A liquid chromatography–tandem mass spectrometry method to simultaneously determinate dichlorvos and phoxim in tobacco

Abstract

A simple pretreatment method with liquid chromatography–tandem mass spectrometry (LC–MS/MS) was developed and validated to simultaneously determine dichlorvos and phoxim in tobacco and soil matrices. Satisfactory linearity (R2 ≥ 0.9991) of the method was obtained for both analytes. The limits of detection and limits of quantification for dichlorvos and phoxim in three matrices were 0.0015–0.006 and 0.005–0.02 mg/kg, respectively. Average recoveries were 78.24–92.21% for dichlorvos and 76.62–100.51% for phoxim in soil, green tobacco leaves and cured tobacco leaves. The intra‐ and inter‐day relative standard deviations were <6%. The established method was successfully applied for the residual analysis of dichlorvos and phoxim in real soil and tobacco samples. The results indicated that the established method could be used to detect trace amounts of dichlorvos and phoxim in tobacco. The data could also help the Chinese government establish maximum residue limits of dichlorvos and phoxim on tobacco and establish proper and safe use of dichlorvos and phoxim on tobacco plants in China.

Simultaneous determination of 19 constituents in Cimicifugae Rhizoma by HPLC–DAD and screening for antioxidants through DPPH free radical scavenging assay

“…The contents of the 19 investigated components in 16 batches of samples collected from different areas are summarized in Figure . It was clear that the content of analysis compounds varied significantly among different samples, which might be due to different origins (Jiang, Kronenberg, Balick, & Kennelly, ), climatic conditions (Sleifer, Schwanz, Danielli, Apel, & Henriques, ), harvest times and processing methods (Qin et al, ).…”

Analytical methods to analyze pesticides and herbicides

“…Dichlorvos and phoxim were analyzed with LC-MS/ MS (Qin et al, 2019). Satisfactory linearity (R 2 ≥ 0.9991) was observed for both target compounds.…”

Copper Ion-Induced Self-Assembled Aerogels of Carbon Dots as Peroxidase-Mimicking Nanozymes for Colorimetric Biosensing of Organophosphorus Pesticide

“…Environmental safety has become an important issue for public health and ecosystem vitality due to the harmful and detrimental consequences brought about by various pollutants emission to the environment. , Among various pollutants, organophosphorus pesticides (OPs) are a class of widely used lethal nerve agents, which can protect crops from pests and diseases in agricultural production. , However, the overuse of organophosphorus pesticides leads to high residues existing widely in food, water, and the environment. , These residual organophosphorus pesticides can irreversibly combine with the enzymes' active site of acetylcholinesterase (AChE) in living organisms to cause the buildup of neurotransmitter acetylcholine (ACh), which further poses a serious threat to human health. , Therefore, a simple, rapid, sensitive, and selective method for OP detection has become increasingly necessary. At present, there are many methods for OP detection, such as high-performance liquid chromatography, , electrochemistry, , and fluorescence. − Xu et al reported the use of high-performance liquid chromatography with an ultraviolet detector for the determination of trace dichlorvos residues in foods . Qin et al developed and validated a liquid chromatography-tandem mass spectrometry to determine dichlorvos in tobacco .…”

“…3,4 Therefore, a simple, rapid, sensitive, and selective method for OP detection has become increasingly necessary. At present, there are many methods for OP detection, such as high-performance liquid chromatography, 5,6 electrochemistry, 7,8 and fluorescence. 9−11 Xu et al reported the use of high-performance liquid chromatography with an ultraviolet detector for the determination of trace dichlorvos residues in foods.…”