A light sheet fluorescence microscopy protocol for <i>Caenorhabditis elegans</i> larvae and adults

Smith, Jayson J.; Kenny, Isabel W.; Wolff, Carsten; Cray, Rachel; Kumar, Abhishek; Sherwood, David R.; Matus, David Q.

doi:10.1101/2022.08.05.503008

Cited by 1 publication

(1 citation statement)

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“…A low-cost option is similarly available by substituting a DC stepper motor and a driver with micro-stepping capability to permit smooth acceleration. Both options would also permit compatibility with current platforms for in vivo imaging in Drosophila [50] and Caenorhabditis [51]. Naturally, our approach is compatible with head-mounted microscopes [52,53] and stably-mounted high-density probes of electrical activity [54] in rodents.…”

Section: Ways To Extend Tipmmentioning

confidence: 99%

Tilt In Place Microscopy (TIPM): a simple, low-cost solution to image neural responses to body rotations

Hamling

Zhu

Auer

et al. 2022

Preprint

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Animals use information about gravity and other destabilizing forces to balance and navigate through their environment. Measuring how brains respond to these forces requires considerable technical knowledge and/or financial resources. We present a simple alternative: Tilt In Place Microscopy (TIPM). TIPM is a low-cost and non-invasive way to measure neural activity following rapid changes in body orientation. Here we used TIPM to study vestibulospinal neurons in larval zebrafish during and immediately after roll tilts. Vestibulospinal neurons responded with reliable increases in activity that varied as a function of ipsilateral tilt amplitude. TIPM differentiated tonic (i.e. sustained tilt) from phasic responses, revealing coarse topography of stimulus sensitivity in the lateral vestibular nucleus. Neuronal variability across repeated sessions was minor relative to trial-to-trial variability, allowing us to use TIPM for longitudinal studies of the same neurons across two developmental timepoints. There, we observed global increases in response strength, and systematic changes in the neural representation of stimulus direction. Our data extend classical characterization of the body tilt representation by vestibulospinal neurons and establish TIPM's utility to study the neural basis of balance, especially in developing animals.

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