A low-cost and miniaturized potentiostat for sensing of biomolecular species such as TNF-α by electrochemical impedance spectroscopy

Pruna, Raquel; Palacio, F.; Baraket, Abdoullatif; Zine, Nadia; Streklas, Angelos; Bausells, Joan; Errachid, Abdelhamid; López, M.

doi:10.1016/j.bios.2017.09.049

Cited by 50 publications

(36 citation statements)

References 47 publications

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“…In particular, the proposed system takes a step forward with respect to current literature [17][18][19][20][21][22][23][24][25], since it is capable of on-board building a calibration function acquiring the signal from a given set of standards at known concentrations (calibration mode); this function is subsequently applied to calculate analyte concentration of unknown samples (measurement mode). Moreover, the proposed solution relies on a Wi-Fi connection to send the preprocessed data to a cloud service for storing and sharing exploiting the remote access feature.…”

Section: Iot Architecturementioning

confidence: 99%

A Self-Calibrating IoT Portable Electrochemical Immunosensor for Serum Human Epididymis Protein 4 as a Tumor Biomarker for Ovarian Cancer

Bianchi

Mattarozzi

Giannetto

et al. 2020

Sensors

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Nowadays, analytical techniques are moving towards the development of smart biosensing strategies for the point-of-care accurate screening of disease biomarkers, such as human epididymis protein 4 (HE4), a recently discovered serum marker for early ovarian cancer diagnosis. In this context, the present work represents the first implementation of a competitive enzyme-labelled magneto-immunoassay exploiting a homemade IoT Wi-Fi cloud-based portable potentiostat for differential pulse voltammetry readout. The electrochemical device was specifically designed to be capable of autonomous calibration and data processing, switching between calibration, and measurement modes: in particular, firstly, a baseline estimation algorithm is applied for correct peak computation, then calibration function is built by interpolating data with a four-parameter logistic function. The calibration function parameters are stored on the cloud for inverse prediction to determine the concentration of unknown samples. Interpolation function calibration and concentration evaluation are performed directly on-board, thus reducing the power consumption. The analytical device was validated in human serum, demonstrating good sensing performance for analysis of HE4 with detection and quantitation limits in human serum of 3.5 and 29.2 pM, respectively, reaching the sensitivity that is required for diagnostic purposes, with high potential for applications as portable and smart diagnostic tool for point-of-care testing.

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Section: Iot Architecturementioning

confidence: 99%

A Self-Calibrating IoT Portable Electrochemical Immunosensor for Serum Human Epididymis Protein 4 as a Tumor Biomarker for Ovarian Cancer

Bianchi

Mattarozzi

Giannetto

et al. 2020

Sensors

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show abstract

“…Finally, in the HEARTEN project [60][61][62], an integrated electrochemical biosensor was developed for the detection of TNF-α and cortisol biomarkers in real saliva, as a diagnostic medium for HF application [63]. The silicon chips, that constitute the biosensor, is bonded to a standard printed circuit board using wire bonding between the pads on the chip and the printed circuit board [64].…”

Section: B Point-of-care Testing Devices Analyzing Saliva Samplesmentioning

confidence: 99%

Point-of-Care Testing Devices for Heart Failure Analyzing Blood and Saliva Samples

Tripoliti

Fotiadis

Ioannidou

et al. 2020

IEEE Rev. Biomed. Eng.

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Heart failure (HF) is the most rapidly growing cardiovascular condition with an estimated prevalence of >37.7 million individuals globally. HF is associated with increased mortality and morbidity and confers a substantial burden, in terms of cost and quality of life, for the individuals and the healthcare systems, highlighting thus the need for early and accurate diagnosis of HF. The accuracy of HF diagnosis, severity estimation and prediction of adverse events has been improved by the utilization of blood tests measuring biomarkers. The contribution of biomarkers for HF management is intensified by the fact that they can be measured in short time at the point-of-care. This is allowed by the development of portable analytical devices, commonly known as Point-of-Care Testing (POCT) devices, exploiting the advancements in the area of microfluidics and nanotechnology. The aim of this review paper is to present a review of POCT devices used for the measurement of biomarkers facilitating decision making when managing HF patients. The devices are either commercially available or in the form of prototypes under development. Both, blood and saliva samples are considered. The challenges concerning the implementation of POCT devices and the barriers for their adoption in clinical practice are discussed.

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“…An innovative multifunctional miniature sensing system was designed by Pruna et al [49] that allows the user to choose between impedimetric, amperometric, and voltammetric measurements. Two microcontrollers, PIC32 and PIC24, are used for waveform generation, data acquisition, and computer interface.…”

Section: Journal Of Sensorsmentioning

confidence: 99%

Potentiostats for Protein Biosensing: Design Considerations and Analysis on Measurement Characteristics

et al. 2019

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The demand for the development of swift, simple, and ultrasensitive biosensors has been increasing after the introduction of innovative approaches such as bioelectronics, nanotechnology, and electrochemistry. The possibility to correlate changes in electrical parameters with the concentration of protein biomarkers in biological samples is appealing to improve sensitivity, reliability, and repeatability of the biochemical assays currently available for protein investigation. Potentiostats are the required instruments to ensure the proper cell conditioning and signal processing in accurate electrochemical biosensing applications. In this light, this review is aimed at analyzing design considerations, electrical specifications, and measurement characteristics of potentiostats, specifically customized for protein detection. This review demonstrates how a proper potentiostat for protein quantification should be able to supply voltages in a range between few mV to few V, with high resolution in terms of readable current (in the order of 100 pA). To ensure a reliable quantification of clinically relevant protein concentrations (>1 ng/mL), the accuracy of the measurement (<1%) is significant and it can be ensured with proper digital-to-analog (10-16 bits) and analog-to-digital (10-24 bits) converters. Furthermore, the miniaturisation of electrochemical systems represents a key step toward portable, real-time, and fast point-of-care applications. This review is meant to serve as a guide for the design of customized potentiostats capable of a more proper and enhanced conditioning of electrochemical biosensors for protein detection.

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A low-cost and miniaturized potentiostat for sensing of biomolecular species such as TNF-α by electrochemical impedance spectroscopy

Cited by 50 publications

References 47 publications

A Self-Calibrating IoT Portable Electrochemical Immunosensor for Serum Human Epididymis Protein 4 as a Tumor Biomarker for Ovarian Cancer

A Self-Calibrating IoT Portable Electrochemical Immunosensor for Serum Human Epididymis Protein 4 as a Tumor Biomarker for Ovarian Cancer

Point-of-Care Testing Devices for Heart Failure Analyzing Blood and Saliva Samples

Potentiostats for Protein Biosensing: Design Considerations and Analysis on Measurement Characteristics

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