A long-term stable paper-based glucose sensor using a glucose oxidase-loaded, Mn₂BPMP-conjugated nanocarrier with a smartphone readout

Abstract

A paper-type sensor system was devised using an enzyme-loaded, artificial peroxidase-conjugated nanocarrier to maintain long-term stability with smartphone readout.

Lab-on-a-Bead: Polymeric Natural Deep Eutectic Solvents as a Versatile Platform for (Bio)sensor Design

“…While the ALGPODES beads showed a decrease of 40 ± 3 % in the response (after 10 hours at room temperature), the color development decreased by 65 ± 2 % and by 67 ± 3 %, when beads composed of 5% ALG or 1% ALG were used. We hypothesize that these changes, which are somewhat in agreement with previous reports, [68][69][70] could be attributed to slight conformational changes in the enzymes, induced by the ALGPODES (or ALG) matrix, [71][72][73][74] that surrounds the proteins and (upon stabilization) creates a microenvironment that maintains their conformation and protects their active sites. 75 Considering that no color development was observed outside the beads, the release of the enzymes 76,77 or the redox dye to the solution is unlikely to be significant contributor to the decrease within the investigated timescale.…”

Green synthesis of silver nanoparticles using the extract of spent coffee used for paper-based hydrogen peroxide sensing device

“…This color change was recorded using a smartphone camera. Then, the red, green, and blue (RGB) program in the smartphone was applied to analyze the glucose concentration 25 .…”

A novel metal–organic framework of Ba–hemin with enhanced cascade activity for sensitive glucose detection

“… 4 Enzymatic methods for glucose detection, particularly GOX-based sensors, have been widely studied and extensively applied in practice, with the merits of high sensitivity, excellent selectivity and low cost. 5 However, these enzymatic methods face the challenge of the activity and stability of native enzymes being easily affected by external environmental factors (such as temperature, pH and humidity). Thus, a reliable means to maintain enzyme activity is expected to improve the performance of enzymatic sensors.…”