A long-term stable penicillin-sensitive potentiometric biosensor with enzyme immobilized by heterobifunctional cross-linking

Thust, M.; Schöning, Michael J.; Vetter, James L.; Kordoš, P.; Lüth, H.

doi:10.1016/0003-2670(95)00619-2

Cited by 63 publications

(28 citation statements)

References 14 publications

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“…In this measuring mode, by setting the capacitance at a fixed value (e.g. the flat band capacitance), the voltage shift that results from the generated surface potential at the interface electrolyte/sensitive layer, can be directly recorded (see also [10,11]). …”

Section: Measurementsmentioning

confidence: 99%

A capacitive field-effect sensor for the direct determination of organophosphorus pesticides

Schöning

Arzdorf

Mulchandani

et al. 2003

Sensors and Actuators B: Chemical

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Section: Measurementsmentioning

confidence: 99%

A capacitive field-effect sensor for the direct determination of organophosphorus pesticides

Schöning

Arzdorf

Mulchandani

et al. 2003

Sensors and Actuators B: Chemical

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“…The Al rear contact was deposited by means of electron beam evaporation. All experimental details of the fabrication of the EIS structures are given elsewhere [6][7][8][9][10].…”

Section: Methodsmentioning

confidence: 99%

Towards a Capacitive Enzyme Sensor for Direct Determination of Organophosphorus Pesticides: Fundamental Studies and Aspects of Development

Schöning

Arzdorf

Mulchandani

et al. 2003

Sensors

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Abstract:The realisation of a miniaturised potentiometric enzyme biosensor is presented. The biosensor chip utilises the enzyme organophosphorus hydrolase (OPH) for the direct determination of pesticides. The transducer structure of the sensor chip consists of a pHsensitive capacitive electrolyte-insulator-semiconductor (EIS) structure that reacts towards pH changes caused by the OPH-catalysed hydrolysis of the organophosphate compounds. The biosensor is operated versus a conventional Ag/AgCl reference electrode. Measurements were performed in the capacitance/voltage (C/V) and constant capacitance (ConCap) mode for the two different pesticides paraoxon and parathion. For the development of this new type of biosensor, different immobilisation strategies, influence of buffer composition and concentration, transducer material, detection limit, long-term stability and selectivity have been studied.

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“…In the case of the biosensor, the immobilised enzyme catalyses the reaction of penicillin in aqueous solution to penicilloic acid and H + ions, which results in a pH change at the surface of the sensor, depending on the concentration of penicillin in the solution [12]. Detailed information of the principle of the penicillin sensor is given elsewhere [13]. ConCap measurements of the biosensor were started with pure Polymix (without penicillin); the solution was changed after each 150 seconds.…”

Section: Methodsmentioning

confidence: 99%

A (Bio-)Chemical Field-Effect Sensor with Macroporous Si as Substrate Material and a SiO2 / LPCVD-Si3N4 Double Layer as pH Transducer

Schöning

Simonis

Ruge

et al. 2002

Sensors

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Macroporous silicon has been etched from n-type Si, using a vertical etching cell where no rear side contact on the silicon wafer is necessary. The resulting macropores have been characterised by means of Scanning Electron Microscopy (SEM). After etching, SiO 2 was thermally grown on the top of the porous silicon as an insulating layer and Si 3 N 4 was deposited by means of Low Pressure Chemical Vapour Deposition (LPCVD) as transducer material to fabricate a capacitive pH sensor. In order to prepare porous biosensors, the enzyme penicillinase has been additionally immobilised inside the porous structure. Electrochemical measurements of the pH sensor and the biosensor with an Electrolyte/Insulator/Semiconductor (EIS) structure have been performed in the Capacitance/Voltage (C/V) and Constant capacitance (ConCap) mode.

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A long-term stable penicillin-sensitive potentiometric biosensor with enzyme immobilized by heterobifunctional cross-linking

Cited by 63 publications

References 14 publications

A capacitive field-effect sensor for the direct determination of organophosphorus pesticides

A capacitive field-effect sensor for the direct determination of organophosphorus pesticides

Towards a Capacitive Enzyme Sensor for Direct Determination of Organophosphorus Pesticides: Fundamental Studies and Aspects of Development

A (Bio-)Chemical Field-Effect Sensor with Macroporous Si as Substrate Material and a SiO2 / LPCVD-Si3N4 Double Layer as pH Transducer

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