A Liquid Chromatography–Tandem Mass Spectrometry Method for Salivary Testosterone with Adult Male Reference Interval Determination

Macdonald, Philip R.; Owen, Laura; Wu, Frederick C. W.; Macdowall, Wendy; Keevil, Brian

doi:10.1373/clinchem.2010.154484

Cited by 27 publications

(13 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The protocol was optimized to capture free T87 and C88 and to detect the typically low T concentrations observed in women47. Previously reported mean intra-assay coefficients of variation (CV) were 5.3% for T and 8.7% for C, whereas mean inter-assay CV were 9% for T and 7.8% for C4788.…”

Section: Methodsmentioning

confidence: 99%

Endogenous testosterone is associated with lower amygdala reactivity to angry faces and reduced aggressive behavior in healthy young women

Buades-Rotger

Engelke

Beyer

et al. 2016

Sci Rep

View full text Add to dashboard Cite

Testosterone and cortisol have been proposed to influence aggressive behavior by altering the neural processing of facial threat signals. However, this has not been investigated in direct social interactions. Here, we explored the joint impact of testosterone, cortisol, and brain reactivity to anger expressions on women’s reactive aggression in the Social Threat Aggression Paradigm (STAP). The STAP is a competitive reaction time task in which the purported opponent displays either an angry or a neutral facial expression at the beginning of each trial and delivers increasingly loud sound blasts to the participants, successfully provoking them. Strikingly, salivary testosterone at scan-time was negatively related to both aggression and basolateral amygdala (BLA) reactivity to angry faces, whereas cortisol had no effect. When the opponent looked angry, BLA-orbitofrontal coupling was reduced, and BLA reactivity was positively related to aggression. The latter relationship was fully mediated by bilateral superior temporal gyrus (STG) activation. Our results thus support previous neurobiological models of aggression, and extend them by demonstrating that fast amygdala responses to threat modulate STG activity in order to favor aggressive retaliation. Furthermore, our study agrees with recent evidence underscoring a fear-reducing and strategically prosocial effect of testosterone on human social behavior.

show abstract

Section: Methodsmentioning

confidence: 99%

Endogenous testosterone is associated with lower amygdala reactivity to angry faces and reduced aggressive behavior in healthy young women

Buades-Rotger

Engelke

Beyer

et al. 2016

Sci Rep

View full text Add to dashboard Cite

show abstract

“…In contrast to the collection of serum, which is invasive and expensive, collection of saliva is relatively straightforward and requires minimal training. We have recently demonstrated that Sal-T can be reliably and accurately measured by a highly sensitive and specific liquid chromatography–tandem mass spectrometry method (16). In a validation study comparing samples from the same individuals, we found that Sal-T in adult men and women correlated more strongly with calculated serum free-T than serum total-T (17) and was also unaffected by variations in sex hormone-binding globulin (SHBG) (18).…”

mentioning

confidence: 99%

Salivary Testosterone Levels and Health Status in Men and Women in the British General Population: Findings from the Third National Survey of Sexual Attitudes and Lifestyles (Natsal-3)

Clifton

Macdowall

Copas

et al. 2016

The Journal of Clinical Endocrinology &Amp; Metabolism

View full text Add to dashboard Cite

Context:Salivary T (Sal-T) measurement by liquid chromatography–tandem mass spectroscopy resents the opportunity to examine health correlates of Sal-T in a large-scale population survey.Objective:This study sought to examine associations between Sal-T and health-related factors in men and women age 18–74 years.Design and Setting:Morning saliva samples were obtained from participants in a cross-sectional probability-sample survey of the general British population (Natsal-3). Self-reported health and lifestyle questions were administered as part of a wider sexual health interview.Participants:Study participants included 1599 men and 2123 women.Methods:Sal-T was measured using liquid chromatography–tandem mass spectroscopy. Linear regression was used to examine associations between health factors and mean Sal-T.Results:In men, mean Sal-T was associated with a range of health factors after age adjustment, and showed a strong independent negative association with body mass index (BMI) in multivariable analysis. Men reporting cardiovascular disease or currently taking medication for depression had lower age-adjusted Sal-T, although there was no association with cardiovascular disease after adjustment for BMI. The decline in Sal-T with increasing age remained after adjustment for health-related factors. In women, Sal-T declined with increasing age; however, there were no age-independent associations with health-related factors or specific heath conditions with the exception of higher Sal-T in smokers.Conclusions:Sal-T levels were associated, independently of age, with a range of self-reported health markers, particularly BMI, in men but not women. The findings support the view that there is an age-related decline in Sal-T in men and women, which cannot be explained by an increase in ill health. Our results demonstrate the potential of Sal-T as a convenient measure of tissue androgen exposure for population research.

show abstract

“…15,20,26 Another major concern in our study is the absence of CFT validation compared to the AFTC determinated by ED in our population. 28 Our established reference values in healthy young men are a basic diagnostic tool for the suitable study of hypogonadism in our surroundings and are also useful for clinics in the Mediterranean region whose laboratories employ the same methods for determining TT and SHBG. 19 However, as we report, our method of TT quantification is very reliable, and the CFT and the AFTC were almost identical in studies of populations with SHBG capacities varying from low to extremely high, as in hyperthyroidism.…”

Section: Discussionmentioning

confidence: 99%

Reference ranges for serum and salivary testosterone in young men of Mediterranean region

González-Sánchez

Moreno-Pérez

Guadiana

et al. 2015

Endocrinología y Nutrición (English Edition)

View full text Add to dashboard Cite

Background and aims: The interassay variability found in the measurement of testosterone (T) levels warrants the need for laboratories to validate their methods to establish trustworthy cut-off points for diagnosis of male hypogonadism. The aims of this study were to validate measurement of total T (TT) at our laboratory in order to obtain reference ranges for TT, calculated free T (CFT), calculated bioavailable T (CBT), and salivary T (ST) in healthy young men from the Mediterranean region, and to evaluate the potential clinical value of ST by establishing its correlation with serum T. Methods: An observational, cross-sectional study with sequential sampling. Inclusion criteria: men aged 18---30 years with body mass index (BMI) < 30. Exclusion criteria: chronic diseases, hepatic insufficiency or use of drugs altering circulating T levels. Main outcome measures TT (chemiluminescent immunoassay UniCell DXI 800 [Access T Beckman Coulter]), CFT and CBT (Vermeulen's formula), and ST (radioimmunoassay for serum TT modified for saliva [Coat-A-Count, Siemens]). Descriptive statistical analyses and correlation by Spearman's rho (SPSS 19.0 Inc., Chicago) were used. Results: One hundred and twenty-one subjects aged 24 ± 3.6 years with BMI 24 ± 2.5 kg/m 2 were enrolled. Hormone study: TT, 19 ± 5.5 nmol/L (reference range [rr.] 9.7---33.3); CFT, 0.38 nmol/L (rr. 0.22---0.79); CBT, 9.7 nmol/L (rr. 4.9---19.2); and ST, 0.35 nmol/L (rr. 0.19---0.68). Correlation between ST and CFT was 0.46. Testosterona; Valores de referencia; Área mediterránea; Hipogonadismo masculino Valores de referencia de testosterona sérica y salivar en varones jóvenes del área Mediterránea ResumenAntecedentes y objetivos: La variabilidad interensayo existente en la determinación de testosterona (T) justifica la necesidad de que cada laboratorio valide su método y establezca puntos de corte fiables para el diagnóstico del hipogonadismo masculino. Los objetivos del estudio fueron validar la determinación de T total (TT) en nuestro laboratorio para obtener los valores de referencia de TT, T libre calculada (TLC), T biodisponible calculada (TBC) y T salivar (TS) en varones jóvenes sanos del área mediterránea y evaluar la posible utilidad clínica de la TS. Material y métodos: Estudio observacional transversal. Muestreo secuencial. Criterios de inclusión: varones, 18---30 años de edad, índice de masa corporal (IMC) < 30. Criterios de exclusión: enfermedades crónicas, insuficiencia hepática o uso de medicamentos que alteran las concentraciones circulantes de T. Metodología: TT (inmunoanálisis de quimioluminiscencia UniCell DXI800 [Acces T Beckman Coulter]), TLC y TBC (fórmula de Vermeulen), TS (radioinmunoensayo para TT sérica modificado para la saliva [Coat-A-Count, Siemens]). Análisis estadísticos: descriptivos y correlación rho de Spearman (SPSS 19,0 Inc.,Chicago). Resultados: Se incluyeron 121 individuos de 24 ± 3,6 años e IMC 24 ± 2,5 kg/m 2 . Estudio hormonal TT, 19 ± 5,5 nmol/L (intervalo de confianza 95% 9,7---33,3); TLC, 0,38 nmol/L ([P 2,5 ---P 97,5 ]...

show abstract

A Liquid Chromatography–Tandem Mass Spectrometry Method for Salivary Testosterone with Adult Male Reference Interval Determination

Cited by 27 publications

References 2 publications

Endogenous testosterone is associated with lower amygdala reactivity to angry faces and reduced aggressive behavior in healthy young women

Endogenous testosterone is associated with lower amygdala reactivity to angry faces and reduced aggressive behavior in healthy young women

Salivary Testosterone Levels and Health Status in Men and Women in the British General Population: Findings from the Third National Survey of Sexual Attitudes and Lifestyles (Natsal-3)

Reference ranges for serum and salivary testosterone in young men of Mediterranean region

Contact Info

Product

Resources

About