A long term field study of the effect of fungicides penconazole and sulfur on yeasts in the vineyard

Cordero-Bueso, Gustavo; Arroyo, Teresa; Valero, Eva

doi:10.1016/j.ijfoodmicro.2014.08.013

Cited by 26 publications

(23 citation statements)

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“…Our results pointed out that there is a greater number of species found on wildlife vines (23), compared to cultivated ones, with only seven species. This is in line with other studies, which demonstrated that the biodiversity level of yeasts community is influenced by human activities (Cordero-Bueso et al, 2011a , b , 2014 , 2017 ; Martins et al, 2014 ; Drumonde-Neves et al, 2016 ). In addition, S. cerevisae was also isolated on wildlife grape surfaces.…”

Section: Discussionsupporting

confidence: 92%

“…In general, interactions are not between two single microorganisms and the host; they also involve the native microbiota of the host and the environmental factors (i.e., the variation of the climatic conditions and other abiotic factors such as the soil, plant emplacement, or nutrient availability for the plant). In the case of the vineyards, efforts to understand the influence of different agronomic parameters on yeast populations associated to grape-berries have been published (Cordero-Bueso et al, 2011a , b , 2014 ) but there is still a lack of bibliography. Moreover, there are unexplored ecosystems such as wild vines like the protected species V. vinifera ssp sylvestris (Gmelin) Hegi which could represent a great reservoir of novel and promising yeast species to be used in the food industry, as well as a substitutive of agrochemicals.…”

Section: Introductionmentioning

confidence: 99%

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Wild Grape-Associated Yeasts as Promising Biocontrol Agents against Vitis vinifera Fungal Pathogens

et al. 2017

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The increasing level of hazardous residues in the environment and food chains has led the European Union to restrict the use of chemical fungicides. Thus, exploiting new natural antagonistic microorganisms against fungal diseases could serve the agricultural production to reduce pre- and post-harvest losses, to boost safer practices for workers and to protect the consumers' health. The main aim of this work was to evaluate the antagonistic potential of epiphytic yeasts against Botrytis cinerea, Aspergillus carbonarius, and Penicillium expansum pathogen species. In particular, yeast isolation was carried out from grape berries of Vitis vinifera ssp sylvestris populations, of the Eurasian area, and V. vinifera ssp vinifera cultivars from three different farming systems (organic, biodynamic, and conventional). Strains able to inhibit or slow the growth of pathogens were selected by in vitro and in vivo experiments. The most effective antagonist yeast strains were subsequently assayed for their capability to colonize the grape berries. Finally, possible modes of action, such as nutrients and space competition, iron depletion, cell wall degrading enzymes, diffusible and volatile antimicrobial compounds, and biofilm formation, were investigated as well. Two hundred and thirty-one yeast strains belonging to 26 different species were isolated; 20 of them, ascribed to eight species, showed antagonistic action against all molds. Yeasts isolated from V. vinifera ssp sylvestris were more effective (up to 50%) against B. cinerea rather than those isolated from V. vinifera ssp vinifera. Six strains, all isolated from wild vines, belonging to four species (Meyerozyma guilliermondii, Hanseniaspora uvarum, Hanseniaspora clermontiae, and Pichia kluyveri) revealed one or more phenotypical characteristics associated to the analyzed modes of antagonistic action.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Wild Grape-Associated Yeasts as Promising Biocontrol Agents against Vitis vinifera Fungal Pathogens

et al. 2017

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“…Finally, selection by the habitat can be exerted by agricultural practices. A few studies showed that fungicide applications can reduce the diversity and alter the composition of the foliar microbial community (Gu et al, 2010; Moulas et al, 2013; Cordero-Bueso, Arroyo & Valero, 2014; Karlsson et al, 2014). However, several other studies showed that the foliar fungal communities of grapevine are highly resilient to some chemical or biological pesticides (Walter et al, 2007; Perazzolli et al, 2014; Ottesen et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Foliar fungal communities strongly differ between habitat patches in a landscape mosaic

Fort

Robin

Capdevielle

et al. 2016

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BackgroundDispersal events between habitat patches in a landscape mosaic can structure ecological communities and influence the functioning of agrosystems. Here we investigated whether short-distance dispersal events between vineyard and forest patches shape foliar fungal communities. We hypothesized that these communities homogenize between habitats over the course of the growing season, particularly along habitat edges, because of aerial dispersal of spores.MethodsWe monitored the richness and composition of foliar and airborne fungal communities over the season, along transects perpendicular to edges between vineyard and forest patches, using Illumina sequencing of the Internal Transcribed Spacer 2 (ITS2) region.ResultsIn contrast to our expectation, foliar fungal communities in vineyards and forest patches increasingly differentiate over the growing season, even along habitat edges. Moreover, the richness of foliar fungal communities in grapevine drastically decreased over the growing season, in contrast to that of forest trees. The composition of airborne communities did not differ between habitats. The composition of oak foliar fungal communities change between forest edge and centre.DiscussionThese results suggest that dispersal events between habitat patches are not major drivers of foliar fungal communities at the landscape scale. Selective pressures exerted in each habitat by the host plant, the microclimate and the agricultural practices play a greater role, and might account for the differentiation of foliar fugal communities between habitats.

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“…Orchards are specific environments especially subjected to the successive annual application of fungicides. Many reports have shown that fungicides application may affect the biomass of non-target microorganisms (Pal et al 2008 ; Tejada et al 2011 ), change their biochemical activity (Bending et al 2007 ; Wang et al 2009a , 2016 ; Milenkovski et al 2010 ; Muñoz-Leoz et al 2011 ) as well as structural (Wang et al 2009b ; Cordero-Bueso et al 2014 ; You et al 2016 ), functional (Muñoz-Leoz et al 2011 ; Wang et al 2012 ; Wu et al 2015 ) and genetic diversity (Yen et al 2009 ; Gu et al 2010 ). Changes in microbial biota may be reflected in soil diversity, a key factor for the supplying of ecosystem goods and services to human society, which determines the ecological responses of terrestrial ecosystems to environmental change (Bardgett and van der Putten 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Non-target impact of fungicide tetraconazole on microbial communities in soils with different agricultural management

2016

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Effect of the fungicide tetraconazole on microbial community in silt loam soils from orchard with long history of triazole application and from grassland with no known history of fungicide usage was investigated. Triazole tetraconazole that had never been used on these soils before was applied at the field rate and at tenfold the FR. Response of microbial communities to tetraconazole was investigated during 28-day laboratory experiment by determination of changes in their biomass and structure (phospholipid fatty acids method—PLFA), activity (fluorescein diacetate hydrolysis—FDA) as well as changes in genetic (DGGE) and functional (Biolog) diversity. Obtained results indicated that the response of soil microorganisms to tetraconazole depended on the management of the soils. DGGE patterns revealed that both dosages of fungicide affected the structure of bacterial community and the impact on genetic diversity and richness was more prominent in orchard soil. Values of stress indices—the saturated/monounsaturated PLFAs ratio and the cyclo/monounsaturated precursors ratio, were almost twice as high and the Gram-negative/Gram-positive ratio was significantly lower in the orchard soil compared with the grassland soil. Results of principal component analysis of PLFA and Biolog profiles revealed significant impact of tetraconazole in orchard soil on day 28, whereas changes in these profiles obtained for grassland soil were insignificant or transient. Obtained results indicated that orchards soil seems to be more vulnerable to tetraconazole application compared to grassland soil. History of pesticide application and agricultural management should be taken into account in assessing of environmental impact of studied pesticides.Electronic supplementary materialThe online version of this article (doi:10.1007/s10646-016-1661-7) contains supplementary material, which is available to authorized users.

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A long term field study of the effect of fungicides penconazole and sulfur on yeasts in the vineyard

Cited by 26 publications

References 36 publications

Wild Grape-Associated Yeasts as Promising Biocontrol Agents against Vitis vinifera Fungal Pathogens

Wild Grape-Associated Yeasts as Promising Biocontrol Agents against Vitis vinifera Fungal Pathogens

Foliar fungal communities strongly differ between habitat patches in a landscape mosaic

Non-target impact of fungicide tetraconazole on microbial communities in soils with different agricultural management

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