A Localized Haemolysis in Gel Method for the Detection of Cells Producing 7S Antibody: Detection of Cells producing 7S Antibodies by the Plaque Technique

Šterzl, J.; Říha, I.

doi:10.1038/208858a0

Cited by 192 publications

(31 citation statements)

References 3 publications

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“…The following target erythrocytes were used: (a) HRBC or SRBC to detect anti-HRBC and anti-SRBC PFC, respectively; (b) F3tG -coated SRBC to detect anti-F3~G PFC according to the technique of Miller and Warner (13) ; (c) NIP azide-coated SRBC according to the technique of Pasanen and M/ikel~ (24). Guinea pig serum served as a source of complement and a rabbit anti-mouse immunoglobulin serum diluted appropriately was added to detect indirect PFC as described elsewhere (25,26).…”

Section: Antigens--fowl Immunoglobulin G (F3'g) Sheep Erythrocytes mentioning

confidence: 99%

Cell-to-Cell Interaction in the Immune Response

Miller

Sprent

Basten

et al. 1971

The Journal of Experimental Medicine

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Experiments were designed to test the possibility that thymus-derived (T) cells cooperate with nonthymus derived (B) cells in antibody responses by acting as passive carriers of antigen. Thoracic duct lymphocytes (TDL) from fowl γG-tolerant mice were incubated in vitro with fowl anti-mouse lymphocyte globulin (FALG), which was shown not to be immunosuppressive in mice. On transfer into adult thymectomized, irradiated, and marrow protected (TxBM) hosts together with a control antigen, horse RBC, a response to horse RBC but not to fowl γG was obtained. By contrast, TxBM recipients of nontolerant, FALG-coated TDL responded to both antigens and the antibody-forming cells were shown to be derived from the host, not from the injected TDL. These findings suggested that, under the conditions of the experiment, triggering of unprimed B cells in the spleens of TxBM hosts was not achieved with antigen-coated tolerant lymphocytes. Another model utilized the ability of B cells to bind antibody-antigen complexes. Spleen cells from TxBM mice, incubated in vitro with anti-fowl γG-fowl γG·NIP, were injected with or without normal TDL (a source of T cells) into irradiated hosts. Only mice given both cell types could produce an anti-NIP antibody response. In a further experiment, spleen cells from HGG·NIP-primed mice were injected together with NIP-coated B cells (prepared as above) into irradiated hosts. A substantial anti-NIP antibody response occurred. If, however, the T cells in the spleens of HGG·NIP-primed mice were eliminated by treatment with anti-θ serum and complement, the NIP response was abolished. It was concluded that antigen-coated B cells could not substitute for T cells either in the primary or secondary response. Treatment of T cells from unprimed or primed mice with mitomycin C impaired their capacity to collaborate with B cells on transfer into irradiated hosts. Taken together these findings suggest that before collaboration can take place T cells must be activated by antigen to differentiate and in so doing may produce some factor essential for triggering of B cells.

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Section: Antigens--fowl Immunoglobulin G (F3'g) Sheep Erythrocytes mentioning

confidence: 99%

Cell-to-Cell Interaction in the Immune Response

Miller

Sprent

Basten

et al. 1971

The Journal of Experimental Medicine

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“…In our earlier research, we have shown that hypoxia alone increased the immune response of adult rabbits (11 (2,4), except with undiluted guinea pig complement to increase the size and definition of the otherwise small and ill-defined plaques. After secondary immunization, the indirect PFC method was also used (9), and the hemagglutination (HA) titer of the serum also was measured.…”

mentioning

confidence: 99%

The Effect of Vitamin E on the Immune Response of Hypoxic and Normal Chickens*

Tengerdy

Heinzerling

1972

Annals of the New York Academy of Sciences

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Chicks and hens raised on a diet enriched with 60 mg of vitamin E per lb. of feed had a significantly increased immune response as measured by the antibody plaque-forming cell test or by hemagglutination. Since the effect was much greater on hypoxic (simulated altitude exposed) chicks, a synergistic effect between hypoxia and vitamin E may be suggested.Vitamin E, which was first recognized as a fertility factor for rats (10) and is known as a powerful antioxidant (7), apparently has other important but less known functions. Among these functions may be the involvement in the cellular development of the hemopoietic-immunopoietic system, the cells of which most likely originate from a common pluripotential stem cell (12). The involvement of vitamin E in the development and population kinetics of erythroid cells has already been demonstrated (3,6,8 Fig. 1 that vitamin E significantly increased the number of AB PFC both at ground level (1,500 m) and at 4,800 m simulated altitude level. However, the great increase in PFC in the altitude group indicated that the effect of vitamin E and hypoxia was synergistic.The unusually high background counts in Fig. 1 as compared to those obtained by others (1) probably were due to the use of undiluted guinea pig complement and indicated a large percentage of nonspecifically reacting cells, which are most likely not precursors of PFC (5). Those cells which responded specifically to the SRBC immunogenic stimulus, however, gave significant differences within treatment groups and compared to the controls (P < 0.05). The background PFC counts among diet and altitude controls were not significantly different. The HA titers in these young chicks at this stage were too low to interpret correctly.The chicks were reimmunized at age 31 days, partly to see whether a residual effect of the hypoxic shock remained and partly to see the change in the secondary response. Figure 2 shows the result of an indirect PFC test, showing that (i) the previous hypoxic shock had no

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“…Indirect (IgG) plaque forming cells were developed by the adaption of Sterzl & Riha (1965). The rabbit anti-IgG serum required was prepared according to Zaalberg, Van der Meul & Van Twisk (1968) and used in a dilution of 1:240.…”

Section: Direct and Indirect Plaque Forming Cellsmentioning

confidence: 99%

Histamine 2-receptor-mediated immunomodulation in the mouse. II. Immunomodulation by the H2 antagonist metiamide

Dijk¹,

Rademaker²,

Kerkhofs³

et al. 1980

International Journal of Immunopharmacology

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Abstract--Modulation of delayed hypersensitivity and antibody formation to sheep red cells by metiamide were studied in the mouse system. Depending on the time and dose of antigen and metiamide administration suppression or enhancement of the delayed hypersensitivity response was observed. The effects in this system did not differ from those reported for the H2 agonist tolazoline, which were most probably mediated by suppressor cells. As far as the humoral response was concerned metiamide tended to stimulate the IgM response. Optimal stimulation was reached if 50 mg metiamide/kg was administered 3 days before immunization with 2 × 108 SRBC. Under all conditions tested the IgG response was unaffected. These results suggest antagonistic effects of metiamide for tolazoline and adduce further evidence for the presence of H2 receptors on B ceils. The IgM production per plasmacell was enhanced suggesting different H2 receptors to be involved in differentiative and proliferative B cell responses. The possible consequences of H2 antagonist application in human therapy are discussed.

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A Localized Haemolysis in Gel Method for the Detection of Cells Producing 7S Antibody: Detection of Cells producing 7S Antibodies by the Plaque Technique

Cited by 192 publications

References 3 publications

Cell-to-Cell Interaction in the Immune Response

Cell-to-Cell Interaction in the Immune Response

The Effect of Vitamin E on the Immune Response of Hypoxic and Normal Chickens*

Histamine 2-receptor-mediated immunomodulation in the mouse. II. Immunomodulation by the H2 antagonist metiamide

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