A light-sheet microscope compatible with mobile devices for label-free intracellular imaging and biosensing

Wu, Tsung-Feng; Yen, Tony; Han, Yuanyuan; Chiu, Yu-Jui; Lin, Eason Y.-S.; Lo, Yu‐Hwa

doi:10.1039/c4lc00257a

Cited by 23 publications

(19 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To observe cells in different stages, A549 cells are cultured with inhibitors to stop their growth at different development stages 29 . Mitomycin is used to stop the cell growth at G1 phase where the biosynthetic activities of cells are activated to form necessary proteins for the next phase (S phase).…”

Section: Resultsmentioning

confidence: 99%

Imaging Cells in Flow Cytometer Using Spatial-Temporal Transformation

Han

2015

Sci Rep

Self Cite

View full text Add to dashboard Cite

Flow cytometers measure fluorescence and light scattering and analyze multiple physical characteristics of a large population of single cells as cells flow in a fluid stream through an excitation light beam. Although flow cytometers have massive statistical power due to their single cell resolution and high throughput, they produce no information about cell morphology or spatial resolution offered by microscopy, which is a much wanted feature missing in almost all flow cytometers. In this paper, we invent a method of spatial-temporal transformation to provide flow cytometers with cell imaging capabilities. The method uses mathematical algorithms and a spatial filter as the only hardware needed to give flow cytometers imaging capabilities. Instead of CCDs or any megapixel cameras found in any imaging systems, we obtain high quality image of fast moving cells in a flow cytometer using PMT detectors, thus obtaining high throughput in manners fully compatible with existing cytometers. To prove the concept, we demonstrate cell imaging for cells travelling at a velocity of 0.2 m/s in a microfluidic channel, corresponding to a throughput of approximately 1,000 cells per second.

show abstract

Section: Resultsmentioning

confidence: 99%

Imaging Cells in Flow Cytometer Using Spatial-Temporal Transformation

Han

2015

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…The tilted narrow beam was used to produce scattered light from the agglutination. The detail of fabrication can be found in our previous study [28]. The design of microfluidic device is illustrated in Figure 1h, which includes an inlet, a curving microchannel, a viewing window as the sensing area, a waste collection reservoir and an outlet.…”

Section: Methodsmentioning

confidence: 99%

“…However, some protocols require fluorescent labeling, which complicates sample preparation and discourage mobile platforms from the field testing of bacterial detection. To satisfy such challenges without significantly compromising sensitivity and specificity of detection, we propose a facile protocol including the following key features: (1) pre-enrichment of bacteria based on centrifugal membrane filtration; (2) quantitative detection of specific pathogen utilizing immunoagglutination reaction which is a one-step reaction and capable of achieving high selectivity and specificity without multiple washing steps [26,27]; and (3) a capillary driven microfluidic device, which transports introduced fluid sample without using external pump and also utilizes the narrow beam scanning technique compatible with the ubiquitous mobile electronics ideal for the purpose of waterborne field testing [28]. Due to the fact that E. coli O157:H7 can lead to serious human health issues such as bloody diarrhea, kidney failure, and even death, we have chosen this pathogen as the testing target and demonstrated the sensitivity and portability of our platform in this study [29,30,31].…”

Section: Introductionmentioning

confidence: 99%

Rapid Waterborne Pathogen Detection with Mobile Electronics

Wu¹,

Chen²,

Wang³

et al. 2017

Sensors

Self Cite

View full text Add to dashboard Cite

Pathogen detection in water samples, without complex and time consuming procedures such as fluorescent-labeling or culture-based incubation, is essential to public safety. We propose an immunoagglutination-based protocol together with the microfluidic device to quantify pathogen levels directly from water samples. Utilizing ubiquitous complementary metal–oxide–semiconductor (CMOS) imagers from mobile electronics, a low-cost and one-step reaction detection protocol is developed to enable field detection for waterborne pathogens. 10 mL of pathogen-containing water samples was processed using the developed protocol including filtration enrichment, immune-reaction detection and imaging processing. The limit of detection of 10 E. coli O157:H7 cells/10 mL has been demonstrated within 10 min of turnaround time. The protocol can readily be integrated into a mobile electronics such as smartphones for rapid and reproducible field detection of waterborne pathogens.

show abstract

“…The on-chip flow cytometry of leukocyte cells was demonstrated with the design of a compact in-line DH microscope using a pulsed, fiber-coupled laser source 22 . The light sheet microscope is a good example of exploiting the microfluidic environment for cytometry and imaging purposes 23 . In Refs.…”

Section: Introductionmentioning

confidence: 99%

Endowing a plain fluidic chip with micro-optics: a holographic microscope slide

et al. 2017

View full text Add to dashboard Cite

Lab-on-a-Chip (LoC) devices are extremely promising in that they enable diagnostic functions at the point-of-care. Within this scope, an important goal is to design imaging schemes that can be used out of the laboratory. In this paper, we introduce and test a pocket holographic slide that allows digital holography microscopy to be performed without an interferometer setup. Instead, a commercial off-the-shelf plastic chip is engineered and functionalized with this aim. The microfluidic chip is endowed with micro-optics, that is, a diffraction grating and polymeric lenses, to build an interferometer directly on the chip, avoiding the need for a reference arm and external bulky optical components. Thanks to the single-beam scheme, the system is completely integrated and robust against vibrations, sharing the useful features of any common path interferometer. Hence, it becomes possible to bring holographic functionalities out of the lab, moving complexity from the external optical apparatus to the chip itself. Label-free imaging and quantitative phase contrast mapping of live samples are demonstrated, along with flexible refocusing capabilities. Thus, a liquid volume can be analyzed in one single shot with no need for mechanical scanning systems.

show abstract

A light-sheet microscope compatible with mobile devices for label-free intracellular imaging and biosensing

Cited by 23 publications

References 42 publications

Imaging Cells in Flow Cytometer Using Spatial-Temporal Transformation

Imaging Cells in Flow Cytometer Using Spatial-Temporal Transformation

Rapid Waterborne Pathogen Detection with Mobile Electronics

Endowing a plain fluidic chip with micro-optics: a holographic microscope slide

Contact Info

Product

Resources

About