A light-sensitive mutation in Arabidopsis LEW3 reveals the important role of N-glycosylation in root growth and development

Manzano, Concepción; Pallero-Baena, Mercedes; Silva‐Navas, Javier; Neila, Sara Navarro; Casimiro, Ilda; Casero, P. J.; García-Mina, José M.; Baigorri, Roberto; Rubio, Lourdes; Fernández, José Antonio; Norris, Matthew; Ding, Yiliang; Moreno-Risueño, Miguel A.; Pozo, Juan C. del

doi:10.1093/jxb/erx324

Cited by 3 publications

(3 citation statements)

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“…However, the majority of these analyses were done with the root system growing in the presence of light. We previously showed that root illumination generates a stress (Silva‐Navas et al ., , ; Manzano et al ., ). In this work, we demonstrate that illumination is an additive stress to Pi starvation.…”

Section: Discussionmentioning

confidence: 97%

Role of cis‐zeatin in root responses to phosphate starvation

Silva‐Navas

Conesa

Saéz³

et al. 2019

New Phytologist

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Summary Phosphate (Pi) is an essential nutrient for all organisms. Roots are underground organs, but the majority of the root biology studies have been done on root systems growing in the presence of light. Root illumination alters the Pi starvation response (PSR) at different intensities. Thus, we have analyzed morphological, transcriptional and physiological responses to Pi starvation in dark‐grown roots. We have identified new genes and pathways regulated by Pi starvation that were not described previously. We also show that Pi‐starved plants increase the cis‐zeatin (cZ) : trans‐zeatin (tZ) ratio. Transcriptomic analyses show that tZ preferentially represses cell cycle and PSR genes, whereas cZ induces genes involved in cell and root hair elongation and differentiation. In fact, cZ‐treated seedlings show longer root system as well as longer root hairs compared with tZ‐treated seedlings, increasing the total absorbing surface. Mutants with low cZ concentrations do not allocate free Pi in roots during Pi starvation. We propose that Pi‐starved plants increase the cZ : tZ ratio to maintain basal cytokinin responses and allocate Pi in the root system to sustain its growth. Therefore, cZ acts as a PSR hormone that stimulates root and root hair elongation to enlarge the root absorbing surface and to increase Pi concentrations in roots.

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Section: Discussionmentioning

confidence: 97%

Role of cis‐zeatin in root responses to phosphate starvation

Silva‐Navas

Conesa

Saéz³

et al. 2019

New Phytologist

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“…A genetic screen to identify plants with an altered root system was carried out using an EMS‐mutagenized SKP2Bp::GUS marker population (Manzano et al ., , ). Mutants displaying root development defects or altered expression of SKP2B (S‐phase kinase‐associated protein 2) were identified.…”

Section: Resultsmentioning

confidence: 99%

The polyadenylation factor FIP1 is important for plant development and root responses to abiotic stresses

et al. 2019

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Root development and its response to environmental changes is crucial for whole plant adaptation. These responses include changes in transcript levels. Here, we show that the alternative polyadenylation (APA) of mRNA is important for root development and responses. Mutations in FIP1, a component of polyadenylation machinery, affects plant development, cell division and elongation, and response to different abiotic stresses. Salt treatment increases the amount of poly(A) site usage within the coding region and 5 0 untranslated regions (5 0 -UTRs), and the lack of FIP1 activity reduces the poly(A) site usage within these non-canonical sites. Gene ontology analyses of transcripts displaying APA in response to salt show an enrichment in ABA signaling, and in the response to stresses such as salt or cadmium (Cd), among others. Root growth assays show that fip1-2 is more tolerant to salt but is hypersensitive to ABA or Cd. Our data indicate that FIP1-mediated alternative polyadenylation is important for plant development and stress responses.

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“…Glycosyltransferases perform N-glycosylation, a post-translational protein modification involved in various biological processes [ 24 – 27 ]. N-glycosylation plays a positive role in the development of the cell wall [ 24 ], leaves [ 25 ], and roots [ 26 ]. α - 1,2-Glucosyltransferase (ALG10) is a key enzyme that participates in the final step of liposaccharide formation, catalyzing the transfer of the last glucosyl molecule to a liposaccharide precursor to generate oligosaccharide chains in the N-glycan biosynthesis pathway [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

BoALG10, an α-1,2 glycosyltransferase, plays an essential role in maintaining leaf margin shape in ornamental kale

Feng

Yang

Zhong

et al. 2022

Horticulture Research

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The morphological diversity of leaf margin shapes is an identifying characteristic of many plant species. In our previous work, BoALG10 (α-1,2 glycosyltransferase) was predicted to be a key regulator of leaf margin shape in ornamental kale (Brassica oleracea var. acephala). An alanine and a leucine residues in the conserved domain of the smooth-margined S0835 were substituted by an aspartate and a phenylalanine, respectively, in the corresponding positions of the feathered-margined F0819. However, the expression pattern and function of this gene remain unclear. Here, we examined the expression patterns of BoALG10 using quantitative real-time PCR, and statistically significant differences of expression existed in nine developmental stages between F0819 and S0835. The BoALG10 protein localized to endoplasmic reticulum. The function of BoALG10 was then examined using complementary mutant assays. The overexpression strains phenocopied the smooth leaf margin after introduced the BoALG10S0835 into the feathered-margined inbred line F0819. Simultaneously, irregular dissections appeared in the leaf margins of knockout mutants KO-1 and KO-2 which generated by CRISPR/Cas9 technology from the smooth-margined inbred line S0835. A microscopic observation showed that the leaf margin cells of the smooth-margined plants S0835 and OE-3 were arranged regularly, while the cells of the feathered-margined plants F0819 and KO-1 were of inconsistent size and distributed in an irregular manner, particularly around the indentations of the leaf. This elucidation of BoALG10 function provides a novel insight into the morphological regulation of leaf margin shape.

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A light-sensitive mutation in Arabidopsis LEW3 reveals the important role of N-glycosylation in root growth and development

Cited by 3 publications

References 61 publications

Role of cis‐zeatin in root responses to phosphate starvation

Role of cis‐zeatin in root responses to phosphate starvation

The polyadenylation factor FIP1 is important for plant development and root responses to abiotic stresses

BoALG10, an α-1,2 glycosyltransferase, plays an essential role in maintaining leaf margin shape in ornamental kale

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