Abstract:Lipase is a type of enzyme that decomposes and synthesizes triglyceride on hydrophilic and lipophilic interface, which plays an important role in lipid processing. A novel heat-resisting lipase gene (lip4) in Thermomyces lanuginosus was cloned to the expression vector pPICZαA and then transported into Pichia pastoris X33 for high-efficiency expression. The structure of this lipase (Lip4) was analyzed by multiple bioinformatics software. Results showed that the recombinant yeast cell concentration reached the p… Show more
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