1-KO mice demonstrate significantly less daily water ingestion during the circadian dark cycle, and this reduction in water intake is accompanied by a decrease in the amount of water ingested per lick. The observed water ingestion and circadian phenotypes noted in Fmr1-KO mice recapitulate known clinical aspects previously described in FXS. The finding of decreased movement in Fmr1-KO mice is novel, and suggests a dissociation between baseline and novelty-evoked activity for Fmr1-KO mice.

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Section: Home Cage Monitoringsupporting

confidence: 54%

Decreased home cage movement and oromotor impairments in adult Fmr1‐KO mice

Bonasera

Chaudoin

Goulding

et al. 2017

Genes Brain and Behavior

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“…Details regarding home cage monitoring (HCM) system design and data analysis algorithms have been described ( Goulding et al, 2008 ; Parkison et al, 2012 ). Briefly, mice are individually housed in low profile (48.3 cm long×25.7 cm wide×15.2 cm high, PC10196HT, Allentown) instrumented cages with ad lib access to food (while breaking a photobeam) and water (while activating a capacitive lickometer).…”

Section: Methodsmentioning

confidence: 99%

Influence of age, irradiation and humanization on NSG mouse phenotypes

et al. 2015

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Humanized mice are frequently utilized in bench to bedside therapeutic tests to combat human infectious, cancerous and degenerative diseases. For the fields of hematology-oncology, regenerative medicine, and infectious diseases, the immune deficient mice have been used commonly in basic research efforts. Obstacles in true translational efforts abound, as the relationship between mouse and human cells in disease pathogenesis and therapeutic studies requires lengthy investigations. The interplay between human immunity and mouse biology proves ever more complicated when aging, irradiation, and human immune reconstitution are considered. All can affect a range of biochemical and behavioral functions. To such ends, we show age- and irradiation-dependent influences for the development of macrocytic hyper chromic anemia, myelodysplasia, blood protein reductions and body composition changes. Humanization contributes to hematologic abnormalities. Home cage behavior revealed day and dark cycle locomotion also influenced by human cell reconstitutions. Significant age-related day-to-day variability in movement, feeding and drinking behaviors were observed. We posit that this data serves to enable researchers to better design translational studies in this rapidly emerging field of mouse humanization.

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“…We employ false discovery rate (FDR) statistics to minimize family-wise error rates; behaviors found significant in this manner are subjected to further analysis. Full details regarding our system hardware and software characteristics have been published (Goulding et al, 2008 ; Parkison et al, 2012 ; Bonasera et al, 2017 ).…”

Section: Methodsmentioning

confidence: 99%

Toll-Like Receptor 2 Is a Regulator of Circadian Active and Inactive State Consolidation in C57BL/6 Mice

DeKorver

Chaudoin

Bonasera

2017

Front. Aging Neurosci.

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Regulatory systems required to maintain behavioral arousal remain incompletely understood. We describe a previously unappreciated role that toll-like receptor 2 (Tlr2, a membrane bound pattern recognition receptor that recognizes specific bacterial, viral, and fungal peptides), contributes toward regulation of behavioral arousal. In 4–4.5 month old mice with constitutive loss of Tlr2 function (Tlr2−/− mice), we note a marked consolidation in the circadian pattern of both active and inactive states. Specifically, Tlr2−/− mice demonstrated significantly fewer but longer duration active states during the circadian dark cycle, and significantly fewer but longer duration inactive states during the circadian light cycle. Tlr2−/− mice also consumed less food and water, and moved less during the circadian light cycle. Analysis of circadian rhythms further suggested that Tlr2−/− mice demonstrated less day-to-day variability in feeding, drinking, and movement behaviors. Reevaluation of this same mouse cohort at age 8–8.5 months revealed a clear blunting of these differences. However, Tlr2−/− mice were still noted to have fewer short-duration active states during the circadian dark cycle, and continued to demonstrate significantly less day-to-day variability in feeding, drinking, and movement behaviors. These results suggest that Tlr2 function may have a role in promoting transitions between active and inactive states. Prior studies have demonstrated that Tlr2 regulates sickness behaviors including hypophagia, hyperthermia, and decreased activity. Our work suggests that Tlr2 function also evokes behavioral fragmentation, another aspect of sickness behavior and a clinically significant problem of older adults.

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A low-cost, reliable, high-throughput system for rodent behavioral phenotyping in a home cage environment

Cited by 11 publications

References 19 publications

Decreased home cage movement and oromotor impairments in adult Fmr1‐KO mice

Decreased home cage movement and oromotor impairments in adult Fmr1‐KO mice

Influence of age, irradiation and humanization on NSG mouse phenotypes

Toll-Like Receptor 2 Is a Regulator of Circadian Active and Inactive State Consolidation in C57BL/6 Mice

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