A light-regulated bio-micro-actuator powered by transgenic Drosophila melanogaster muscle tissue

Suzumura, Kiyofumi; Funakoshi, Kei; Hoshino, Takayuki; Tsujimura, Hidenobu; Iwabuchi, Kikuo; Akiyama, Yoshitake; Morishima, Keisuke

doi:10.1109/memsys.2011.5734383

Cited by 14 publications

(8 citation statements)

References 10 publications

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“…This maintenance is a major drawback to using mammalian muscle cells in bio‐hybrid applications. As a viable alternative, insect muscle cells have recently been proposed for use in a bio‐hybrid actuator . Insect muscle cells are more environmentally robust.…”

Section: Bio‐hybrid Methodsmentioning

confidence: 99%

“…Recently, optical control methods have also been applied to muscle cells using a genetic modification technique termed optogenetics . In this method, muscle cells are genetically modified to express a light‐activated cation channel that when evoked with the proper wavelength of light can initiate the contraction of the cells .…”

Section: Bio‐hybrid Methodsmentioning

confidence: 99%

“…In this method, muscle cells are genetically modified to express a light‐activated cation channel that when evoked with the proper wavelength of light can initiate the contraction of the cells . This technique has been successfully applied to cardiomyocytes, skeletal muscle cells, and insect muscle cells . Bruegmann et al showed that cardiomyocytes could be stimulated with light pulses as short as 1 ms .…”

Section: Bio‐hybrid Methodsmentioning

confidence: 99%

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Bio‐Hybrid Cell‐Based Actuators for Microsystems

Carlsen

Sitti

2014

Small

198

199

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As we move towards the miniaturization of devices to perform tasks at the nano and microscale, it has become increasingly important to develop new methods for actuation, sensing, and control. Over the past decade, bio-hybrid methods have been investigated as a promising new approach to overcome the challenges of scaling down robotic and other functional devices. These methods integrate biological cells with artificial components and therefore, can take advantage of the intrinsic actuation and sensing functionalities of biological cells. Here, the recent advancements in bio-hybrid actuation are reviewed, and the challenges associated with the design, fabrication, and control of bio-hybrid microsystems are discussed. As a case study, focus is put on the development of bacteria-driven microswimmers, which has been investigated as a targeted drug delivery carrier. Finally, a future outlook for the development of these systems is provided. The continued integration of biological and artificial components is envisioned to enable the performance of tasks at a smaller and smaller scale in the future, leading to the parallel and distributed operation of functional systems at the microscale.

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Section: Bio‐hybrid Methodsmentioning

confidence: 99%

Section: Bio‐hybrid Methodsmentioning

confidence: 99%

Section: Bio‐hybrid Methodsmentioning

confidence: 99%

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Bio‐Hybrid Cell‐Based Actuators for Microsystems

Carlsen

Sitti

2014

Small

198

199

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“…Electrical stimulation [16], [17], [22] and chemical stimulation [15], [23] are well-known to regulate muscle contractions. Recently, an optogenetic excitation control technique was reported that expressed a light-activated cation channel channelrhodopsin-2 on mouse cardiomyocytes [27] and on dorsal vessel of Drosophila melanogaster [28]. This technique has enabled us to regulate contractions of muscle tissue and cells with high temporal and spatial resolutions.…”

Section: Resultsmentioning

confidence: 99%

Room Temperature Operable Autonomously Moving Bio-Microrobot Powered by Insect Dorsal Vessel Tissue

et al. 2012

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Living muscle tissues and cells have been attracting attention as potential actuator candidates. In particular, insect dorsal vessel tissue (DVT) seems to be well suited for a bio-actuator since it is capable of contracting autonomously and the tissue itself and its cells are more environmentally robust under culturing conditions compared with mammalian tissues and cells. Here we demonstrate an autonomously moving polypod microrobot (PMR) powered by DVT excised from an inchworm. We fabricated a prototype of the PMR by assembling a whole DVT onto an inverted two-row micropillar array. The prototype moved autonomously at a velocity of 3.5×10−2 µm/s, and the contracting force of the whole DVT was calculated as 20 µN. Based on the results obtained by the prototype, we then designed and fabricated an actual PMR. We were able to increase the velocity significantly for the actual PMR which could move autonomously at a velocity of 3.5 µm/s. These results indicate that insect DVT has sufficient potential as the driving force for a bio-microrobot that can be utilized in microspaces.

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“…that chemical stimuli via crustacean cardioactive peptide could increase contractions of C. agnata DVT by 5.6-fold and may be superior to electrical and thermal methods which can be detrimental to the cells (Akiyama et al, 2008a). Yet another study successfully stimulated Drosophila melanogaster DVT via optogenetic engineering and light pulses of 2 Hz, suggesting optogenetics as promising low-cost and non-invasive control strategy (Suzumura et al, 2011).…”

Section: Bioactuatorsmentioning

confidence: 99%

Possibilities for Engineered Insect Tissue as a Food Source

Rubio

Fish

Trimmer

et al. 2019

Front. Sustain. Food Syst.

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Due to significant environmental concerns associated with industrial livestock farming, it is vital to accelerate the development of sustainable food production methods. Cellular agriculture may offer a more efficient production paradigm by using cell culture, as opposed to whole animals, to generate foods like meats, eggs, and dairy products. However, the cost-effective scale-up of cellular agriculture systems requires addressing key constraints in core research areas: (1) cell sources, (2) growth media, (3) scaffolding biomaterials, and (4) bioreactor design. Here we summarize work in the area of insect cell cultures as a promising avenue to address some of these needs. We also review current applications of insect cell culture and tissue engineering, provide an overview of insect myogenesis and discuss various properties of insect cells that indicate suitability for use in food production systems. Compared to mammalian or avian cultures, invertebrate cell cultures require fewer resources and are more resilient to changes in environmental conditions, as they can thrive in a wide range of temperature, pH and osmolarity conditions. Alterations necessary for large-scale production are relatively simple to achieve with insect cells, including immortalization, serum-free media adaptation and suspension culture. Additional benefits include ease of transfection, nutrient density, and relevance to seafood organisms. To advance insect-based tissue engineering for food purposes, it is necessary to develop methods to regulate the differentiation of insect cells into relevant cell types, characterize cell interactions with biomaterials with an eye toward 3D culture, design supportive bioreactor systems and quantify nutritional profiles of cultured biomass.

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A light-regulated bio-micro-actuator powered by transgenic Drosophila melanogaster muscle tissue

Cited by 14 publications

References 10 publications

Bio‐Hybrid Cell‐Based Actuators for Microsystems

Bio‐Hybrid Cell‐Based Actuators for Microsystems

Room Temperature Operable Autonomously Moving Bio-Microrobot Powered by Insect Dorsal Vessel Tissue

Possibilities for Engineered Insect Tissue as a Food Source

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