2004
DOI: 10.1021/bi036271e
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A Linear Correlation between the Energetics of Allosteric Communication and Protein Flexibility in the Escherichia coli Cyclic AMP Receptor Protein Revealed by Mutation-Induced Changes in Compressibility and Amide Hydrogen−Deuterium Exchange

Abstract: Amino acid substitutions at distant sites in the Escherichia coli cyclic AMP receptor protein (CRP) have been shown to affect both the nature and magnitude of the energetics of cooperativity of cAMP binding, ranging from negative to positive. In addition, the binding to DNA is concomitantly affected. To correlate the effects of amino acid substitutions on the functional energetics and global structural properties in CRP, the partial specific volume (v(o)), the coefficient of adiabatic compressibility (beta(s)(… Show more

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Cited by 55 publications
(86 citation statements)
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“…Our findings reported here are a successful attempt of studying CBD-A of the R-subunit by multidimensional solution NMR methods. Furthermore, the proposed model defines a mechanism that is highly conserved and thus relevant for cAMP recognition in other homologous CBDs coupled to effector proteins with diverse functions, such as transcription factors (catabolite-activator protein) (20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31), guanine nucleotide exchange factors (11), and ion channel proteins (both hyperpolarization-activated cyclic nucleotide-dependent channels and cyclic nucleotide-gated channels) (32,33). This model also serves as a general paradigm for how small molecules such as cAMP allosterically control large proteinprotein interfaces.…”
mentioning
confidence: 99%
“…Our findings reported here are a successful attempt of studying CBD-A of the R-subunit by multidimensional solution NMR methods. Furthermore, the proposed model defines a mechanism that is highly conserved and thus relevant for cAMP recognition in other homologous CBDs coupled to effector proteins with diverse functions, such as transcription factors (catabolite-activator protein) (20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31), guanine nucleotide exchange factors (11), and ion channel proteins (both hyperpolarization-activated cyclic nucleotide-dependent channels and cyclic nucleotide-gated channels) (32,33). This model also serves as a general paradigm for how small molecules such as cAMP allosterically control large proteinprotein interfaces.…”
mentioning
confidence: 99%
“…To approximate the properties of the ideal isolated protein molecules, which have no intermolecular interactions, the apparent specific volume is extrapolated to the limit of zero protein concentration and the partial specific volume of the protein molecule in the solution, o v , is obtained as equation 3 (Gekko and Noguchi, 1974;Millero et al, 1976;Gekko and Noguchi, 1979;Gekko and Hasegawa, 1986;Gekko and Hasegawa, 1989;Gekko and Yamagami, 1991;Kamiyama and Gekko, 2000;Gekko et al, 2003;Gekko et al, 2004;Chalikian et al, 1993;Blandamer et al, 2001;Taulier et al, 2005).…”
Section: Literature Reviewmentioning
confidence: 99%
“…For macromolecules such as protein molecules To RT M β is small and can be neglected (Stillinger, 1973;Kharakoz, 1992;Chalikian, 2003). (Gekko and Noguchi, 1979;Gekko and Hasegawa, 1986;Gekko and Hasegawa, 1989;Gekko and Yamagami, 1991;Kharakoz, 1992;Chalikian, 1998;Chalikian and Breslauer, 1998;Murphy et al, 1998;Chalikian, 2001;Gekko, 2002;Valdez et al, 2001;Chalikian, 2003;Gekko et al, 2003;Gekko et al, 2004;Bano and Marek, 2006 Taulier and Chalikian, 2002;Mori et al, 2006). …”
Section: Interpretation Of Protein Partial Specific Volumementioning
confidence: 99%
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