A longitudinal study of Escherichia coli O157 in cattle of a Dutch dairy farm and in the farm environment

Schouten, J.M.; Graat, E.A.M.; Frankena, K.; Giessen, A.W. van de; Zwaluw, W K van der; Jong, M.C.M. de

doi:10.1016/j.vetmic.2005.01.026

Cited by 41 publications

(33 citation statements)

References 32 publications

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“…During this period, animals were sampled 14 times, at 4-to 10-week intervals. For details of animal population, housing, farm management, and sampling strategy see Schouten et al [16]. Herd size averaged 75 Holstein Friesian cows, of which 20 % on average were non-lactating at each sampling.…”

Section: Field Transmission Studymentioning

confidence: 99%

“…A sample was considered positive when serotyping identified the isolate as E. coli O157. Isolates were subsequently screened by polymerase chain reaction for possession of genes encoding for the most common verocytotoxins (VTI and VTII) and the eae gene [16].…”

Section: Sample Processing and Analysismentioning

confidence: 99%

“…Detailed descriptive statistics of this study can be found in Schouten et al [16]. For each sampling, 0-29 .…”

Section: Field Studymentioning

confidence: 99%

“…The majority of outbreaks are related to cattle or products of bovine origin [10,11]. Epidemiological studies have identified cattle as the main host for E. coli O157 and other VTEC [7,[12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

“…The presence of O157 VTEC in cattle and their environment has been investigated [14][15][16][17][18][19], and several studies have shown a seasonal effect in prevalence of O157 VTEC : the shedding season appears to be summer and early autumn. Duration and magnitude of E. coli O157 : H7 shedding in cattle faeces and localization of the bacterium in the gastrointestinal tract of experimentally inoculated cattle also have been studied [20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

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Transmission and quantification of verocytotoxin-producingEscherichia coliO157 in dairy cattle and calves

Schouten¹,

Graat²,

Frankena³

et al. 2008

Epidemiol. Infect.

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SUMMARYData from a field study of 14 months duration in a naturally colonized dairy herd and data from an experiment with calves were used to quantify transmission of verocytotoxin-producing Escherichia coli (VTEC O157) in cattle. For the latter, two groups of 10 calves were randomly assigned and put out in one of two pastures. From each group, five animals were experimentally inoculated with 10 9 c.f.u. O157 VTEC and, considered infectious, put back in their group. Each of the susceptible contact calves became positive within 6 days of being reunited. The estimate of the basic reproduction ratio (R 0 ) in the experiment was 7 . 3 (95 % CI 3 . 92-11 . 5), indicating that each infectious calf will infect seven other calves on average during an assumed infectious period of 28 days in a fully susceptible population. The R 0 among dairy cows appeared to be about 10 times lower (0 . 70, 95% CI 0 . 48-1 . 04). After the transmission experiment, six contact-infected animals that were shedding continuously during the experiment were housed in a tie stall during winter. After 40 days, all six tested negative for O157 VTEC. In June, after a period of 34 weeks in which the heifers remained negative, they were put out in a clean and isolated pasture to observe whether they started shedding again. On each pasture that was infected with O157 VTEC during the transmission experiment the previous summer, newly purchased susceptible calves were placed. None of the heifers or calves started shedding during 14 weeks, indicating that both the heifers and the previously contaminated pasture did not function as reservoir of O157 VTEC.

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Section: Field Transmission Studymentioning

confidence: 99%

Section: Sample Processing and Analysismentioning

confidence: 99%

“…Detailed descriptive statistics of this study can be found in Schouten et al [16]. For each sampling, 0-29 .…”

Section: Field Studymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Transmission and quantification of verocytotoxin-producingEscherichia coliO157 in dairy cattle and calves

Schouten¹,

Graat²,

Frankena³

et al. 2008

Epidemiol. Infect.

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Milk Processing and Quality Management

Tamime¹

2008

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Designations used by companies to distinguish their products are often claimed as trademarks. All brand names and product names used in this book are trade names, service marks, trademarks or registered trademarks of their respective owners. The Publisher is not associated with any product or vendor mentioned in this book. This publication is designed to provide accurate and authoritative information in regard to the subject matter covered. It is sold on the understanding that the Publisher is not engaged in rendering professional services. If professional advice or other expert assistance is required, the services of a competent professional should be sought.

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Investigation of On-Farm Transmission Routes for Contamination of Dairy Cows with Top 7 Escherichia coli O-Serogroups

et al. 2020

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A longitudinal study of Escherichia coli O157 in cattle of a Dutch dairy farm and in the farm environment

Cited by 41 publications

References 32 publications

Transmission and quantification of verocytotoxin-producingEscherichia coliO157 in dairy cattle and calves

Transmission and quantification of verocytotoxin-producingEscherichia coliO157 in dairy cattle and calves

Milk Processing and Quality Management

Investigation of On-Farm Transmission Routes for Contamination of Dairy Cows with Top 7 Escherichia coli O-Serogroups

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