A liver-immune coculture array for predicting systemic drug-induced skin sensitization

Chong, Lor Huai; Li, Huan; Wetzel, Isaac; Cho, Hansang; Toh, Yi‐Chin

doi:10.1039/c8lc00790j

Cited by 23 publications

(24 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The same liver-colorectal cancer coculture proved successful for the bioactivation by the liver of the pro-drug cyclophosphamide and enabled more potent suppression of the endothelial inflammatory response [36]. Finally, using a liver-immune system coculture, drug-induced skin sensitization was successfully predicted in vitro by modeling drug metabolism by 3D liver tissue and evaluating immune cascade activation by the resulting metabolites [37]. This last example further illustrates the importance of multi-OoC models for evaluating systemic drug effects that involve multiple processes and different organs.…”

Section: Drug Metabolismmentioning

confidence: 99%

“…In this final section we discuss the challenges we have identified for building multi-OoC platforms (some of which are also valid for single OoCs) regarding the specificity and constraints of each [13,18,[27][28][29][30][31][32][33][34][35]37,39,40,[43][44][45][46][47]50,51,53,54,88,93,94] can be found in the reference list at the end of the paper.…”

Section: Cancer Metastasismentioning

confidence: 99%

See 1 more Smart Citation

Multiorgan-on-a-Chip: A Systemic Approach To Model and Decipher Inter-Organ Communication

Picollet-D’hahan

Żuchowska

Lemeunier

et al. 2021

Trends in Biotechnology

202

151

View full text Add to dashboard Cite

Multiorgan-on-a-chip (multi-OoC) platforms have great potential to redefine the way in which human health research is conducted. After briefly reviewing the need for comprehensive multiorgan models with a systemic dimension, we highlight scenarios in which multiorgan models are advantageous. We next overview existing multi-OoC platforms, including integrated body-on-a-chip devices and modular approaches involving interconnected organ-specific modules. We highlight how multi-OoC models can provide unique information that is not accessible using single-OoC models. Finally, we discuss remaining challenges for the realization of multi-OoC platforms and their worldwide adoption. We anticipate that multi-OoC technology will metamorphose research in biology and medicine by providing holistic and personalized models for understanding and treating multisystem diseases.

show abstract

Section: Drug Metabolismmentioning

confidence: 99%

Section: Cancer Metastasismentioning

confidence: 99%

Multiorgan-on-a-Chip: A Systemic Approach To Model and Decipher Inter-Organ Communication

Picollet-D’hahan

Żuchowska

Lemeunier

et al. 2021

Trends in Biotechnology

202

151

View full text Add to dashboard Cite

show abstract

“…Riahi et al [58] produced microfluidic electrochemical chip immunosensors to detect the biomarkers produced during hepatotoxicity. Chong et al [59] produced assays to monitor drug skin sensitization through the assessment of metabolite production and the activation of antigen presenting cells (APCs). This system holds value as a drug screening platform to identify compounds that produce systemic skin reactions.…”

Section: Liver Ooacmentioning

confidence: 99%

Organ-on-a-chip: recent breakthroughs and future prospects

et al. 2020

View full text Add to dashboard Cite

BackgroundMicrofluidics is a science and technology that precisely manipulates and processes microscale fluids. It is commonly used to precisely control microfluidic (10 −9 to 10 −18 L) fluids using channels that range in size from tens to hundreds of microns and is known as a "lab-on-a-chip" [1][2][3][4]. The microchannel is small, but has a large surface area and high mass transfer, favoring its use in microfluidic technology applications including low regent usage, controllable volumes, fast mixing speeds, rapid responses, and precision control of physical and chemical properties [1,5,6]. Microfluidics integrate sample preparation, reactions, separation, detection, and basic operating units such as cell culture, sorting and cell lysis [7]. For these reasons, interest in OOAC has intensified [8]. OOAC combines a range of chemical, biological and material science disciplines [9] and was selected as one of the "Top Ten Emerging Technologies" in the World Economic Forum [10].OOAC is a biomimetic system that can mimic the environment of a physiological organ, with the ability to regulate key parameters including AbstractThe organ-on-a-chip (OOAC) is in the list of top 10 emerging technologies and refers to a physiological organ biomimetic system built on a microfluidic chip. Through a combination of cell biology, engineering, and biomaterial technology, the microenvironment of the chip simulates that of the organ in terms of tissue interfaces and mechanical stimulation. This reflects the structural and functional characteristics of human tissue and can predict response to an array of stimuli including drug responses and environmental effects. OOAC has broad applications in precision medicine and biological defense strategies. Here, we introduce the concepts of OOAC and review its application to the construction of physiological models, drug development, and toxicology from the perspective of different organs. We further discuss existing challenges and provide future perspectives for its application.

show abstract

“…A liver-immune co-culture array was used to study skin sensitization. 124 Hepatocyte spheroids and U937 myeloid cells were co-cultured in concentric micro-chambers, connected by a microchannel network. Model drugs known to cause cutaneous reactions (carbamazepine, phenytoin, and allopurinol) were metabolized by the liver spheroids and activate U937 cells in the immune compartment.…”

Section: Recent Advances In Multi-organ Modelsmentioning

confidence: 99%

“…Several primitive BOC systems with a relatively simple design having two or three organ modules have been reported, which may potentially lead to high-throughput platforms in the future. A liver-immune coculture array was used to study skin sensitization . Hepatocyte spheroids and U937 myeloid cells were cocultured in concentric microchambers, connected by a microchannel network.…”

Section: Recent Advances In Multiorgan Modelsmentioning

confidence: 99%

Recent Advances in Body-on-a-Chip Systems

et al. 2018

View full text Add to dashboard Cite

show abstract

A liver-immune coculture array for predicting systemic drug-induced skin sensitization

Abstract: Recapitulation of liver-immune interactions in a microfluidic compartmentalized coculture array is sufficient to accurately predict systemic drug-induced skin sensitization.

Cited by 23 publications

References 38 publications

Multiorgan-on-a-Chip: A Systemic Approach To Model and Decipher Inter-Organ Communication

Multiorgan-on-a-Chip: A Systemic Approach To Model and Decipher Inter-Organ Communication

Organ-on-a-chip: recent breakthroughs and future prospects

Recent Advances in Body-on-a-Chip Systems

Contact Info

Product

Resources

About