A linkage map of the porcine genome from a large‐scale White Duroc × Erhualian resource population and evaluation of factors affecting recombination rates

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Gestation length and maternal ability are important to improve the sow reproduction efficiency and their offspring survival. To map quantitative trait loci (QTL) for gestation length and maternal ability related traits including piglet survival rate and average body weight of piglets at weaning, more than 200 F 2 sows from a White Duroc 3 Erhualian resource population were phenotyped. A genome-wide scan was performed with 194 microsatellite markers covering the whole pig genome. QTL analysis was carried out using a composite regression interval mapping method via QTL express. The results showed that total number of born piglets was significantly correlated with gestation length (r 5 20.13, P , 0.05). Three QTL were detected on pig chromosome (SSC)2, 8 and 12 for gestation length. The QTL on SSC2 achieved the 5% genome-wide significant level and the QTL on SSC8 was consistent with previous reports. Four suggestive QTL were identified for maternal ability related traits including 1 QTL for survival rate of piglets at weaning on SSC8, 3 QTL for average body weight of piglet at weaning on SSC3, 11 and 13.

Section: Discussionmentioning

confidence: 99%

“…Primers were labeled with fluorescent dye (HEX, NED or FAM). All founder animals, F 1 individuals and 280 F 2 sows were genotyped for the 194 markers as described previously in Guo et al (2009).…”

Section: Methodsmentioning

confidence: 99%

A whole genome scan to detect quantitative trait loci for gestation length and sow maternal ability related traits in a White Duroc × Erhualian F2 resource population

Chen

Guo

Zhang

et al. 2010

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“…The F 2 resource population consist of 1912 F 2 animals that were derived from two White Duroc and 17 Erhualian as founders (Guo et al, 2009). The 15 outbred populations represent a broad genetic background of Chinese and Western pigs (Yan et al, 2011) (Table 1).…”

Section: Methodsmentioning

confidence: 99%

A genome-wide association analysis for susceptibility of pigs to enterotoxigenic Escherichia coli F41

Yang

Zhang

et al. 2016

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Enterotoxigenic Escherichia coli (ETEC) is a type of pathogenic bacteria that cause diarrhea in piglets through colonizing pig small intestine epithelial cells by their surface fimbriae. Different fimbriae type of ETEC including F4, F18, K99 and F41 have been isolated from diarrheal pigs. In this study, we performed a genome-wide association study to map the loci associated with the susceptibility of pigs to ETEC F41 using 39454 single nucleotide polymorphisms (SNPs) in 667 F 2 pigs from a White Duroc × Erhualian F 2 cross. The most significant SNP (ALGA0022658, P = 5.59 × 10 −13 ) located at 6.95 Mb on chromosome 4. ALGA0022658 was in high linkage disequilibrium ( r 2 > 0.5) with surrounding SNPs that span a 1.21 Mb interval. Within this 1.21 Mb region, we investigated ZFAT as a positional candidate gene. We re-sequenced cDNA of ZFAT in four pigs with different susceptibility phenotypes, and identified seven coding variants. We genotyped these seven variants in 287 unrelated pigs from 15 diverse breeds that were measured with ETEC F41 susceptibility phenotype. Five variants showed nominal significant association ( P < 0.05) with ETEC F41 susceptibility phenotype in International commercial pigs. This study provided refined region associated with susceptibility of pigs to ETEC F41 than that reported previously. Further works are needed to uncover the underlying causal mutation(s).

“…All animals in the White Duroc 3 Erhualian resource population were genotyped for a total of 183 informative microsatellite markers covering the whole pig genome. Amplification was performed for each locus as described in Guo et al (2009). Genotype data were recorded in an ABI PRISM R 3130XL Genetic Analyzer (Applied Biosystems, Foster City, CA, USA) with GeneMapper version 3.7, and visually inspected by two persons to assess the accuracy of genotype assignment.…”

Section: Animalsmentioning

confidence: 99%

“…The comprehensive linkage map was constructed with CRIMAP version 2.4 (Green et al, 1990). The number of markers on each chromosome varied from 5 on pig chromosome (SSC) 18, to 24 on SSC13 with a total length of 2344.7 cM and an average interval of 13.40 cM (Guo et al, 2009). …”

Section: Animalsmentioning

confidence: 99%

Detection of quantitative trait loci for porcine susceptibility to enterotoxigenic Escherichia coli F41 in a White Duroc × Chinese Erhualian resource population

Yang

Huang

Yan

et al. 2009

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Enterotoxigenic Escherichia coli (ETEC) is a major cause of diarrhoea in neonatal and postweaning pigs. F41 is one of ETEC fimbriae that adhere to the small intestinal epithelium and lead to development of diarrhoea. The genetic architecture of susceptibility to ETEC F41 remains elusive in pigs. In this study, we determined the in vitro adhesion phenotypes of ETEC F41 in a total of 835 F 2 animals from a White Duroc 3 Erhualian intercross, and performed a genome scan using both F 2 and half-sib analyses with 183 microsatellite markers to detect quantitative trait loci (QTL) for porcine susceptibility to ETEC F41. The two analyses consistently revealed a 1% genome-wide significant QTL on pig chromosome 4. Moreover, we determined F41 adhesion phenotypes in 14 purebred Erhualian and 14 White Duroc pigs. The results showed that both the founder breeds are segregating for the F41 adhesion phenotype, while less percentage of Erhualian pigs were adhesive to ETEC F41 compared to White Duroc pigs.