A Low-cost and Enzyme-free Glucose Paper Sensor

Chen, Zheyuan; Chi, Ting-Yen; Dincel, Onder; Ts, Lin; Kameoka, Jun

doi:10.1109/embc44109.2020.9176597

Cited by 3 publications

(3 citation statements)

References 10 publications

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“…The present research demonstrated a low-cost MIP lentivirus sensor that requires no biological reagent and enables virus detection in liquid and aerosol phases without bulky equipment. We previously developed glucose and perfluorooctanesulfonic acid (PFOS) conductive molecularly imprinted paper sensors [ 23 , 24 , 25 ] and expanded this approach to the detection of viruses. Lentivirus was used as a proof-of-concept test sample because it can be used in biosafety level 2 labs and is considered a safe alternative to live coronavirus.…”

Section: Introductionmentioning

confidence: 99%

Wearable Biosensor with Molecularly Imprinted Conductive Polymer Structure to Detect Lentivirus in Aerosol

Batra,

Chi,

Huang

et al. 2023

Biosensors

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The coronavirus disease (COVID-19) pandemic has increased pressure to develop low-cost, compact, user-friendly, and ubiquitous virus sensors for monitoring infection outbreaks in communities and preventing economic damage resulting from city lockdowns. As proof of concept, we developed a wearable paper-based virus sensor based on a molecular imprinting technique, using a conductive polyaniline (PANI) polymer to detect the lentivirus as a test sample. This sensor detected the lentivirus with a 4181 TU/mL detection limit in liquid and 0.33% to 2.90% detection efficiency in aerosols at distances ranging from 30 cm to 60 cm. For fabrication, a mixture of a PANI monomer solution and virus were polymerized together to form a conductive PANI sensing element on a polyethylene terephthalate (PET) paper substrate. The sensing element exhibited formation of virus recognition sites after the removal of the virus via ultrasound sonication. A dry measurement technique was established that showed aerosol virus detection by the molecularly imprinted sensors within 1.5 h of virus spraying. This was based on the mechanism via which dispensing virus droplets on the PANI sensing element induced hybridization of the virus and molecularly imprinted virus recognition templates in PANI, influencing the conductivity of the PANI film upon drying. Interestingly, the paper-based virus sensor was easily integrated with a wearable face mask for the detection of viruses in aerosols. Since the paper sensor with molecular imprinting of virus recognition sites showed excellent stability in dry conditions for long periods of time, unlike biological reagents, this wearable biosensor will offer an alternative approach to monitoring virus infections in communities.

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Section: Introductionmentioning

confidence: 99%

Wearable Biosensor with Molecularly Imprinted Conductive Polymer Structure to Detect Lentivirus in Aerosol

Batra,

Chi,

Huang

et al. 2023

Biosensors

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“…The MIP approach has shown great advantages, including simple preparation, potential reusability, and long-term stability [12]. MIP-based methods have been demonstrated as promising approaches to various chemical/bio sensing applications [13][14][15][16][17]. PdAu-polypyrrole tailored carbon fiber paper electrode has been reported to detect MMA, but it is limited by a narrow dynamic range, only from 4.01 pM to 52.5 nM [18].…”

Section: Introductionmentioning

confidence: 99%

Cancer Biomarker Methylmalonic Acid Detection by Molecularly Imprinted Polyaniline Paper Sensor

Chen

Chi

Kameoka

2022

The 2nd International Electronic Conference on Biosensors

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“…In addition, the excellent environmental durability and availability for operating in aqueous phase make PANI one of the best candidates for molecularly imprinted conductive polymers [ 44 ]. Various research of molecularly imprinted PANI (MIP-PANI) sensors for detecting chemicals [ 45 , 46 , 47 , 48 ] and proteins [ 37 , 49 ] has been reported. However, the synthesis of MIP-PANI usually relies on electrochemical polymerization, in which the substrates must be conductive materials like precious metals or graphite, potentially increasing the cost of the entire sensor.…”

Section: Introductionmentioning

confidence: 99%

Perfluorooctanesulfonic Acid Detection Using Molecularly Imprinted Polyaniline on a Paper Substrate

Chi

Chen

Kameoka

2020

Sensors

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Perfluorinated compounds like perfluorooctanesulfonic acid (PFOS) are synthetic water pollutants and have accumulated in environments for decades, causing a serious global health issue. Conventional assays rely on liquid chromatography and mass spectroscopy that are very expensive and complicated and thus limit the large-scale monitoring of PFOS in wastewater. To achieve low-cost and accurate detection of PFOS, we designed a paper-based sensor with molecularly imprinted polyaniline electrodes that have recognition sites specific to PFOS. The calibration curve of resistivity ratios as a function of PFOS concentrations has a linear range from 1 to 100 ppt with a coefficient of determination of 0.995. The estimated limit of detection is 1.02 ppt. We also investigated attenuated total reflectance Fourier-transform infrared spectroscopy (ATR-FTIR) and X-ray photoelectron spectroscopy (XPS) spectra of the surface of the polyaniline (PANI) electrodes to propose the potential recognition sites in polyaniline matrix and the detection mechanism. This electrical paper sensor with low cost and excellent sensitivity and selectivity provides the potential for large-scale monitoring of wastewater.

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A Low-cost and Enzyme-free Glucose Paper Sensor

Cited by 3 publications

References 10 publications

Wearable Biosensor with Molecularly Imprinted Conductive Polymer Structure to Detect Lentivirus in Aerosol

Wearable Biosensor with Molecularly Imprinted Conductive Polymer Structure to Detect Lentivirus in Aerosol

Cancer Biomarker Methylmalonic Acid Detection by Molecularly Imprinted Polyaniline Paper Sensor

Perfluorooctanesulfonic Acid Detection Using Molecularly Imprinted Polyaniline on a Paper Substrate

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