A light-initiated chemiluminescent assay for rapid quantitation of allergen-specific IgG4 in clinical samples

Li, Junpu; Li, Shaoshen; Huang, Liying; Cui, Yaqiong; She, Tiantian; Bian, Ying; Li, Huiqiang

doi:10.1016/j.cca.2018.11.036

Cited by 12 publications

(12 citation statements)

References 37 publications

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“…A bead-based light-initiated chemiluminescent assay (LICA) system was demonstrated in our previous studies to determine serum egg component-sIgE and sIgG4 profiles. LICA has been proven to have excellent analytical performances and a good correlation with Phadia Immu-noCAP tests in sIgE measurement [16,17]. Yet, only a limited profile of allergy tests has been introduced in our country; LICA could be a reliable complement in allergy screening and diagnosis.…”

Section: Discussionmentioning

confidence: 99%

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[Retracted] Component‐Resolved Diagnostic Study of Egg Allergy in Northern Chinese Children

Zhang

Shen

et al. 2020

BioMed Research International

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Background. Egg component-specific IgE can be useful to evaluate and diagnose egg allergy, but their prevalence and clinical significance remain unclear in the local population. Previous studies have led to contradictory results regarding the value of specific IgG and specific IgG4 in sensitization. Objective. We aimed to determine the level of specific IgE, IgG, and IgG4 antibodies to the major egg allergens in egg-allergic children. Methods. Children from 6 months to 10 years of age were recruited. Egg allergy was confirmed by either a strong clinical history or an increased egg white-sIgE level. Other allergies were diagnosed by reactivity to other allergens but without egg-related symptoms and history. The serum sIgE, sIgG, and sIgG4 levels to major egg allergenic components (Gal d 1, Gal d 2, Gal d 3, Gal d 4, and Gal d 5), sIgE level to egg white, and tIgE level were determined by light-initiated chemiluminescent assay (LICA), ELISA, or ImmunoCAP. Results. Egg-allergic children had significantly higher levels of sIgE, sIgG, and sIgG4 to egg components than nonallergic children. Gal d 2 was the predominant allergen, and Gal d 2 sIgE level correlated with the egg white-sIgE level. Ratios of sIgE/sIgG4 to egg components were highest before 1 year of age and dropped gradually in the first decade of life. Conclusion. Patterns of sIgE to egg components could distinguish different forms of egg allergy. Ratios of sIgE/sIgG4 could be useful in predicting tolerance in egg-sensitive subjects, but this needs further evaluation and investigation using more accurate models.

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Section: Discussionmentioning

confidence: 99%

“…Samples with sIgE to egg white ≥ 0:35 kU A /L were defined as positive. [16,17]. The results of sIgE and sIgG4 were calculated as relative light units (RLU).…”

Section: Determination Of Total Ige and Egg-specific Ige Levelsmentioning

confidence: 99%

[Retracted] Component‐Resolved Diagnostic Study of Egg Allergy in Northern Chinese Children

Zhang

Shen

et al. 2020

BioMed Research International

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“…The levels of sige and sigG 4 to rPen a 1 as well as its peptides were measured using a light-initiated chemiluminescent assay (lica) carried out as previously described (34,35). lica is based on the formation of nanoparticle pairs and luminescence oxygen channeling immunoassay technology (36,37).…”

Section: Preparation Of Biotinylated Recombinant Pen a 1 Ez-linkmentioning

confidence: 99%

“…lica is based on the formation of nanoparticle pairs and luminescence oxygen channeling immunoassay technology (36,37). Studies have shown that this new method possesses excellent performance characteristics with high sensitivity, broad analytical range and rapid turnaround cycles and is suitable for immunodetection of allergen sige and sigG 4 (34,35 well and incubated at 37˚C for 30 min with mild agitation. Subsequently, 150 µl streptavidin-coated sensibeads (Beyond Biotech, co., ltd.) were added.…”

Section: Preparation Of Biotinylated Recombinant Pen a 1 Ez-linkmentioning

confidence: 99%

IgE and IgG4 responses to shrimp allergen tropomyosin and its epitopes in patients from coastal areas of northern China

Kong

et al. 2020

Mol Med Report

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Sensitization to allergens and their peptides varies among patients due to geographical or ethnic differences. The present study aimed to investigate immunoglobulin (ig)e and igG 4 responses to tropomyosin and its peptides in shrimp allergic patients from northern china. a total of 92 subjects were studied, including 35 shrimp allergic patients, 29 patients with house dust mite (HdM) and/or cockroach allergic patients and 28 healthy volunteers. Serum ige and igG 4 antibodies to recombinant shrimp tropomyosin (rPen a 1) and its peptides were measured by means of a light-initiated chemiluminescent assay. a total of 9 major sequential epitopes of Pen a 1 reported in the literature were synthesized. of 35 shrimp allergic patients, 25 (71.4%) had positive Pen 1-specific IgE (sige) antibodies and 22 (62.9%) contained measurable rPen a 1-specific igG 4 (sigG 4 ) antibodies. a strong igG 4 response accompanied the presence of ige to Pen a 1. none of the patients with HdM and/or cockroach allergy demonstrated ige reactivity to rPen a 1. The reaction frequency of ige binding epitope was 20-48%, while that of igG 4 binding epitope was 63.6-90.9%. The ige and igG 4 recognition patterns of the tropomyosin peptides demonstrated high interpatient heterogeneity. diversity of ige binding epitopes was positively correlated with Pen a 1 sige levels. in the study population, tropomyosin was a major allergen recognized by the majority of shrimp allergic patients, which is consistent with previous reports. However, none of the 9 epitopes are major (reaction frequency >50%) ige-binding regions, indicating the epitopes profile may be different in other regions.

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“…To the best of our knowledge, there has been no report on the LICA-based detection of inhaled allergen-sIgE till date [6][7][8][9][10][11][12][13]. Moreover, the Artemisia pollen is the most widespread outdoor allergen throughout China, which can cause allergic rhinitis, asthma, and/or both [14].…”

Section: Introductionmentioning

confidence: 99%

A Simple and Rapid Light-Initiated Chemiluminescence Assay for Quantitation of Artemisia-Specific Immunoglobulin E

Liu

Zhang

Zhu

et al. 2021

Int Arch Allergy Immunol

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Background: Light-initiated chemiluminescence assay (LICA) is a homogeneous assay that has been successfully used for the quantitation of food allergen-specific immunoglobulin E (sIgE), but not inhaled allergen-sIgE. Simultaneously, current assays used to detect allergen-sIgE are serum consuming and/or time consuming. Hence, we established a method for the quantitation of Artemisia-sIgE based on LICA and verified its performance according to the clinical guideline documents, laying a foundation for the quantitation of inhaled and food allergen-sIgE in parallel on LICA. Methods: The assay was established after optimizing the first incubation time and the dilutions of Artemisia-coated chemibeads, biotinylated goat anti-human IgE, and serum. In order to quantitate Artemisia-sIgE, the calibration curve was established with a high positive serum of known concentration. The assay performance was confirmed per the clinical guideline documents. In addition, the correlation between the results of LICA and capture enzyme-linked immunosorbent assay was evaluated. Results: The developed LICA’s coefficients of variation of repeatability and intermediate precision were 3.20%, 2.14%, and 3.85% and 4.30%, 4.00%, and 4.40%, respectively. The limit of detection was 0.10 kUA/L, and the limit of quantitation was 0.11 kUA/L. The range of linearity was from 0.27 kUA/L to 97.53 kUA/L (r = 0.9968). The correlation coefficient (r) for the correlation analysis between results of LICA and capture ELISA was 0.9087. This assay was successfully applied in 64 human serum samples, showing good sensitivity (82.20%) and specificity (100%). Conclusion: An Artemisia-sIgE quantitation assay based on LICA was successfully established. Its performance satisfied the clinical requirements and could be widely used in clinical laboratories.

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A light-initiated chemiluminescent assay for rapid quantitation of allergen-specific IgG4 in clinical samples

Cited by 12 publications

References 37 publications

[Retracted] Component‐Resolved Diagnostic Study of Egg Allergy in Northern Chinese Children

[Retracted] Component‐Resolved Diagnostic Study of Egg Allergy in Northern Chinese Children

IgE and IgG4 responses to shrimp allergen tropomyosin and its epitopes in patients from coastal areas of northern China

A Simple and Rapid Light-Initiated Chemiluminescence Assay for Quantitation of <b><i>Artemisia</i></b>-Specific Immunoglobulin E

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