A living cell-based biosensor utilizing G-protein coupled receptors: Principles and detection methods

Feng, Xiaojun; Castracane, James; Tokranova, Natalya; Gracias, Alison; Lnenicka, Gregory A.; Szaro, Ben G.

doi:10.1016/j.bios.2007.03.002

Cited by 21 publications

(9 citation statements)

References 28 publications

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“…Recently, development of chemical sensing elements has focused on biological molecules or receptor proteins (Feng et al, 2007;Figueroa et al, 2010;Kim et al, 2009;Saito et al, 2009;Sakata and Miyahara, 2008;Radhika et al, 2007;Vidic et al, 2008). A number of cell-based odorant sensors with high sensitivity have been studied and proposed; however, practical development has been inhibited due to shortcomings unique to cells expressing receptor proteins (Du et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Novel cell-based odorant sensor elements based on insect odorant receptors

Mitsuno

Sakurai

Namiki

et al. 2015

Biosensors and Bioelectronics

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Section: Introductionmentioning

confidence: 99%

Novel cell-based odorant sensor elements based on insect odorant receptors

Mitsuno

Sakurai

Namiki

et al. 2015

Biosensors and Bioelectronics

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“…To exploit the properties of living cells for application in chemical sensors, electrical measuring methods of cells including the patch clamp technique have been suggested (12)(13)(14). However, these methods are unsuited for a portable device because laborious cell handling using micromanipulators under a microscope and noise reduction processes are required.…”

mentioning

confidence: 99%

Highly sensitive and selective odorant sensor using living cells expressing insect olfactory receptors

Misawa

Mitsuno

Kanzaki

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

113

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This paper describes a highly sensitive and selective chemical sensor using living cells (Xenopus laevis oocytes) within a portable fluidic device. We constructed an odorant sensor whose sensitivity is a few parts per billion in solution and can simultaneously distinguish different types of chemicals that have only a slight difference in double bond isomerism or functional group such as ─OH, ─CHO and ─Cð═OÞ─. We developed a semiautomatic method to install cells to the fluidic device and achieved stable and reproducible odorant sensing. In addition, we found that the sensor worked for multiple-target chemicals and can be integrated with a robotic system without any noise reduction systems. Our developed sensor is compact and easy to replace in the system. We believe that the sensor can potentially be incorporated into a portable system for monitoring environmental and physical conditions. fluidic channel | odorant receptor | robot | Xenopus oocyte

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“…Cell immobilization can facilitate the display of a variety of targets or enable measurement of physiological responses. Amphibian tumor cells (FT cells) have been cultured on a Cr/Au microelectrode array chip [91]. Oxidation of norepinephrine (released by the cells upon stimulation with ATP via an endogenous receptor) and an increase in intracellular calcium were measured.…”

Section: Cell Immobilization and Arraysmentioning

confidence: 99%