A loop-mediated isothermal amplification (LAMP) assay for detection of Toxoplasma gondii infection in women with spontaneous abortion

Kheirandish, Farnaz; Fallahi, Shirzad; Mahmoudvand, Hossein; Araban, Ali; Anbari, Khatereh; Rouzbahani, Arian Karimi; Akbari, Soheila

doi:10.1007/s00203-020-02081-w

Cited by 13 publications

(17 citation statements)

References 29 publications

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“…Moreover, (+)-3-carene has also been associated with conifer resistance against insects. For example, it is important for Sitka spruce ( Picea sitchensis ) resistance against the white pine weevil ( Pissodes strobi ; Robert et al, 2010 ), in P. sylvestris against the pine sawfly Diprion pini ( Pasquier-Barre et al, 2001 ), and in lodgepole pine ( Pinus contorta ) against the Douglas-fir pitch moth ( Synanthedon novaroensis ; Rocchini et al, 2000 ). Lundborg et al (2016b) also found that (+)-3-carene appears to be a feeding deterrent to the pine weevil ( H. abietis ), however, it seems to be induced by MeJA to a lesser extent in P. abies compared to P. sylvestris ( Lundborg et al, 2016a ).…”

Section: Discussionmentioning

confidence: 99%

Variation in Methyl Jasmonate-Induced Defense Among Norway Spruce Clones and Trade-Offs in Resistance Against a Fungal and an Insect Pest

et al. 2021

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An essential component of plant defense is the change that occurs from a constitutive to an induced state following damage or infection. Exogenous application of the plant hormone methyl jasmonate (MeJA) has shown great potential to be used as a defense inducer prior to pest exposure, and could be used as a plant protection measure. Here, we examined (1) the importance of MeJA-mediated induction for Norway spruce (Picea abies) resistance against damage by the pine weevil Hylobius abietis, which poses a threat to seedling survival, and infection by the spruce bark beetle-associated blue-stain fungus Endoconidiophora polonica, (2) genotypic variation in MeJA-induced defense (terpene chemistry), and (3) correlations among resistance to each pest. In a semi-field experiment, we exposed rooted-cuttings from nine different Norway spruce clones to insect damage and fungal infection separately. Plants were treated with 0, 25, or 50 mM MeJA, and planted in blocks where only pine weevils were released, or in a separate block in which plants were fungus-inoculated or not (control group). As measures of resistance, stem area debarked and fungal lesion lengths were assessed, and as a measure of defensive capacity, terpene chemistry was examined. We found that MeJA treatment increased resistance to H. abietis and E. polonica, but effects varied with clone. Norway spruce clones that exhibited high constitutive resistance did not show large changes in area debarked or lesion length when MeJA-treated, and vice versa. Moreover, insect damage negatively correlated with fungal infection. Clones receiving little pine weevil damage experienced larger lesion lengths, and vice versa, both in the constitutive and induced states. Changes in absolute terpene concentrations occurred with MeJA treatment (but not on proportional terpene concentrations), however, variation in chemistry was mostly explained by differences between clones. We conclude that MeJA can enhance protection against H. abietis and E. polonica, but the extent of protection will depend on the importance of constitutive and induced resistance for the Norway spruce clone in question. Trade-offs among resistances do not necessarily hinder the use of MeJA, as clones that are constitutively more resistant to either pest, should show greater MeJA-induced resistance against the other.

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Section: Discussionmentioning

confidence: 99%

Variation in Methyl Jasmonate-Induced Defense Among Norway Spruce Clones and Trade-Offs in Resistance Against a Fungal and an Insect Pest

et al. 2021

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“…It is worthy to point that fungal cultivar inhibition is not an outcome particular to interactions with Escovopsis , as it also results from in vitro interactions between cultivar and other filamentous fungi ( Ortiz and Orduz, 2001 ; Silva et al, 2006 ; Barcoto et al, 2017 ; do Nascimento et al, 2017 ; Bizarria et al, 2018 ). Instead of being considered as a consequence of Escovopsis pathogenicity ( Currie, 2001 ; Currie et al, 2003 ; Reynolds and Currie, 2004 ; Folgarait et al, 2011 ; Varanda-Haifig et al, 2017 ), we suggest that such outcomes also integrate the fungal cultivar responses. These fungal-fungal interactions would depend on to what extent the fungal cultivar and Escovopsis respond to and are affected by each other.…”

Section: Discussionmentioning

confidence: 91%

“…MT could reflect the effectiveness of defensive mechanisms. Such approaches could involve fungal symbiont-secreted chitinases to avoid invasive microbes ( Erthal et al, 2009 ), and laccases ( Aylward et al, 2013 ; De Fine Licht et al, 2013 ) to detoxify Escovopsis metabolites with antimicrobial properties ( Dean and Eriksson, 1994 ; Folgarait et al, 2011 ; Becker et al, 2016 ; Divya and Sadasivan, 2016 ; Boya et al, 2017 ; Dhodary et al, 2018 ; Heine et al, 2018 ). It is possible that the low laccase activity ( De Fine Licht et al, 2013 ) would render Leucoagaricus sp.…”

Section: Discussionmentioning

confidence: 99%

“…Patterns of phylogenetic congruence suggested a tripartite coevolution between the ants, the cultivated fungi, and Escovopsis ( Currie et al, 2003 ; Gerardo et al, 2006a ). Mechanisms of interaction ( Reynolds and Currie, 2004 ; Folgarait et al, 2011 ; Marfetán et al, 2015 ; Varanda-Haifig et al, 2017 ), patterns of virulence ( Currie, 2001 ; Wallace et al, 2014 ; Marfetán et al, 2015 ), patterns of specificity ( Gerardo et al, 2004 , 2006b ; Taerum et al, 2007 ; Birnbaum and Gerardo, 2016 ), and detrimental impact of infection in ant colonies ( Currie, 2001 ) have been evaluated mainly through an Escovopsis -centered perspective. Here, considering the ant colony as a host (composed by the fungus garden and the ants), we evaluate host- Escovopsis interactions in the fungiculture of higher attines.…”

Section: Introductionmentioning

confidence: 99%

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Host Susceptibility Modulates Escovopsis Pathogenic Potential in the Fungiculture of Higher Attine Ants

et al. 2021

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Health and disease emerge from intricate interactions between genotypes, phenotypes, and environmental features. The outcomes of such interactions are context-dependent, existing as a dynamic continuum ranging from benefits to damage. In host-microbial interactions, both the host and environmental conditions modulate the pathogenic potential of a microorganism. Microbial interactions are the core of the agricultural systems of ants in the subtribe Attina, which cultivate basidiomycete fungi for food. The fungiculture environment harbors a diverse microbial community, including fungi in the genus Escovopsis that has been studied as damage-causing agent. Here, we consider the ant colony as a host and investigate to what extent its health impacts the dynamics and outcomes of host-Escovopsis interactions. We found that different ant fungal cultivars vary in susceptibility to the same Escovopsis strains in plate-assays interactions. In subcolony-Escovopsis interactions, while healthy subcolonies gradually recover from infection with different concentrations of Escovopsis conidia, insecticide-treated subcolonies evidenced traits of infection and died within 7 days. The opportunistic nature of Escovopsis infections indicates that diseases in attine fungiculture are a consequence of host susceptibility, rather than the effect of a single microbial agent. By addressing the host susceptibility as a major modulator of Escovopsis pathogenesis, our findings expand the understanding of disease dynamics within attine colonies.

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“…Drosophila suzukii (Matsumura), a close relative of D. melanogaster , is a significant agricultural pest with a broad host range that can infest a large variety of small, soft-skinned fruits ( Hauser, 2011 ; Walsh et al, 2011 ; Asplen et al, 2015 ). These flies have evolved a serrated ovipositor, unique among related Drosophila , to lay eggs inside ripening fruits where larvae feed and develop ( Hickner et al, 2016 ; Cloonan et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Sex-Dependent Effects of the Microbiome on Foraging and Locomotion in Drosophila suzukii

et al. 2021

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There is growing evidence that symbiotic microbes can influence multiple nutrition-related behaviors of their hosts, including locomotion, feeding, and foraging. However, how the microbiome affects nutrition-related behavior is largely unknown. Here, we demonstrate clear sexual dimorphism in how the microbiome affects foraging behavior of a frugivorous fruit fly, Drosophila suzukii. Female flies deprived of their microbiome (axenic) were consistently less active in foraging on fruits than their conventional counterparts, even though they were more susceptible to starvation and starvation-induced locomotion was notably more elevated in axenic than conventional females. Such behavioral change was not observed in male flies. The lag of axenic female flies but not male flies to forage on fruits is associated with lower oviposition by axenic flies, and mirrored by reduced food seeking observed in virgin females when compared to mated, gravid females. In contrast to foraging intensity being highly dependent on the microbiome, conventional and axenic flies of both sexes showed relatively consistent and similar fruit preferences in foraging and oviposition, with raspberries being preferred among the fruits tested. Collectively, this work highlights a clear sex-specific effect of the microbiome on foraging and locomotion behaviors in flies, an important first step toward identifying specific mechanisms that may drive the modulation of insect behavior by interactions between the host, the microbiome, and food.

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A loop-mediated isothermal amplification (LAMP) assay for detection of Toxoplasma gondii infection in women with spontaneous abortion

Cited by 13 publications

References 29 publications

Variation in Methyl Jasmonate-Induced Defense Among Norway Spruce Clones and Trade-Offs in Resistance Against a Fungal and an Insect Pest

Variation in Methyl Jasmonate-Induced Defense Among Norway Spruce Clones and Trade-Offs in Resistance Against a Fungal and an Insect Pest

Host Susceptibility Modulates Escovopsis Pathogenic Potential in the Fungiculture of Higher Attine Ants

Sex-Dependent Effects of the Microbiome on Foraging and Locomotion in Drosophila suzukii

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