A light pipe for the transillumination of hollow organs during in vivo microscopy

Starr, Matthew C.; Netto, Daniel J.

doi:10.1016/0026-2862(73)90084-8

Cited by 1 publication

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“…An even higher numerical aperture might be obtained using a light pipe system with focusing lenses, e.g. something of the nature described by Starr & Netto (1973). Condensing lens systems at the tip of a light pipe are, however, more space demanding than the tapered prism.…”

Section: Discussionmentioning

confidence: 99%

Control of tissue environment during vital microscopy of the microcirculation in the m. tenuissimus in cat

Amundson¹,

Bagge²,

Haljamäe

1980

Acta Physiologica Scandinavica

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The physiological preservation of the tenuissimus muscle preparation in cat during vital microscopy of the microcirculation is assessed, comparing the originally described technique with a modified approach. Differences in the compared techniques include modes of dissection and transillumination, room-air exposure and moisturizing procedures. The original technique involves extensive dissection, inadequate temperature control and irrigation in open air. The modified technique involves less surgery due to a new illumination system, controlled heating and a Mylar foil cover on the preparation to minimize room-air influences. Temperature measurements and analyses of energy metabolism (ATP, CP, glucose, G6-P and lactate) are used as objective criteria of tissue normalcy. The microcirculation and metabolism are evaluated during anesthesia (alpha-chloralose) at rest as well as in hemorrhagic shock. In the resting state, muscle temperature drops to 28 degrees C with the irrigation technique, whereas the Mylar technique keeps the temperature at 34--35 degrees C. Neither technique causes deviations in normal metabolism. In shock, however, the temperature in the irrigated tenuissimus muscle fall 8--9 degrees C below deep muscle temperature and there is a significantly attenuated metabolic response to ischemia, while the Mylar preparation follows the changes of unexposed muscle, both in temperature and metabolism.

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Section: Discussionmentioning

confidence: 99%