A light and electron microscopic study of alkaline phosphatase activity in the early stage of dentinogenesis in the young rat

Yoshiki, Shusaku; Kurahashi, Y.

doi:10.1016/0003-9969(71)90043-4

Cited by 80 publications

(20 citation statements)

References 16 publications

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“…The time dependent appearance and localization of alkaline phosphatase in bovine incisors resem bles the odontogenesis of ham ster and rat [56,57]. The previously reported presence of alkaline phos phatase in the odontoblasts during matrix form a tion [58] could not be confirmed by our study, we think that the former finding is due to diffusion ar tifacts resulting from the very active subodonto blasts.…”

Section: A Alkaline Phosphatasecontrasting

confidence: 56%

Phosphohydrolase Activities in Developing and Mature Dental Tissues

Warth

Jeck

1990

Zeitschrift Für Naturforschung C

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Phosphohydrolases, Dental Tissues, Tooth D evelopm ent, MineralizationIn the course o f the odontogenesis o f bovine incisors several clearly distinguishable phos phohydrolase activities are observed in the pulp and in dental hard tissues. Using various sub strates and inhibitors, unspecific alkaline phosphatase, two isoenzymes o f acid phosphatase, Ca2+-activated ATPase and inorganic pyrophosphatase are characterized. The enzymatic ac tivity o f alkaline phosphatase in pulp and hard tissues is significantly high at the beginning o f dentine and enamel mineralization. The specific activity o f this enzyme decreases quite fast with the beginning o f root form ation, then more slowly, until it reaches a constant final value. Histochemical studies show that during mineralization the maximum o f alkaline phosphatase activity is in the subodontoblasts. Lower enzyme concentrations are found in the stratum intermedium and in the outer enamel epithelium during that process.The specific activities o f ATPase, acid phosphatases and pyrophosphatase show little tem poral variation during tooth developm ent, but they also appear in a characteristic spatial pattern in the dental tissues.

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Section: A Alkaline Phosphatasecontrasting

confidence: 56%

Phosphohydrolase Activities in Developing and Mature Dental Tissues

Warth

Jeck

1990

Zeitschrift Für Naturforschung C

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“…Moreover, when combined with SMF exposure, Dex/b-GP decreased cell proliferation as early as at 7 days, together with a higher expression of ALP activity. The expression of ALP activity is essential and an early indicator of osteoblastic differentiation in mesenchymal stem cells (Yoshiki and Kurahashi 1971;Osyczka et al 2004). SMF exposure alone did not induce ALP activity, extracellular calcium concentration, or affect cell proliferation, but it promoted the osteogenic differentiation induced by Dex/b-GP, including the expression of osteogenic genes, as well as the increase in ALP activity and calcium accumulation.…”

Section: Resultsmentioning

confidence: 92%

The static magnetic field accelerates the osteogenic differentiation and mineralization of dental pulp cells

Hsu

Chang

2010

Cytotechnology

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Dental pulp cells (DPCs) can differentiate into osteoblasts and are deemed a promising cell source for bone regeneration. Static magnetic field (SMF) stimulates osteoblast differentiation but the effect in DPCs remains unknown. The aim of this study was to investigate the effect of SMF exposure on the osteogenic differentiation and mineralization of rat DPCs in vitro. Cells were continuously exposed to SMF at 290 mT in the presence/absence of osteogenic induction [dexamethasone (Dex)/b-glycerophosphate (b-GP)]. Results showed that SMF alone did not impair the cell cycle and proliferation. On the other hand, obvious condensation in the metachromatic staining of the extracellular matrix with toluidine blue was observed for SMF-exposed cells as well as the Dex/b-GP treated cells. SMF in combination with Dex/b-GP significantly increased the mRNA expression of osteogenic genes, as well as the ALP activity and extracellular calcium concentration at the early stage, followed by obvious calcium deposits later. Besides, SMF exposure increased the activity of extracellular signal-regulated kinase 1/2 (ERK1/2) at 3 h and accelerated the mRNA expression of osteogenic transcription factor, Cbfa1, advancing its activation time from 168 to 72 h under osteogenic induction. In summary, SMF exposure in combination of Dex/b-GP induction could significantly accelerate the osteogenic differentiation and mineralization of DPCs.

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“…It has been reported that the ALPase activity in hard tissue-forming cells, including pulp cells, is stronger at a greater distance from the mineralizing front (Kurahashi et al, 1969;Yoshiki and Kurahashi, 1971;Takahashi, 1972;Ozawa et al, 1979). Although there are various hypotheses, the biological significance of ALPase remains to be clarified.…”

Section: Discussionmentioning

confidence: 96%

Calcification capacity of dental papilla mesenchymal cells transplanted in the isogenic mouse spleen

1990

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The capacity of the dental pulp to form calcified tissue was examined in papilla cells dissociated from first molar tooth germs of the neonatal mouse and isografted in the spleen for up to 7 days. To obtain papilla cell populations without odontoblasts, pulpal mesenchyme was isolated mechanically from the enamel organ after 0.1% trypsin treatment and rolled on a membrane filter. On day 3 after transplantation, the grafted papilla cells had changed into large, spindle-shaped cells, and initial calcification with needle-like crystals began in association with the collagenous matrix surrounding those cells. On day 7 after transplantation, the spindle cells transformed into odontoblast-like cells containing well-developed secretory organelles, and irregular, but nontubular, calcified tissues were commonly observed surrounding the extracellular collagenous matrix. The calcified tissue matrix with cellular inclusions displayed a structure similar to that of osteodentin. During this period, an intense positive reaction for alkaline phosphatase (ALPase) activity was demonstrated along the cell membranes of the odontoblast-like cells aligned at the periphery of forming calcified tissue. Enzymatic activity could not be detected on the cells incorporated completely into osteodentin-like matrix. The present results show that the papilla cell population transplanted into the spleen formed osteodentin-like material, thus demonstrating the capacity of papilla cells to produce calcified tissue.

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A light and electron microscopic study of alkaline phosphatase activity in the early stage of dentinogenesis in the young rat

Cited by 80 publications

References 16 publications

Phosphohydrolase Activities in Developing and Mature Dental Tissues

Phosphohydrolase Activities in Developing and Mature Dental Tissues

The static magnetic field accelerates the osteogenic differentiation and mineralization of dental pulp cells

Calcification capacity of dental papilla mesenchymal cells transplanted in the isogenic mouse spleen

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