A Linkage Map of the Diploid Strawberry, Fragaria vesca

The History Of Genome Mapping In Fragaria Spp.

“…The broad range of distribution and cultivation of dessert strawberry is a consequence of its genotypic diversity and highly heterozygous nature, resulting in high adaptive capacity of this species (Kumar et al 2011). Although still unclear the interpretation of phylogenetic relations between F. × ananassa and diploid Fragaria (Potter et al 2000) allow to classify diploid F. vesca and F. innumae , as well as F. viridis, F. bucharica and F. pentaphylla among ancestors of cultivated strawberry (Davis & Yu 1997;. Complicated allopolyploid composition of strawberry genome makes it an interesting but very challenging object for genetic mapping.…”

“…In genetic mapping of diploid Fragaria plants, F2 population obtained from self-pollination or intercross of F1 has been used most frequently (Williamson et al 1995;Davis & Yu 1997;Deng & Davis 2001;Sargent et al 2004Sargent et al , 2006Sargent et al , 2007Sargent et al , 2008Sargent et al , 2011Ruiz-Rojas et al 2010;Illa et al 2011;Zorrilla-Fontanesi et al 2011b;Koskela et al 2012). However, some outline maps of F. vesca and F. viridis were elaborated on the basis of backcross population (BC), generated by crossing F1 individual(s) with inbred parent used in the initial cross (Nier et al 2006;Cekic et al 2001;Albani et al 2004).…”

“…The majority of segregating populations used for mapping genomes of Fragaria species contained 50 to 100 individuals Davis & Yu 1997;Haymes et al 1997;Sargent et al 2004Sargent et al , 2006Sargent et al , 2007Nier et al 2006;Rousseau-Gueutin et al 2008;Koskela et al 2012). Since more abundant progeny carries a greater number of meiotic recombination events, thus, it determines the resolution and accuracy of the linkage map more precisely (Paterson 1996), larger segregating populations of over 100 individuals were introduced in the study on F. vesca (Koskela et al 2012), F. virginiana and F. chiloensis (Spigler et al 2008Goldberg et al 2010) as well as F. × ananassa (Lerceteau-Köhler et al 2003, 2012Rousseau-Gueutin et al 2008Weebadde et al 2008;Sargent et al 2009Isobe et al 2012;Gaston et al 2013;van Dijk et al 2014).…”

“…Scaffolds of the maps were built based on RAPD (Random Amplified Polymorphic DNA) (Davis & Yu 1997;Sugimoto et al 2005), AFLP (Amplified Fragment Length Polymorphism) (Lerceteau-Köhler et al 2003Weebadde et al 2008;Sargent et al 2009), and ISSR (Inter-Simple Sequence Repeat) markers (Cekic et al 2001;Albani et al 2004). However, markers described above have many drawbacks, such as low reliability and insufficient level of polymorphism (RAPD), dominant inheritance (RAPD, AFLP) (Paran & Michelmore 1993), and labourintensive data scoring (ISSR) (Cekic et al 2001).…”

“…vesca 'Baron Solemacher' (a highly homozygous inbred line) × F. vesca ssp. americana 'WC6' population (Davis & Yu 1997 LGs with a total genetic distance of 448 cM. Subsequently, this reference map has been improved Illa et al 2011;Zorrilla-Fontanesi et al 2011b) and it latest version contains a total of 700 sequence tag sites derived from SSRs, genespecific markers, RFLPs, SNPs and ESTs, spanning along all seven linkage groups and covering a genetic distance of 442.8 cM .…”