A linear standard curve for the Folin Lowry determination of protein

Stauffer, Clyde E.

doi:10.1016/0003-2697(75)90172-4

Cited by 138 publications

(29 citation statements)

References 3 publications

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“…The mixture was centrifuged at 5000 rpm for 10 min and the acid soluble fraction obtained in the supernatant was subjected to protein determination by the Folin-Lowry method (Stauffer, 1975). Enzyme blanks were prepared by adding the TCA solution to the reaction mixture immediately after substrate addition.…”

Section: Antiproteolytic Assaymentioning

confidence: 99%

Evaluation of Crataeva nurvala extracts as antioxidant, antiproteolytic and cytotoxic against hepato-carcinoma and mouse melanoma cell lines

Hade¹,

Joshi²,

Pilley³

et al. 2016

J App Pharm Sci

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The crude extracts from stem bark of Crataeva nurvala Buch.-Ham containing naturally occurring antioxidants were screened for their most abundant phytosecondary metabolite, antiproteolytic and cytotoxic properties. The antioxidant activity of the extracts was determined by DPPH radical scavenging activity, Ferric reducing power and β Carotene linoleic acid assay. The antiproteolytic activity of crude extracts was determined by inhibition of trypsin-induced hydrolysis of BSA. The IC50 of cytotoxic effect on HepG2 and B16F0 cell line was investigated using MTT assay. Preliminary phytochemical analysis exhibited the presence of terpenoids as a major phytochemical reflecting higher antioxidant activity in a dose dependent manner. The PEECN extract showed good antiproteolytic activity with 26.04% inhibition on BSA. The IC50 values of PEECN against HepG2 and B16F0 cells using MTT assay were determined to be 34.67 ± 1.10 μg/ml and 49.43 ± 4.778 μg/ml after 48 h, respectively. These results demonstrate that the petroleum ether extract containing most terpenoid fraction exhibited potential antiproteolytic and cytotoxic activity against hepatocellular carcinoma and mouse melanoma in vitro through inhibition of proliferating cancerous cells.

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Section: Antiproteolytic Assaymentioning

confidence: 99%

Evaluation of Crataeva nurvala extracts as antioxidant, antiproteolytic and cytotoxic against hepato-carcinoma and mouse melanoma cell lines

Hade¹,

Joshi²,

Pilley³

et al. 2016

J App Pharm Sci

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“…1316 The BC3H-1 membranes, suspended in 50 mM Tris-HCl (pH 7.6) and 10 mM MgCl 2 , were incubated in a final volume of 0. 25 …”

Section: Radioligand Binding Assaysmentioning

confidence: 99%

“…25 Bovine serum albumin, containing sodium azide (1 mg/ml) as a noninterfering bactericide, was employed for standards.…”

Section: Protein Determinationmentioning

confidence: 99%

Certain beta-blockers can decrease beta-adrenergic receptor number: II. Down-regulation of receptor number by alprenolol and propranolol in cultured lymphoma and muscle cells.

Hughes

Mahan

Insel

1988

Circulation Research

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“…The cells were harvested, washed in 0.1 Mphosphate buffer pH 7.2, resuspended in the same buffer to an OD,,, of 1, and then ultrasonified with a Braunsonic sonifier. The cell extracts were centrifuged at 20000g for 20 min, and adjusted to a protein concentration of approximately 2 mg ml-l using the Lowry protein assay method (Stauffer, 1975). A 25 ml volume of this preparation was used directly as a reaction mixture to which 0.5 pmol substrate (2,3-OH-4-CB) was added to initiate the reaction.…”

Section: Methodsmentioning

confidence: 99%

Bioconversion of 2-hydroxy-6-oxo-6-(4'-chlorophenyl) hexa-2,4-dienoic acid, the meta-cleavage product of 4-chlorobiphenyl

Ahmad

Sylvestre

Sondossi

et al. 1991

Journal of General Microbiology

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Bacterial conversion of 4-chlorobiphenyl(4-CB) usually proceeds through a pathway involving an initial oxidation of the unsubstituted ring in the 2,3 position followed by a 1,2 meta-cleavage. The meta-cleavage product (MCP) is converted through a single hydrolysis step into chlorobenzoic acid. However, several other acidic metabolites that were not expected as part of this pathway have already been described. In this paper, we used strains of Pseudomonas putida carrying cloned genes from Pseudomonas testosteroni B-356 that are involved in polychlorinated biphenyl (PCB) degradation to demonstrate that several acidic metabolites found in the culture media of various bacteria grown in the presence of 4-CB result from alternative novel bioconversion pathways of MCP. The degradation products of MCP through these pathways were identified as analogues with saturated or shorter side chains or as 4-chlorophenyl-2-picolinic acid; pathways leading to their formation are proposed.

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A linear standard curve for the Folin Lowry determination of protein

Cited by 138 publications

References 3 publications

Evaluation of Crataeva nurvala extracts as antioxidant, antiproteolytic and cytotoxic against hepato-carcinoma and mouse melanoma cell lines

Evaluation of Crataeva nurvala extracts as antioxidant, antiproteolytic and cytotoxic against hepato-carcinoma and mouse melanoma cell lines

Certain beta-blockers can decrease beta-adrenergic receptor number: II. Down-regulation of receptor number by alprenolol and propranolol in cultured lymphoma and muscle cells.

Bioconversion of 2-hydroxy-6-oxo-6-(4'-chlorophenyl) hexa-2,4-dienoic acid, the meta-cleavage product of 4-chlorobiphenyl

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