A liquid bead array for the identification and characterization of fljB -positive and fljB -negative monophasic variants of Salmonella Typhimurium

Boland, Cécile; Hessche, Mieke Van; Mahillon, Jacques; Wattiau, Pierre

doi:10.1016/j.fm.2017.04.006

Cited by 7 publications

(11 citation statements)

References 35 publications

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“…In these deletions, the region adjacent to the 3 -end of IS26 (deletion starting point) varied between strains, while the region adjacent to the 5 -end (deletion ending point) was conserved. The IS26 insertion was found in the same position (i.e., 334 nucleotides upstream from the iroB gene) in fljB-negative strains isolated in the USA, South Korea and some European countries [18,36]. It is noteworthy that three strains of our work were fljB-positives (i.e., ∆fljAB13), even though they had IS26 inserted.…”

Section: Discussionsupporting

confidence: 53%

“…Since this method is highly time-consuming and entails an unnecessary manipulation of the pathogen, multiplex PCR is routinely used. This PCR amplifies the fljB-fljA intergenic region of the flagellin gene cluster [5] but is unable to differentiate the monophasic fljB-positive variant from S. Typhimurium [36]. In this study, we found that WGS can prevent the amplification of the long-inserted fragments in the fljAB operon.…”

Section: Discussionmentioning

confidence: 77%

“…However, as we have verified through this work, NGS technologies allow a complete characterization of Salmonella strains within a few days. As an alternative to WGS, in 2018, a liquid bead array was proposed for the identification and characterization of S. 4, [5],12:i:variants to achieve results in a rapid and simple data analysis [36]. In this work, we demonstrate that WGS can also be a rapid method that enhances traditional profiling efforts for the characterization of the monophasic variant, including the prediction of clinically relevant phenotypic traits such as antibiotic resistance genes, plasmids or virulence genes.…”

Section: Discussionmentioning

confidence: 99%

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Genotyping Study of Salmonella 4,[5],12:i:- Monophasic Variant of Serovar Typhimurium and Characterization of the Second-Phase Flagellar Deletion by Whole Genome Sequencing

et al. 2020

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After Salmonella Enteritidis and S. Typhimurium, S. 4,[5],12:i:- is the most reported serovar in human clinical cases. During the past 20 years, many tools have been used for its typing and second-phase flagellar deletion characterization. Currently, whole genome sequencing (WGS) and different bioinformatic programs have shown the potential to be more accurate than earlier tools. To assess this potential, we analyzed by WGS and in silico typing a selection of 42 isolates of S. 4,[5],12:i:- and S. Typhimurium with different in vitro characteristics. Comparative analysis showed that SeqSero2 does not differentiate fljB-positive S. 4,[5],12:i:- strains from those of serovar Typhimurium. Our results proved that the strains selected for this work were non-clonal S. 4,[5],12:i:- strains circulating in Spain. Using WGS data, we identified 13 different deletion types of the second-phase flagellar genomic region. Most of the deletions were generated by IS26 insertions, showing orientation-dependent conserved deletion ends. In addition, we detected S. 4,[5],12:i:- strains of the American clonal line that would give rise to the Southern European clone in Spain. Our results suggest that new S. 4,[5],12:i:- strains are continuously emerging from different S. Typhimurium strains via different genetic events, at least in swine products.

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Section: Discussionsupporting

confidence: 53%

Section: Discussionmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 99%

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Genotyping Study of Salmonella 4,[5],12:i:- Monophasic Variant of Serovar Typhimurium and Characterization of the Second-Phase Flagellar Deletion by Whole Genome Sequencing

et al. 2020

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“…For serovar identification, amplification of Salmonella -specific invA , and for phase 1 and 2 antigens, fliC , fljA , fljB , fliB-fliA and hin genes have been proposed [ 18 , 19 ]. Although, recently STM 4492 coding for a putative cytoplasmic protein, and malic acid dehydrogenase ( mdh ) genes have been suggested as more suitable targets for Typhimurium serovar identification [ 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

Salmonella Typhimurium in Iran: Contribution of molecular and IS200 PCR methods in variants detection

Khaltabadi

Shahrokhi²,

Ebrahimi‐Rad

et al. 2019

PLoS ONE

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Salmonella Typhimurium, a zoonotic pathogen, is regarded as a major health and economic concern worldwide. Recently, monophasic variants of this serovar have been significantly associated with human gastroenteritis outbreaks globally, making its accurate identification essential for epidemiological and control purposes. We have identified and analyzed 150 S. Typhimurium from 884 Salmonella genus isolated from humans, domestic animals, poultry, food items and abattoirs origins. The Salmonella isolates were obtained from Iranian National Veterinary Reference Laboratories of 9 provinces during 2007–2016, and from five hospitals in Tehran in 2015. The isolates were evaluated biochemically, serologically, and by PCR amplification of invA, mdh, STM4492, fliC, fljA, fljB, hin genes, IS200 and DT104. invA and mdh genes were used to confirm the S. Typhimurium serotype, fliC and fljB genes for determination of monophasic variants and amplification of IS200 to discriminate the monophasic variants from the closely related serotypes. We identified 78.6% (118/150) as classical S. Typhimurium (fliC, fljB and IS200 positive), 12.6% (19/150) were IS200 negative from all isolates. DT104 is another marker for S.Typhimurium serovar typing. Contrary to EFSA guidelines 20.6% (19/29) of human isolates that lacked IS200 insertion sequence, were confirmed as S.Typhimurium. Compared to the North American/European isolates the low prevalence of fljB negative 6% (9/150) and the high abundance of fliC negative 23.3% (35/150) isolates also were indicative of a different regional atypical population. Studies have shown that the prevalence of monophasic (fljB-) S. Typhimurium worldwide is promoted by the Swine industry. Thus, one reason for this high number of different atypical strains could be inhibition of swine breeding system (house hold and industry) in Iran. These results demonstrate a need for a modified identifying protocol to overcome the regional differences.

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“…In most STMV strains isolated worldwide from humans, animals, foods and environmental sources an IS26 copy was detected next to iroB (Boland et al, 2018). This suggested a possible common ancestor for the globally observed STMV identified in recent years.…”

Section: Discussionmentioning

confidence: 86%

Antimicrobial resistance gene shuffling and a three-element mobilisation system in the monophasic Salmonella typhimurium strain ST1030

Oliva

Calia

Ferrara

et al. 2020

Plasmid

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A liquid bead array for the identification and characterization of fljB -positive and fljB -negative monophasic variants of Salmonella Typhimurium

Cited by 7 publications

References 35 publications

Genotyping Study of Salmonella 4,[5],12:i:- Monophasic Variant of Serovar Typhimurium and Characterization of the Second-Phase Flagellar Deletion by Whole Genome Sequencing

Genotyping Study of Salmonella 4,[5],12:i:- Monophasic Variant of Serovar Typhimurium and Characterization of the Second-Phase Flagellar Deletion by Whole Genome Sequencing

Salmonella Typhimurium in Iran: Contribution of molecular and IS200 PCR methods in variants detection

Antimicrobial resistance gene shuffling and a three-element mobilisation system in the monophasic Salmonella typhimurium strain ST1030

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