A light-induced hydrogel responsive platform to capture and selectively isolate single circulating tumor cells

Chen, Bei; Wang, Ganggang; Huang, Chunyu; Sun, Yue; Zhang, Jing; Chai, Zhuomin; Guo, Shishang; Zhao, Xingzhong; Yua, Yuan; Liu, Wei

doi:10.1039/d1nr06876h

Cited by 7 publications

(3 citation statements)

References 44 publications

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“…Tumor metastasis is the primary reason for more than 90% of cancer-associated deaths. − In the process of tumor metastasis, circulating tumor cells (CTCs) are shed from the solid tumors and enter the bloodstream, eventually colonizing in distant tissues or organs and forming new metastatic foci. − CTCs have been regarded as important noninvasive biomarkers and hold great potential for early cancer diagnosis, prediction of recurrence, and individualized treatment. − However, the extremely rare CTCs are present in the complex whole blood, which contains only a few to a dozen CTCs per mL while billions of blood cells. − Some materials have been developed to improve the capture performance toward CTCs, while most of them cannot achieve fully stereo contacts with target cells. − Compared with the smooth plane, the three-dimensional (3D) micro-/nanostructure with the ability to interact with cells can significantly improve the capture efficiency of CTCs . In addition, the fragility of CTCs is another key issue that needs to be considered.…”

Section: Introductionmentioning

confidence: 99%

Tumor Microenvironment-Inspired Glutathione-Responsive Three-Dimensional Fibrous Network for Efficient Trapping and Gentle Release of Circulating Tumor Cells

Luo,

He,

et al. 2023

ACS Appl. Mater. Interfaces

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Detection of circulating tumor cells (CTCs) is important for early cancer diagnosis, prediction of postoperative recurrence, and individualized treatment. However, it is still challenging to achieve efficient capture and gentle release of CTCs from the complex peripheral blood due to their rarity and fragility. Herein, inspired by the three-dimensional (3D) network structure and high glutathione (GSH) level of the tumor microenvironment (TME), a 3D stereo (3D-G@FTP) fibrous network is developed by combining the liquid-assisted electrospinning method, gas foaming technique, and metal–polyphenol coordination interactions to achieve efficient trapping and gentle release of CTCs. Compared with the traditional 2D@FTP fibrous scaffold, the 3D-G@FTP fibrous network could achieve higher capture efficiency (90.4% vs 78.5%) toward cancer cells in a shorter time (30 min vs 90 min). This platform showed superior capture performance toward heterogeneous cancer cells (HepG2, HCT116, HeLa, and A549) in an epithelial cell adhesion molecule (EpCAM)-independent manner. In addition, the captured cells with high cell viability (>90.0%) could be gently released under biologically friendly GSH stimulus. More importantly, the 3D-G@FTP fibrous network could sensitively detect 4–19 CTCs from six kinds of cancer patients’ blood samples. We expect this TME-inspired 3D stereo fibrous network integrating efficient trapping, broad-spectrum recognition, and gentle release will promote the development of biomimetic devices for rare cell analysis.

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Section: Introductionmentioning

confidence: 99%

Tumor Microenvironment-Inspired Glutathione-Responsive Three-Dimensional Fibrous Network for Efficient Trapping and Gentle Release of Circulating Tumor Cells

Luo,

He,

et al. 2023

ACS Appl. Mater. Interfaces

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“…This research offers a promising start towards creating an affordable, light-responsive hydrogel for targeted tumour treatment. Chondroitin sulfate methacryloyl (CSMA) [122] and methylcellulose [125] like polymers are investigated for crafting light-induced hydrogels, pivotal in cancer treatment. These innovative hydrogels, responsive to light stimuli, adeptly encapsulate potent therapeutic agents like DOX, facilitating precise chemo-and photothermal therapy, thereby efficacy in combating cancer cells.…”

Section: Light Responsive Hydrogelsmentioning

confidence: 99%

Stimulus Responsive Hydrogels for Targeted Cancer Therapy

Solanki,

Bhatia

2024

Preprint

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Cancer is a highly heterogeneous disease and remains a global health challenge affecting millions of human lives worldwide. Despite advancements in conventional treatments like surgery, chemotherapy, and immunotherapy, the rise of multidrug resistance, tumor recurrence, and severe side effects necessitates innovative therapeutic approaches. The complex nature of the tumor microenvironment (TME) further compromises the efficacy of traditional chemotherapy drugs. Recently, stimulus-responsive nanomedicines designed to target TME characteristics (e.g., pH, redox, enzymes) have gained attention for their potential to enhance anticancer efficacy while minimizing adverse effects. Among various nanocarriers, hydrogels are an interesting class of nanocarriers used in cancer therapy due to their high water content, adjustable mechanical characteristics, and ability to respond to external/internal stimuli. These properties make hydrogels an ideal nanocarrier for controlled drug release within the TME. This review comprehensively surveys the latest advancements in stimulus-responsive hydrogels for cancer therapy, exploring various stimuli-responsive mechanisms, including biological (e.g., pH, redox), chemical (e.g., enzymes, glucose), and physical (e.g., temperature, light), as well as dual- or multi-stimuli responsiveness. This review will offer novel perspectives on the development of stimulus-responsive hydrogels for cancer therapy.

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“…Factors significantly influencing these analyses include cell viability, release efficiency, and specificity. Except potentially harmful methodologies such as the application of physical forces 114 and trypsinization, 115 suitable techniques for liberating CTCs from capture apparatus can also be organized into physical signal response (heat, light, and electric) and chemical signal response (pH, enzyme, and substrate breakdown).…”

Section: Advanced Technologies For Ctc Releasementioning

confidence: 99%

Recent advances of liquid biopsy: Interdisciplinary strategies toward clinical decision‐making

Bao,

Min,

et al. 2023

Interdisciplinary Medicine

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Liquid biopsy has emerged as a promising avenue for non‐invasive and rapid retrieval of pathological information from patient body fluids. Over the years, liquid biopsy has garnered significant attention for clinically treating cancer by selecting appropriate biomarkers such as circulating tumor cells (CTCs) and extracellular vesicles (EVs). Further integration of advanced technologies has facilitated the efficient capture of biomarkers for liquid biopsy, revolutionizing clinical decision‐making in the multiple processes and stages of cancer patients. Underscoring the intersection of different disciplines, this review provides a holistic summary of recent breakthroughs specifically designed for the capture and application of distinctive biomarkers to blend real‐world clinical decision‐making with material science. Firstly, we focus on the main principles of liquid biopsy and recent technologies that facilitate the capture and release of biomarkers (e.g., CTCs and EVs), leveraging their physicochemical properties. Then, the clinical applications of biomarkers are summarized, highlighting their potential for providing comprehensive clinical information. Later, the incorporation of machine learning is also emphasized for enhancing clinical applications and enabling deeper insights in the design of next‐generation platforms for specific biomarker isolation. Finally, future opportunities for clinical decision‐making are explored by combining advanced nanotechnologies and artificial intelligence, thereby offering inconceivable possibilities for improving patient care.

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A light-induced hydrogel responsive platform to capture and selectively isolate single circulating tumor cells

Abstract: Isolation of circulating tumor cells (CTCs) from patients is a challenge due to the rarity of CTCs. Recently, various platforms to capture and release CTCs for downstream analysis have been...

Cited by 7 publications

References 44 publications

Tumor Microenvironment-Inspired Glutathione-Responsive Three-Dimensional Fibrous Network for Efficient Trapping and Gentle Release of Circulating Tumor Cells

Tumor Microenvironment-Inspired Glutathione-Responsive Three-Dimensional Fibrous Network for Efficient Trapping and Gentle Release of Circulating Tumor Cells

Stimulus Responsive Hydrogels for Targeted Cancer Therapy

Recent advances of liquid biopsy: Interdisciplinary strategies toward clinical decision‐making

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