A liquid-phase ELISA and its use in the identification of epitopes on foot-and-mouth disease virus antigens

McCullough, Kenneth C.; Crowther, John R.; Butcher, R.N.

doi:10.1016/0166-0934(85)90026-6

Cited by 30 publications

(11 citation statements)

References 16 publications

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“…This ELISA was used for characterization of Indian isolates of type O [48] and Asia 1 [41] FMD virus. Liquid phase blocking ELISA using FMDV specific sera for characterization of field isolates was used and results were well correlated with conventional virus neutralization test [40]. Nucleic acid based diagnostic methods like PCR, multiplex PCR and real time PCR have been used for FMDV serotyping with improved sensitivity and specificity [65,72].…”

Section: Diagnosis Of Fmdmentioning

confidence: 99%

Foot-and-mouth Disease: Global Status and Future Road Map for Control and Prevention in India

et al. 2012

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Foot-and-mouth disease (FMD) is a transboundary, economically devastating and highly contagious viral disease of livestock, most importantly cattle, buffalo and pig. The disease also affects goats, sheep, wild ruminant species and elephants. The causative FMD virus is antigenically diverse having seven distinct serotypes and many variants within them. Being a single stranded RNA virus, it confirms the quasispecies nature with emergences and reemergences of different genetic lineages with altered antigenicity within the serotypes, making vaccination based control programme a high cost effective, time consuming and difficult to achieve. As per the OIE and FAO, the disease is a major threat to food security of the world, and particularly the countries having the disease are more prone to food insecurity. Further, FMD free status is an indicator of development, and all developed countries are free from it. The disease is endemic in India and three serotypes of the virus viz; O, A and Asia1 are circulating. Annual direct loss due to FMD in India has been estimated at Rs. 20,000 crores. Many countries in the world are now free from FMD with or without vaccination and presence of the disease in other neighboring countries is a major threat to them. Countries having FMD face trade barrier posed by FMD free countries, causing heavy economic loss to the livestock industry. Progressive control pathway has been developed by FAO for global eradication of FMD. Vaccination based FMD control programme is in operation in India which involves biannual vaccinations of all cattle and buffaloes in selected areas, regular active surveillance and antibody monitoring in vaccinated population with the objective of creating FMD free zones. At present, the disease occurrence, severity of the clinical disease and number of outbreaks have progressively and substantially declined in the control zones as a result of last 10 rounds of vaccination with an oil adjuvanted trivalent inactivated vaccine. In this review, FMD scenario in India and in the world is briefed. Besides, the measures taken for the control and eradication of this devastating disease is presented. Besides, the initial success achieved through the FMD control programme in India, a road map for the control and eradication of FMD at national level is discussed.

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Section: Diagnosis Of Fmdmentioning

confidence: 99%

Foot-and-mouth Disease: Global Status and Future Road Map for Control and Prevention in India

et al. 2012

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“…We also vaccinated 62 goats aged 1 to 2 years with 2 ml and 60 crossbreed cattle with 4 ml of double emulsion quadrivalent vaccine. The immune responses elicited by the quadrivalent vaccine were studied by using a virus microneutralization test (28) and a liquid-phase sandwich (blocking) enzyme-linked immunosorbent assay (ELISA) (17).…”

mentioning

confidence: 99%

Immune Responses of Sheep to Quadrivalent Double Emulsion Foot-and-Mouth Disease Vaccines: Rate of Development of Immunity and Variations among Other Ruminants

et al. 2002

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“…Therefore, our study used one (CGPYAGPLERQKPLK) of the 3B peptides (20) as the immunogen for MAb production, and the MAb produced against this peptide was used as the basis for the 3B cELISA to differentiate vaccinated from infected animals. The MAbs that are raised by peptides may not recognize original or recombinant protein (33,34). However, the MAb F8-3Bp that was produced in the current study reacted with the 3B peptide and recombinant 3ABC.…”

Section: Discussionmentioning

confidence: 85%

Development of a Competitive Enzyme-Linked Immunosorbent Assay for Detection of Antibodies against the 3B Protein of Foot-and-Mouth Disease Virus

Yang¹,

Parida²,

Salo³

et al. 2015

Clin. Vaccine Immunol.

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Foot-and-mouth disease (FMD) is one of the most highly contagious and economically devastating diseases, and it severely constrains the international trade of animals. Vaccination against FMD is a key element in the control of FMD. However, vaccination of susceptible animals raises critical issues, such as the differentiation of infected animals from vaccinated animals. The current study developed a reliable and rapid test to detect antibodies against the conserved, nonstructural proteins (NSPs) of the FMD virus (FMDV) to distinguish infected animals from vaccinated animals. A monoclonal antibody (MAb) against the FMDV NSP 3B was produced. A competitive enzyme-linked immunosorbent assay (cELISA) for FMDV/NSP antibody detection was developed using a recombinant 3ABC protein as the antigen and the 3B-specific MAb. Sera collected from naive, FMDV experimentally infected, vaccinated carrier, and noncarrier animals were tested using the 3B cELISA. The diagnostic specificity was 99.4% for naive animals (cattle, pigs, and sheep) and 99.7% for vaccinated noncarrier animals. The diagnostic sensitivity was 100% for experimentally inoculated animals and 64% for vaccinated carrier animals. The performance of this 3B cELISA was compared to that of four commercial ELISA kits using a panel of serum samples established by the World Reference Laboratory for FMD at The Pirbright Institute, Pirbright, United Kingdom. The diagnostic sensitivity of the 3B cELISA for the panel of FMDV/NSP-positive bovine serum samples was 94%, which was comparable to or better than that of the commercially available NSP antibody detection kits. This 3B cELISA is a simple, reliable test to detect antibodies against FMDV nonstructural proteins.

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A liquid-phase ELISA and its use in the identification of epitopes on foot-and-mouth disease virus antigens

Cited by 30 publications

References 16 publications

Foot-and-mouth Disease: Global Status and Future Road Map for Control and Prevention in India

Foot-and-mouth Disease: Global Status and Future Road Map for Control and Prevention in India

Immune Responses of Sheep to Quadrivalent Double Emulsion Foot-and-Mouth Disease Vaccines: Rate of Development of Immunity and Variations among Other Ruminants

Development of a Competitive Enzyme-Linked Immunosorbent Assay for Detection of Antibodies against the 3B Protein of Foot-and-Mouth Disease Virus

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