A Lineage of Begomoviruses Encode Rep and AC4 Proteins of Enigmatic Ancestry: Hints on the Evolution of Geminiviruses in the New World

Torres-Herrera, Sandra Iliana; Romero-Osorio, Angélica; Moreno‐Valenzuela, Oscar A.; Pastor-Palacios, Guillermo; Cárdenas-Conejo, Yair; Ramírez-Prado, Jorge Humberto; Riego-Ruíz, Lina; Minero-García, Yereni; Ambríz-Granados, Salvador; Argüello‐Astorga, Gerardo R.

doi:10.3390/v11070644

Cited by 17 publications

(11 citation statements)

References 60 publications

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“…All the proteins have the average size of BGVs proteins, with the exception of AC4. The AC4 protein is 130 aa in length, which is unusually long since the average size of AC4 protein is~85 aa for typical BGVs and 121 aa in length for BGVs belonging to SLCuV clade (Torres-Herrera et al, 2019). A BLASTP search revealed that only CuChLV and another three BGVs of the SLCuV clade have AC4 proteins with 130 aa (BLCrV: YP_009666510.1; Wissadula yellow mosaic virus: AOT83446.1; Abutilon golden mosaic Yucatan virus: YP_009506373.1).…”

Section: Genomic Characterization Of Cucumber Leaf Curl Virusmentioning

confidence: 99%

“…New World BGVs are grouped in several secondary linages or "clades," such as the Squash leaf curl virus (SLCuV) clade, the Abutilon mosaic virus (AbMV) clade and the Brazil clade (Argüello-Astorga et al, 1994;Rojas et al, 2005). Members of the SLCuV clade display distinctive features in their replication origin, AC4 protein and N-terminal domain of their Rep protein (Argüello-Astorga et al, 1994;Gregorio-Jorge et al, 2010;Torres-Herrera et al, 2019). Legumoviruses and sweepoviruses are grouped by the host that they specifically infect.…”

Section: Introductionmentioning

confidence: 99%

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First report of begomoviruses infectingCucumis sativusL. in North America and identification of a proposed new begomovirus species

Sanchez-Chavez

Regla-Márquez

Cardenas-Conejo

et al. 2020

PeerJ

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Background Members of the Begomovirus genus are phytopathogens that infect dicotyledonous plants, producing economic losses in tropical and subtropical regions. To date, only seven species of begomoviruses (BGVs) infecting cucumber have been described. Most cucumber infections were reported in South Asia. In the Americas, begomoviral infections affecting cucumber are scarce; just one report of begomovirus has been described in South America. The presence of whitefly and typical symptoms of viral infections observed in a cucumber field in Colima, Mexico, suggested that plants in this field were affected by BGVs. Methods To identify the BGVs infecting cucumber, we performed a high-throughput sequencing and compared the assembled contigs against the GenBank nucleic acid sequence database. To confirm the presence of viruses in cucumber samples, we performed a PCR detection using specific oligonucleotides. We cloned and sequenced by Sanger method the complete genome of a potential new begomovirus. Begomovirus species demarcation was performed according to the International Committee on Taxonomy of Viruses. The evolutionary relationship of the new virus was inferred using phylogenetic and recombination analyses. Results We identified five species of begomovirus infecting plants in a field. None of these have been previously reported infecting cucumber. One of the five species of viruses here reported is a new begomovirus species. Cucumber chlorotic leaf virus, the new species, is a bipartite begomovirus that has distinctive features of viruses belonging to the squash leaf curl virus clade. Conclusions The findings here described represent the first report of begomoviral infection affecting cucumber plants in North America. Previous to this report, only seven begomovirus species have been reported in the world, here we found five species infecting cucumber plants in a small sample suggesting that cucumber is vulnerable to BGVs. One of these viruses is a new species of begomovirus which is the first begomovirus originally isolated from the cucumber. The findings of this report could help to develop strategies to fight the begomoviral infections that affect cucumber crops.

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Section: Genomic Characterization Of Cucumber Leaf Curl Virusmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

First report of begomoviruses infectingCucumis sativusL. in North America and identification of a proposed new begomovirus species

Sanchez-Chavez

Regla-Márquez

Cardenas-Conejo

et al. 2020

PeerJ

Self Cite

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“…Monopartite BGVs encode six proteins, viz. C1/Rep, C2/TrAP, C3/REn, C4, V2, and V1/CP [ 9 ]. Homologs are encoded in one of the genomic components of bipartite BGVs, firstly DNA A (in this case, termed AC1/Rep, AC2/TrAP, AC3/REn, AC4, AV2, and AV1/CP); secondly, DNA B (the additional component in bipartite species) encodes two additional proteins: the nuclear shuttle protein (NSP) and the movement protein (MP).…”

Section: Introductionmentioning

confidence: 99%

“…The NW BGVs (~140 species) have a bipartite genome (DNA-A and DNA-B) with a few reported exceptions, while the OW BGVs include both bipartite and monopartite species, with a predominance of the latter (ca. 85%) [ 9 ]. A key difference between the New World (NW) and Old World (OW) BGVs is that the latter has an extra small open reading frame (ORF) that leads and moderately overlaps the CP gene, termed AV2/V2 or “precoat” gene [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Genomic Variation and Diversification in Begomovirus Genome in Implication to Host and Vector Adaptation

Nigam

2021

Plants

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Begomoviruses (family Geminiviridae, genus Begomovirus) are DNA viruses transmitted in a circulative, persistent manner by the whitefly Bemisia tabaci (Gennadius). As revealed by their wide host range (more than 420 plant species), worldwide distribution, and effective vector transmission, begomoviruses are highly adaptive. Still, the genetic factors that facilitate their adaptation to a diverse array of hosts and vectors remain poorly understood. Mutations in the virus genome may confer a selective advantage for essential functions, such as transmission, replication, evading host responses, and movement within the host. Therefore, genetic variation is vital to virus evolution and, in response to selection pressure, is demonstrated as the emergence of new strains and species adapted to diverse hosts or with unique pathogenicity. The combination of variation and selection forms a genetic imprint on the genome. This review focuses on factors that contribute to the evolution of Begomovirus and their global spread, for which an unforeseen diversity and dispersal has been recognized and continues to expand.

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“…[41]. Moreover, these malvaceous weeds are commonly infected by begomoviruses and develop striking golden/yellow mosaic symptoms [25,33,34,36,[42][43][44][45][46][47].…”

Section: Introductionmentioning

confidence: 99%

Weed-infecting viruses in a tropical agroecosystem present different threats to crops and evolutionary histories

et al. 2021

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In the Caribbean Basin, malvaceous weeds commonly show striking golden/yellow mosaic symptoms. Leaf samples from Malachra sp. and Abutilon sp. plants with these symptoms were collected in Hispaniola from 2014 to 2020. PCR tests with degenerate primers revealed that all samples were infected with a bipartite begomovirus, and sequence analyses showed that Malachra sp. plants were infected with tobacco leaf curl Cuba virus (TbLCuCV), whereas the Abutilon sp. plants were infected with a new bipartite begomovirus, tentatively named Abutilon golden yellow mosaic virus (AbGYMV). Phylogenetic analyses showed that TbLCuCV and AbGYMV are distinct but closely related species, which are most closely related to bipartite begomoviruses infecting weeds in the Caribbean Basin. Infectious cloned DNA-A and DNA-B components were used to fulfilled Koch’s postulates for these diseases of Malachra sp. and Abutilon sp. In host range studies, TbLCuCV also induced severe symptoms in Nicotiana benthamiana, tobacco and common bean plants; whereas AbGYMV induced few or no symptoms in plants of these species. Pseudorecombinants generated with the infectious clones of these viruses were highly infectious and induced severe symptoms in N. benthamiana and Malachra sp., and both viruses coinfected Malachra sp., and possibly facilitating virus evolution via recombination and pseudorecombination. Together, our results suggest that TbLCuCV primarily infects Malachra sp. in the Caribbean Basin, and occasionally spills over to infect and cause disease in crops; whereas AbGYMV is well-adapted to an Abutilon sp. in the Dominican Republic and has not been reported infecting crops.

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A Lineage of Begomoviruses Encode Rep and AC4 Proteins of Enigmatic Ancestry: Hints on the Evolution of Geminiviruses in the New World

Cited by 17 publications

References 60 publications

First report of begomoviruses infectingCucumis sativusL. in North America and identification of a proposed new begomovirus species

First report of begomoviruses infectingCucumis sativusL. in North America and identification of a proposed new begomovirus species

Genomic Variation and Diversification in Begomovirus Genome in Implication to Host and Vector Adaptation

Weed-infecting viruses in a tropical agroecosystem present different threats to crops and evolutionary histories

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