A LIM motif is present in a pollen-specific protein.

Baltz, Rachel; Evrard, Jean-Luc; Domon, Claire; Steinmetz, André

doi:10.1105/tpc.4.12.1465

Cited by 45 publications

(15 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Later in development, DE genes and GO terms have been demonstrated to be involved in pollen-specific responses, reproduction, and cell signaling, all of which may play a role in SI. During this stage of development, DE genes include pollen allergen ole e 6-like protein, ole e 1-like protein, pollen-specific protein sf3-like, and protein-tyrosine kinase 2-beta-like (Table 3), while overrepresented CC GO terms comprise extracellular region, cell wall, plasma membrane (Table 6) (Baltz et al, 1992a,b; Hubbard and Till, 2000; Staiger and Franklin-Tong, 2003; Jiménez-López et al, 2011; Lopez-Casado et al, 2012). This pattern of expression demonstrates a shift in the function of DE genes between early and late development.…”

Section: Discussionmentioning

confidence: 99%

De novo Sequencing and Comparative Transcriptomics of Floral Development of the Distylous Species Lithospermum multiflorum

Cohen

2016

Front. Plant Sci.

View full text Add to dashboard Cite

Genes controlling the morphological, micromorphological, and physiological components of the breeding system distyly have been hypothesized, but many of the genes have not been investigated throughout development of the two floral morphs. To this end, the present study is an examination of comparative transcriptomes from three stages of development for the floral organs of the morphs of Lithospermum multiflorum. Transcriptomes of flowers of the two morphs, from various stages of development, were sequenced using an Illumina HiSeq 2000. The floral transcriptome of L. multiflorum was assembled, and differential gene expression (DE) was identified between morphs, throughout development. Additionally, Gene Ontology (GO) terms for DE genes were determined. Fewer genes were DE early in development compared to later in development, with more genes highly expressed in the gynoecium of the SS morph and the corolla and androecium of the LS morph. A reciprocal pattern was observed later in development, and many more genes were DE during this latter stage. During early development, DE genes appear to be involved in growth and floral development, and during later development, DE genes seem to affect physiological functions. Interestingly, many genes involved in response to stress were identified as DE between morphs.

show abstract

Section: Discussionmentioning

confidence: 99%

De novo Sequencing and Comparative Transcriptomics of Floral Development of the Distylous Species Lithospermum multiflorum

Cohen

2016

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…They were originally described in animals (Way and Chal®e 1988;Freydt et al 1990; Karlsson et al 1990), but have also been detected in yeast (MuÈ ller et al 1994) and in plants (Baltz et al 1992a(Baltz et al , 1992bMundel et al 2000). LIM proteins contain one or several LIM domains ± double zinc-®nger motifs with the sequence C-X2-C-X17±19-H-X2-C-X2-C-X2-C-X16±24-C-X2-(C,H,D) ± which are often found in the same protein together with another functional domain, such as a homeodomain or a kinase domain .…”

Section: Introductionmentioning

confidence: 94%

Molecular and expression analysis of a LIM protein gene family from flowering plants

et al. 2000

View full text Add to dashboard Cite

LIM-domain proteins participate in important cellular processes in eukaryotes, including gene transcription and actin cytoskeleton organization. They are predominantly found in animals, but have also been identified in yeast and plants. Following the characterization ofa LIM-domain protein in sunflower pollen, we carried out an extensive search for these proteins in flowering plants. We have isolated and studied cDNAs and/or genomic sequences for two novel LIM-domain proteins from sunflower, three from tobacco, and one from Arabidopsis. The plant proteins are structurally related to the cytoskeleton-associated CRP class of LIM proteins in animals, but show several distinctive features, including a second, atypical, LIM domain. We have performed comparative expression studies of these genes, as well as of one other gene from tobacco and two additional Arabidopsis genes whose sequences are available from databases. These studies, carried out by RT-PCR in the presence of gene-specific primers, showed that, in sunflower and tobacco, pollen grains and sporophytic tissues express different sets of LIM proteins. With the exception of one Arabidopsis gene--which has two introns--all the genes analyzed contain four introns at conserved positions, indicating that the ancestral gene from which the various copies evolved in higher plants allready had this split structure.

show abstract

“…The two LIM domain-containing proteins from plants (plant LIMs) are short (~200-aa) proteins, which are structurally related to the vertebrate cysteine-rich proteins (CRPs) and characterized by two tandemly arranged LIM domains (Baltz et al, 1992; Weiskirchen and Gunther, 2003; Kadrmas and Beckerle, 2004; Arnaud et al, 2007). The term ‘LIM’ has derived from the first letter of the proteins LIN-11 (Freyd et al, 1990), Isl1 (Karlsson et al, 1990), and MEC-3 (Way and Chalfie, 1989) from which the LIM domain was historically identified.…”

Section: Introductionmentioning

confidence: 99%

A LIM Domain Protein from Tobacco Involved in Actin-Bundling and Histone Gene Transcription

et al. 2013

View full text Add to dashboard Cite

The two LIM domain-containing proteins from plants (LIMs) typically exhibit a dual cytoplasmic–nuclear distribution, suggesting that, in addition to their previously described roles in actin cytoskeleton organization, they participate in nuclear processes. Using a south-western blot-based screen aimed at identifying factors that bind to plant histone gene promoters, we isolated a positive clone containing the tobacco LIM protein WLIM2 (NtWLIM2) cDNA. Using both green fluorescent protein (GFP) fusion- and immunology-based strategies, we provide clear evidence that NtWLIM2 localizes to the actin cytoskeleton, the nucleus, and the nucleolus. Interestingly, the disruption of the actin cytoskeleton by latrunculin B significantly increases NtWLIM2 nuclear fraction, pinpointing a possible novel cytoskeletal–nuclear crosstalk. Biochemical and electron microscopy experiments reveal the ability of NtWLIM2 to directly bind to actin filaments and to crosslink the latter into thick actin bundles. Electrophoretic mobility shift assays show that NtWLIM2 specifically binds to the conserved octameric cis-elements (Oct) of the Arabidopsis histone H4A748 gene promoter and that this binding largely relies on both LIM domains. Importantly, reporter-based experiments conducted in Arabidopsis and tobacco protoplasts confirm the ability of NtWLIM2 to bind to and activate the H4A748 gene promoter in live cells. Expression studies indicate the constitutive presence of NtWLIM2 mRNA and NtWLIM2 protein during tobacco BY-2 cell proliferation and cell cycle progression, suggesting a role of NtWLIM2 in the activation of basal histone gene expression. Interestingly, both live cell and in vitro data support NtWLIM2 di/oligomerization. We propose that NtWLIM2 functions as an actin-stabilizing protein, which, upon cytoskeleton remodeling, shuttles to the nucleus in order to modify gene expression.

show abstract

A LIM motif is present in a pollen-specific protein.

Cited by 45 publications

References 11 publications

De novo Sequencing and Comparative Transcriptomics of Floral Development of the Distylous Species Lithospermum multiflorum

De novo Sequencing and Comparative Transcriptomics of Floral Development of the Distylous Species Lithospermum multiflorum

Molecular and expression analysis of a LIM protein gene family from flowering plants

A LIM Domain Protein from Tobacco Involved in Actin-Bundling and Histone Gene Transcription

Contact Info

Product

Resources

About