A liquid chromatographic method, with fluorometric detection, for the determination of enrofloxacin and ciprofloxacin in plasma and endometrial tissue of mares

González, Claudia; Moreno, Laura; Small, J.; Jones, Douglas; Bruni, Sergio Fabian Sanchez

doi:10.1016/j.aca.2005.12.040

Cited by 42 publications

(27 citation statements)

References 25 publications

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“…0.04 mg/mL obtained by Manceau et al (1999), 0.06 mg/mL reported by Knoll et al (1999) and 0.05 mg/mL acquired by Gonzalez et al (2006)]. 0.04 mg/mL obtained by Manceau et al (1999), 0.06 mg/mL reported by Knoll et al (1999) and 0.05 mg/mL acquired by Gonzalez et al (2006)].…”

Section: Resultsmentioning

confidence: 87%

“…The mean (± SD) plasma concentration-time profile are presented in Figure 2 and the pharmacokinetic parameters in Table 3. The method was used in pharmacokinetic studies -the advantage was the small volume (0.25 mL) of the sample in comparison with other methods utilizing usually 1 mL of plasma (Gonzalez et al, 2006). However, most of the described methods are quite complicated, expensive and time-consuming as they use procedures like solid phase extraction, evaporation under nitrogen stream (Gonzalez et al, 2006) or molecularly imprinted polymers (Sun et al, 2008).…”

Section: Resultsmentioning

confidence: 99%

“…Enrofloxacin is widely used in veterinary medicine and thus there are many developed methods of its determination both in plasma and tissues of many animal species. By many methods ciprofloxacin (enrofloxacin metabolite) was also determined (Manceau et al, 1999;Gonzalez et al, 2006). Our method was much simpler and consisted only of protein precipitation and dichloroethane extraction (sample evaporation was omitted).…”

Section: Resultsmentioning

confidence: 99%

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Determination of enrofloxacin in chicken plasma by high performance liquid chromatography for pharmacokinetic studies

Jakubowski¹,

Jaroszewski²,

Grabowski³

et al. 2010

Acta vet. (Beogr.)

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A simple rapid liquid chromatography method for determination of enrofloxacin in chicken plasma was developed. Samples were prepared by protein precipitation with acetonitrile/ammonia mixture and dichloroethane extraction. Analytical separation was performed in a gradient grade on a Phenomenex Gemini C18 column and analytes were detected by fluorescence (λex= 300 nm and λem= 448 nm). The acidified mobile phase (pH = 3.8) was a mixture of acetonitrile and 0.05 M acetate buffer. The method was linear in the range 0.2-3.5 μg/mL, limit of detection was 40 ng/mL and limit of quantification was 120 ng/mL. The assay was accurate and precise and the mean recovery of enrofloxacin was 90.07±0.89 %. The suitability of the assay for pharmacokinetic studies was determined by measuring enrofloxacin concentrations in chicken plasma after a single intravenous administration of the drug at a dose of 10 mg/kg. Enrofloxacin concentration decreased from 4.17 μg/mL in 5 min to 0.32 μg/mL 24 hours after drug administration. The obtained results confirmed the suitability of the method for enrofloxacin determination

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Section: Resultsmentioning

confidence: 87%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Determination of enrofloxacin in chicken plasma by high performance liquid chromatography for pharmacokinetic studies

Jakubowski¹,

Jaroszewski²,

Grabowski³

et al. 2010

Acta vet. (Beogr.)

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“…A series of analytical methods for simultaneous determination of ENFLX and CPFLX in different matrix were reported, including spectrophotometry (Mostafa et al 2002), capillary electrophoresis (CE; Barrón et al 2001;Hernandez et al 2002), CE-electrochemiluminesce (Zhou et al 2008) and liquid chromatograph (LC; Manceau et al 1999;Olutosin et al 2004;Sunderland et al 2004;Cinquina et al 2003;González et al 2006) as well as turbulent flow chromatography-tandem mass spectrometry (Krebber et al 2009). For the determination of MG in fish farming water, a spectrophtometric method was reported (Pourreza and Elhami 2007).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of malachite green, enrofloxacin and ciprofloxacin in fish farming water and fish feed by liquid chromatography with solid-phase extraction

Sun

Wang

Qin

et al. 2010

Environ Monit Assess

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An effective and sensitive method for simultaneous analysis of malachite green (MG), enrofloxacin (ENFLX) and ciprofloxacin (CPFLX) by liquid chromatography-diode array detection with solid-phase extraction (SPE) is developed. The conditions of SPE and LC were investigated and optimised. The effective separation of these compounds was achieved using a ZY1104 C18 column (250 × 4.6 mm, 5 μm) with 20 mM tetrabutyl ammonium bromide (pH 3.0)-acetonitrile as mobile phase and gradient elution. The diode array detection was used at 278 nm for ENFLX and CPFLX and at 613 nm for MG. Under the optimal conditions, the method LOD values of MG, ENFLX and CPFLX were 0.01, 0.07 and 0.10 μg L( -1) for fish farming water samples and 1.5, 10.5 and 15 μg kg( -1) for fish feed samples, respectively. The relative recoveries of the three analytes were achieved to be 76.7-82.3% with the RSDs (n = 5) of 3.2-4.6% for spiked fish farming water samples and 78.8-93.7% with the RSDs (n = 5) of 3.1-4.8% for spiked fish feed samples.

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“…According to literatures, the widely-used method for the determination of ciprofloxacin is high performance liquid chromatography (HPLC) [1][2][3][4][5] since it possesses high sensitivity and excellent selectivity. Additionally, many other methods such as spectrophotometry, 6,7 capillary zone electrophoresis, 8 micellar liquid chromatography, 9 chemiluminescence 9,10 and biosensor 12 were also reported for the analysis of ciprofloxacin.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Determination of Ciprofloxacin Based on the Enhancement Effect of Sodium Dodecyl Benzene Sulfonate

Zhang¹,

Wei²

2007

Bulletin of the Korean Chemical Society

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Herein, a new electrochemical method was described for the determination of ciprofloxacin based on the enhancement effect of an anionic surfactant: sodium dodecyl benzene sulfonate (SDBS). In pH 4.0 phosphate buffer and in the presence of 1.0 × 10 −4 mol/L SDBS, ciprofloxacin yields a well-defined and sensitive oxidation peak at the carbon paste electrode (CPE). Compared with that in the absence of SDBS, the oxidation peak current of ciprofloxacin remarkably increases in the presence of SDBS. The experimental parameters, such as supporting electrolyte, concentration of SDBS, and accumulation time, were optimized for ciprofloxacin determination. The oxidation peak current is proportional to the concentration of ciprofloxacin over the range from 8.0 × 10 −8 to 5.0 × 10 −6 mol L −1 . The detection limit is 2.0 × 10 −8 mol L −1 after 2 min of accumulation. This new voltammetric method was successfully used to detect ciprofloxacin in drugs.

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A liquid chromatographic method, with fluorometric detection, for the determination of enrofloxacin and ciprofloxacin in plasma and endometrial tissue of mares

Cited by 42 publications

References 25 publications

Determination of enrofloxacin in chicken plasma by high performance liquid chromatography for pharmacokinetic studies

Determination of enrofloxacin in chicken plasma by high performance liquid chromatography for pharmacokinetic studies

Simultaneous determination of malachite green, enrofloxacin and ciprofloxacin in fish farming water and fish feed by liquid chromatography with solid-phase extraction

Electrochemical Determination of Ciprofloxacin Based on the Enhancement Effect of Sodium Dodecyl Benzene Sulfonate

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