A low-cost approach for Chlorella sorokiniana production through combined use of urea, ammonia and nitrate based fertilizers

Ribeiro, Dágon Manoel; Roncaratti, Luiz F.; Possa, G.; Garcia, Lucinda Carneiro; Cançado, Letícia Jungmann; Williams, Thomas Christopher Rhys; Brasil, Bruno S. A. F.

doi:10.1016/j.biteb.2019.100354

Cited by 12 publications

(4 citation statements)

References 42 publications

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“…However, biomass productivity was reduced by half when micronutrients contained in trace elements were removed. In this case, biomass productivity in modified commercial fertilizer medium was still comparable with those obtained when Chlorella sorokiniana was cultivated in wastewater medium resulting a productivity of about 12 to 68 mg/L/d [25][26][27]. The combination between nitrate and urea was successfully maintained the growth rate and biomass productivity, compared with the combination between urea and ammonia.…”

Section: Resultssupporting

confidence: 62%

“…Meanwhile, urea is absorbed through the Active Urea Transporter (DUR3). Nitrogen assimilation in Chlorella is done through Glutamine/ Glutamate synthase (GS-GOGAT) the main route, which leads to the production of glutamine, the precursor for amino acid and protein synthesis [27]. Major observation leads to the suggestion that combined used of urea and nitrate can enhance the growth of Chlorella sp.…”

Section: Resultsmentioning

confidence: 99%

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Development of fertilizer-based medium for the growth rate improvement of Chlorella sp. in the laboratory scale

Hidayati

Anggraini

Marno

et al. 2022

IOP Conf. Ser.: Earth Environ. Sci.

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Microalgae are unicellular photosynthetic microorganisms, ubiquitous in nature which can be found in freshwater, seawater, hypersaline lakes, even in deserts and arctic ecosystems. Chlorella sp. is a eukaryotic, unicellular, photosynthetic organism with a spherical shape of about 1 to 20 μm in diameter. In this study, the combination of commonly used Walne medium and commercial fertilizers (ZA, TSP and Urea) were proposed as a low-cost approach for the cultivation of Chlorella sp. in the laboratory basis. The modified commercial fertilizer medium formulation consisted of the combination of at least 5% of Walne with different proportion of ZA, TSP and Urea. The result indicated that the combination of 5% (v/v) Walne and 95% (v/v) urea was able to retain the algal growth as compared to the combination of Urea, TSP and ZA. The obtained specific growth for 5% (v/v) Walne and 95% (v/v) urea modified medium was 0.246 d-1 and biomass productivity resulted 28 mg/L/d, which was comparable with commonly used Walne medium. Therefore, urea was primarily considered as the most suitable nitrogen source to support the growth of Chlorella sp. in the laboratory basis. Furthermore, modified commercial fertilizer medium developed in this study could potentially substitute the enriched medium used in the laboratory for the outdoor large-scale cultivation of microalgae.

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Section: Resultssupporting

confidence: 62%

Section: Resultsmentioning

confidence: 99%

Development of fertilizer-based medium for the growth rate improvement of Chlorella sp. in the laboratory scale

Hidayati

Anggraini

Marno

et al. 2022

IOP Conf. Ser.: Earth Environ. Sci.

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“…35 This study only used BG11 media as a result of its ability to support the growth of microalgae species growing in photoautotrophic mode from various natural habitats 36 and its relatively low cost. 37 One microalgal isolate was selected on the basis of noncontamination and subsequently cultivated successfully in liquid BG11. In addition, the isolate labelled UJEA_AD had large buoyant cells and formed clumps without adhering to the flask's surface.…”

Section: Results and Discussion Sample Collection Isolation And Identificationmentioning

confidence: 99%

Bioresource potential of Tetradesmus obliquusUJEA_AD: critical evaluation of biosequestration rate, biochemical and fatty acid composition in BG11 media

Ahiahonu

Anku

Roopnarain

et al. 2021

J of Chemical Tech & Biotech

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BACKGROUND: Microalgae biomass is regarded as one of the most promising renewable biomass energy resources with the potential as a sustainable biological climate change mitigation agent, raw material for commercially valuable products and fossil fuel replacement. In view of that, this study isolated, identified and evaluated the potential of an indigenous freshwater green microalga strain, Tetradesmus obliquus UJEA_AD, collected from Emmarentia Dam, Johannesburg, South Africa.RESULTS: Morphological analysis and sequencing of the rbcL biomarker aided in identification of the isolate as T. obliquus. Tetradesmus obliquus UJEA_AD was further batch-cultivated autotrophically in BG11 medium and examined for biomass accumulation, CO 2 biofixation rate, biochemical composition and fatty acid production. The results showed a maximum biomass accumulation of 2.78 ± 0.019 g L −1 with biomass productivity of 0.153 ± 0.001 g L −1 day −1 and specific growth rate of 0.13 ± 0.004 day -1 as well as CO 2 biofixation rate of 0.265 ± 0.002 gCO 2 L −1 day −1 . The biochemical data of the isolate revealed 27.17% ± 0.021% carbohydrate, 38.00% ± 0.15% protein and 31.2% ± 0.80% lipids; with primary fatty acids such as palmitic acid (34.26% ± 2.02%), linolenic acid (18.08 ± 5.29), trans-13-octadecenoic acid (12.32 ± 0.92) and phytol, 2-methylbutanoate (12.31 ± 1.02).CONCLUSION: These findings suggest that T. obliquus UJEA_AD is capable of producing bioproducts of economic value with an excellent CO 2 bio-mitigation potential when cultivated at optimum conditions in an autotrophic mode. Moreover, lipid extracted from T. obliquus UJEA_AD met the biodiesel quality standards of the USA and EU, making the isolate a promising commercial biodiesel feedstock.

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“…On the other hand, these elements are essential for microalgae production [37]. Nitrogen sources such as nitrate, nitrite and ammonia are absorbed by different pathways that converge on the GS-GOGAT cycle for the production of amino acids and ultimately other nitrogenated metabolites [38,39]. Additionally, phosphorus is absorbed as soluble phosphate, in the form of hydrogen phosphate and dihydrogen phosphate, which is further directed to the synthesis of adenosine triphosphate (ATP), nucleic acids, phospholipids and coenzymes [38].…”

Section: Effect Of Algal Cultivation On Anaerobic Pond Palm Oil Mill mentioning

confidence: 99%

Biochemical and phylogenetic characterization of the wastewater tolerant Chlamydomonas biconvexa Embrapa|LBA40 strain cultivated in palm oil mill effluent

et al. 2021

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The increasing demand for water, food and energy poses challenges for the world´s sustainability. Tropical palm oil is currently the major source of vegetable oil worldwide with a production that exceeds 55 million tons per year, while generating over 200 million tons of palm oil mill effluent (POME). It could potentially be used as a substrate for production of microalgal biomass though. In this study, the microalgal strain Chlamydomonas biconvexa Embrapa|LBA40, originally isolated from a sugarcane vinasse stabilization pond, was selected among 17 strains tested for growth in POME retrieved from anaerobic ponds of a palm oil industrial plant located within the Amazon rainforest region. During cultivation in POME, C. biconvexa Embrapa|LBA40 biomass productivity reached 190.60 mgDW • L-1 • d-1 using 15L airlift flat plate photobioreactors. Carbohydrates comprised the major fraction of algal biomass (31.96%), while the lipidic fraction reached up to 11.3% of dry mass. Reductions of 99% in ammonium and nitrite, as well as 98% reduction in phosphate present in POME were detected after 5 days of algal cultivation. This suggests that the aerobic pond stage, usually used in palm oil industrial plants to reduce POME inorganic load, could be substituted by high rate photobioreactors, significantly reducing the time and area requirements for wastewater treatment. In addition, the complete mitochondrial genome of C. biconvexa Embrapa|LBA40 strain was sequenced, revealing a compact mitogenome, with 15.98 kb in size, a total of 14 genes, of which 9 are protein coding genes. Phylogenetic analysis confirmed the strain taxonomic status within the Chlamydomonas genus, opening up opportunities for future genetic modification and molecular breeding programs in these species.

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A low-cost approach for Chlorella sorokiniana production through combined use of urea, ammonia and nitrate based fertilizers

Cited by 12 publications

References 42 publications

Development of fertilizer-based medium for the growth rate improvement of Chlorella sp. in the laboratory scale

Development of fertilizer-based medium for the growth rate improvement of Chlorella sp. in the laboratory scale

Bioresource potential of Tetradesmus obliquusUJEA_AD: critical evaluation of biosequestration rate, biochemical and fatty acid composition in BG11 media

Biochemical and phylogenetic characterization of the wastewater tolerant Chlamydomonas biconvexa Embrapa|LBA40 strain cultivated in palm oil mill effluent

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