A longitudinal study of Staphylococcus aureus colonization in pigs in Ireland

Abstract

22The emergence of methicillin-resistant Staphylococcus aureus (MRSA) in livestock has 23 refocused attention on S. aureus colonization and transmission in pigs. This study 24 investigated the effect of the S. aureus colonization status of a sow on the colonization status 25 of her piglets, and whether pigs carry the same strain of S. aureus throughout production. 26 Nasal swabs were collected from the piglets of six healthy sows two days after birth and two 27 days before and two days after they were moved into each production stage. The average 28 prevalence of S. aureus colonization varied between 26% and 73%. The odds of being S. 29 aureus positive were almost 12 times higher for piglets born to nasal-positive sows than for 30 those born to nasal-negative sows, and three times higher again for piglets born to sows that 31 were both nasal-and vaginal-positive. Isolates recovered from piglets immediately after birth 32 were indistinguishable from those of the dam as determined by phenotypic and molecular 33 typing, including microarray analysis and optical mapping. All isolates belonged to clonal 34 complex 9 and the majority exhibited a novel spa type, t10449. The findings show that the S. 35 aureus colonization status of the sow influences the colonization status of her piglets in the 36 early production stages but strains carried by pigs change over time. Multiresistant S. aureus 37 was detected, in particular post-weaning. Results suggest that sow status and management 38 practices, including mixing of pigs and antimicrobial usage at weaning, should be considered 39 when implementing control measures for S. aureus on a farm. 40Word count: 249 41

A mechanistic model for spread of livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) within a pig herd

“…The association between the MRSA status of the sow and the probability of piglets testing MRSA positive have been confirmed in several studies [ 45 – 47 ]. In our model, the probability of transmission from sow to new-born piglets was based on predictions from a study, where piglets had been sampled within one hour after birth and again after one day [ 47 ].…”

Prevalence, antimicrobial resistance and staphylococcal toxin gene of blaTEM-1a-producing Staphylococcus aureus isolated from animals in Chongqing, China

“…A total of 89 S. aureus isolates were cultured from 1371 samples picked up from March 2014 to December 2017. The isolates were originated from pigs (25), cattle (6), goats (10), rabbits (16) and chicken (32). Four MRSA strains were identified from 3 pig samples and 1 chicken sample.…”

“…Dan M et al showed that a high isolation rate of S. aureus (46.0%) was obtained from cattle farms in Xinjiang Province, China [29]. A longitudinal study in Ireland indicated the average prevalence of S. aureus colonization in pigs varied between 26% and 73% [32]. The study [31] carried out in Chongqing indicated that the prevalence (46%) of goat-associated S. aureus was higher than isolation rate (11.4%) of S. aureus obtained from goats in our study.…”

Avaliação de Ibicella lutea como agente antimicrobiano frente à Staphylococcus aureus

“…são bactérias Gram positivas ubíquas, encontradas nos mais diversos sítios e hospedeiros, estão presentes tanto na microbiota animal quanto na microbiota de indivíduos saudáveis. 1,2 Apesar de serem constituintes naturais da microbiota humana, são causas comuns de infecções de pele e mucosas, sendo também responsáveis por um considerável número de infecções hospitalares com alto risco de sepse. 3,4 Somado a isso, Staphylococcus aureus é o principal representante dos microrganismos causadores de toxinfecções alimentares causadas pelo consumo de alimentos contaminados por bactérias e/ou toxinas por estas produzidas, sendo ainda um dos principais agentes causadores de mastite bovina no sul do Brasil.…”