A Light-Induced Tryptophan-Riboflavin Binding: Biological Implications

Silva, Eduardo; Salim-Hanna, Marta; Edwards, A.; Becker, María; Ioannes, Alfredo E. De

doi:10.1007/978-1-4899-2626-5_3

Cited by 19 publications

(12 citation statements)

References 65 publications

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“…This is interesting given that the increase in DCF-positive cells was not detected until day 5 following Mirasol treatment and storage, but this may be explained by the role of riboflavin as a photosensitizer, which may induce oxidation by multiple mechanisms [43]. Firstly, direct binding of riboflavin may occur, resulting in photoadduct formation and subsequent damage [44]. Alternatively, oxidative molecules, in addition to those measured in this study, may result in protein carbonylation [42].…”

Section: Discussionmentioning

confidence: 99%

Treatment of Platelet Concentrates with the Mirasol Pathogen Inactivation System Modulates Platelet Oxidative Stress and NF-κB Activation

Johnson¹,

Marks²

2015

Transfus Med Hemother

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Background: Pathogen inactivation (PI) technologies for platelets aim to improve transfusion safety by preventing the replication of contaminating pathogens. However, as a consequence of treatment, aspects of the platelet storage lesion are amplified. Mirasol treatment also affects platelet signal transduction and apoptotic protein expression. The aim of this study was to examine the effect of Mirasol treatment on the generation of reactive oxygen species (ROS) and subsequent oxidative stress. Methods: Pooled platelet concentrates were prepared in platelet-additive solution (70% SSP+ / 30% plasma). ABO-matched platelets were pooled and split, and treated with the Mirasol system (TerumoBCT) or left untreated as a control. Platelet samples were tested on day 1, 5, and 7 post-collection. Results: Mirasol-treated platelets had increased formation of ROS by day 5 of storage. Oxidative damage, in the form of protein carbonylation, was higher in Mirasol-treated platelets, whilst no effect on nitrotyrosine formation or lipid peroxidation was detected. The NF-κB signaling pathway was also activated in Mirasol-treated platelets, with increased expression and phosphorylation of NF-κB p65 and IκBa. Conclusion: These data demonstrate that Mirasol-treated platelets produce more ROS and display protein alterations consistent with oxidative damage.

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Section: Discussionmentioning

confidence: 99%

Treatment of Platelet Concentrates with the Mirasol Pathogen Inactivation System Modulates Platelet Oxidative Stress and NF-κB Activation

Johnson¹,

Marks²

2015

Transfus Med Hemother

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“…The deviation of the peak area for vitamin B 2 , which was quite stable at every previous storage conditions, is decreasing very fast in this case. The decomposition of riboflavin to peroxides and tryptophan derivatives due to light reaction is well known [29].…”

Section: Storage Stabilitymentioning

confidence: 99%

Simultaneous separation and analysis of water‐ and fat‐soluble vitamins on multi‐modal reversed‐phase weak anion exchange material by HPLC‐UV

Dabre

Azad

Schwämmle

et al. 2011

J of Separation Science

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Several methods for the separation of vitamins on HPLC columns were already validated in the last 20 years. However, most of the techniques focus on separating either fat- or water-soluble vitamins and only few methods are intended to separate lipophilic and hydrophilic vitamins simultaneously. A mixed-mode reversed-phase weak anion exchange (RP-WAX) stationary phase was developed in our laboratory in order to address such mixture of analytes with different chemical characteristics, which are difficult to separate on standard columns. The high versatility in usage of the RP-WAX chromatographic material allowed a baseline separation of ten vitamins within a single run, seven water-soluble and three fat-soluble, using three different chromatographic modes: some positively charged vitamins are eluted in ion exclusion and ion repulsion modes whereas the negatively charged molecules are eluted in the ion exchange mechanism. The non-charged molecules are eluted in a classical reversed-phase mode, regarding their polarities. The method was validated for the vitamin analysis in tablets, evaluating selectivity, robustness, linearity, accuracy, and precision. The validated method was finally employed for the analysis of the vitamin content of some commercially available supplement tablets.

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“…It is well known that riboflavin sodium phosphate produces a lumichrome in a neutral and acidic solution under light irradiation to yield fluorescence, 7) and a tryptophan-riboflavin complex is formed by oxidation reactions under light irradiation in an acidic alcohol solution. [8][9][10][11][12] These reports suggest the possibility that glucose, tryptophan, riboflavin, and insulin in the HPN preparation form a mixture which may contribute to the increase of the insulin peak area. As shown in Fig.…”

Section: Discussionmentioning

confidence: 99%

Apparent Increase of Insulin Peak Area in HPLC Analysis of a Preparation Consisting of a Mixture of Insulin and Total Parenteral Nutrition

Ichikawa

Kimura

Mori

et al. 2006

CHEMICAL & PHARMACEUTICAL BULLETIN

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A Light-Induced Tryptophan-Riboflavin Binding: Biological Implications

Cited by 19 publications

References 65 publications

Treatment of Platelet Concentrates with the Mirasol Pathogen Inactivation System Modulates Platelet Oxidative Stress and NF-κB Activation

Treatment of Platelet Concentrates with the Mirasol Pathogen Inactivation System Modulates Platelet Oxidative Stress and NF-κB Activation

Simultaneous separation and analysis of water‐ and fat‐soluble vitamins on multi‐modal reversed‐phase weak anion exchange material by HPLC‐UV

Apparent Increase of Insulin Peak Area in HPLC Analysis of a Preparation Consisting of a Mixture of Insulin and Total Parenteral Nutrition

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A Light-Induced Tryptophan-Riboflavin Binding: Biological Implications

Cited by 19 publications

References 65 publications

Treatment of Platelet Concentrates with the Mirasol Pathogen Inactivation System Modulates Platelet Oxidative Stress and NF-&#954;B Activation

Treatment of Platelet Concentrates with the Mirasol Pathogen Inactivation System Modulates Platelet Oxidative Stress and NF-&#954;B Activation

Simultaneous separation and analysis of water‐ and fat‐soluble vitamins on multi‐modal reversed‐phase weak anion exchange material by HPLC‐UV

Apparent Increase of Insulin Peak Area in HPLC Analysis of a Preparation Consisting of a Mixture of Insulin and Total Parenteral Nutrition

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Product

Resources

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Treatment of Platelet Concentrates with the Mirasol Pathogen Inactivation System Modulates Platelet Oxidative Stress and NF-κB Activation

Treatment of Platelet Concentrates with the Mirasol Pathogen Inactivation System Modulates Platelet Oxidative Stress and NF-κB Activation