A Light and Electron Microscopic Study of Duodenal Epithelium of Chick Embryos Cultured in the Presence and Absence of Hydrocortisone

Dobbins, William O.; Hijmans, Jacqueline C.; McCarty, Kenneth S.

doi:10.1016/s0016-5085(19)34180-0

Cited by 29 publications

(11 citation statements)

References 30 publications

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“…In the present study proliferation was stimulated in the 20 day embryonic chick duodenal epithelium during organ culture for 24 hr with HC, as indicated by increased DNA synthesis ( 3 H-thymidine labeling), increased cell number/villus, and increased mitotic index after colchicine treatment. This agrees with increases in mitotic index found in 14 and 16 day chick embryo duodenum cultured for 3 to 4 days (Dobbins, Hijmans and McCarty 1967;Hijmans and McCarty 1969;Black 1978). The fact that neither total DNA nor total protein concentrations was altered suggests that HC did indeed increase proliferative activity.…”

Section: Discussionsupporting

confidence: 85%

“…Organ culture of the embryonic chick duodenum has been used to study development and function of the vitamin Ddependent calcium absorptive mechanism (Corradino 1973a,b;1979a,b;Parkes and Reynolds 1977;Peterlik 1978) and to study control of duodenal development by glucocorticoid and thyroid hormones (Moog and Nehari 1954;Moog and Kirsch 1955;Hayes 1965a,b;Hijmans and McCarty 1966;McCarty, Hijmans and Wilhelm 1966;Dobbins, Hijmans and McCarty 1967;Hijmans 1972;Yoshizawa et al 1976;\911;Black and Moog 1977;Black 1978;Black, Yoneyama and Moog 1980). Using this system, a bioassay has been developed for glucocorticoid activity based on promotion of villus lengthening (Pedernera 1970).…”

Section: Introductionmentioning

confidence: 99%

“…L.M. Brewer and R.A. Corradino Several studies have shown that HC affects mitotic activity of the chick embryo's duodenal epithelium in vitro (Dobbins, Hijmans and McCarty 1967 ;Hijmans and McCarty 1969;Hijmans 1972;Black 1978). Vitamin D 3 has been found to stimulate proliferation in the duodenal epithelium of rachitic clucks (Spielvogel, Farley and Norman 1972) and rats (Birge and Alpers 1973), but its effect on proliferation of the embryonic chick duodenum in organ culture has not been studied in detail.…”

Section: Introductionmentioning

confidence: 99%

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Hydrocortisone and 1,25(OH)₂D₃: Role in Proliferative Responses of the Embryonic Chick Duodenum in Organ Culture

Lm¹,

Ra²

1983

Horm Metab Res

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The present studies were conducted to determine if hydrocortisone (HC) and/or 1,25-dihydroxycholecalciferol (1,25(OH)2D3) alter proliferative responses of the cultured duodenum, and if such alterations could be related to the known augmentation of 1,25(OH)2D3-induction of a specific, intestinal calcium-binding protein (CaBP) by glucocorticoids. HC stimulated proliferation in the duodenal epithelium, as indicated by increased DNA synthesis (3H-thymidine uptake), increased cell number/villus, and increased mitotic index after colchicine treatment. Goblet cell numbers were not significantly increased with any hormone treatment. The presence of 1,25(OH)2D3 alone did not affect proliferative responses. CaBP concentration as a function of tissue weight was 50% greater in HC stimulated intestine, indicating that the proliferogenic action of HC on the duodenum alone could not account for the glucocorticoid-1,25(OH)2D3 interaction in CaBP synthesis.

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Section: Discussionsupporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Hydrocortisone and 1,25(OH)₂D₃: Role in Proliferative Responses of the Embryonic Chick Duodenum in Organ Culture

Lm¹,

Ra²

1983

Horm Metab Res

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“…2). During development the nuclei assumed a basal position, the apical cytoplasm enlarged showing a pale-staining region above the nucleus and depletion of lipid droplets (Dobbins et al 1967;Holman 1969). Goblet cells appeared most often at stage 45.…”

Section: Light Microscopymentioning

confidence: 99%

“…bins et al 1967; Black andMoog 1977, 1978; Black The ultrastructural differentiation of chicken embryo duodenal epithelium during the week before hatching has been described (Dobbins et al 1967;Holman 1969; Penttila and Gripenberg 1969). Differentiation of the brush border proceeds rapidly during this period (Moog 1950; Overton and Shoup 1964;Hugon and Borgers 1969;Ono 1973); these differentiative changes are believed to be under adenohypophyseal control (Hinni and Watterson 1963: Bellware and Betz 1970: Hart and 1978Brewer and Betz 1979;~orradino 1979).…”

Section: Introductionmentioning

confidence: 99%

Thyroxine treatments in vivo and ultrastructural differentiation of chicken (Gallus gallus) embryo duodenum

Brewer¹,

Betz²

1982

Can. J. Zool.

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BREWER, L. M., and T. W. BETZ. 1982. Thyroxine treatments in vivo and ultrastructural differentiation of chicken (Gallus gallus) embryo duodenum. Can. J. Zool. 60: 676-686.Previously, L-thyroxine (L-T4) treatment of 16.5-day-old chicken embryos (approximately stage 42) correlated with a precocious increase in prehatching duodenal alkaline phosphatase specific activity. This paper examines the effects of similar L-T4 treatment on ultrastructural differentiation of the absorptive epithelium. White Leghorn embryos (strain Hyline 934F of Gallus gallus) were given 0.1 mL of saline containing 5-pg doses of either L -T~, or D-T~, or saline vehicle alone through windows in the shell. Untreated windowed and intact unwindowed embryos were additional controls. At the time of treatment (16.5 days) and 24,48, and 72 h later embryos were killed and their duodena were fixed. At least three embryos were examined in each group. Twenty-four hours after treatment stage 43 epithelial cells resembled stage 42 cells. Forty-eight and 72 h following L-T4 treatment stage 44 and 45 embryos showed evidence of increased Golgi and lysosome activity but depletion of lipid droplets and glycogen stores. Control treatments with D -T~ or saline vehicle did not appear to affect epithelial cell ultrastructure as these embryos resembled unwindowed and windpwed control embryos at all stages. BREWER, L. M., et T. W. BETZ. 1982. Thyroxine treatments in vivo and ultrastructural differentiation of chicken ( Gallus gallus) embryo duodenum. Can. J. Zool. 60: 676-686. I1 est prouvC qu'un traitement la L-thyroxine (L-T4) administrC a des embryons de poulet de 16,5 jours (stade 42 environ) entraine une augmentation prCcoce de I'activitC spkcifique de la phosphatase alcaline du duodenum avant I'Cclosion. On examine cette fois les effets d'un traitement a la L-T4 sur la differenciation de l'ultra-structure de 1'CpithClium absorbant. Des embryons de la Leghorn blanche (souche Hyline 934 F de Gallus gallus) ont r e p soit 0,l mL de solution saline contenant des doses de 5 pg de L-T4 ou de D-T~, soit la solution saline seulement, administrC par des fenCtres dans la coquille. Des embryons a coquille trouCe et d'autres A coquille intacte ont aussi semi de tCmoins. Des embryons ont kt6 tuCs et leur duodCnum fix6 au moment du traitement (16,5 jours), d'autres 24, 48 et 72 h aprks le traitement. Au moins trois embryons ont Ct C examinks dans chaque groupe. Vingt-quatre heures aprks le traitement, les cellules CpithCliales de stade 43 ressemblent a celles de stage 42. Quarante-huit heures et 72 h aprh le traitement a la L -T~, les embryons de stade 44 et 45 portent des traces d'une activitC accrue des appareils de Golgi et des lysosomes, mais les gouttelettes de lipide et les rksemes de glycoghe ont diminuk.Chez les embryons traitCs a la D-T4 ou a la solution saline seulement, l'ultra-structure des cellules CpithCliales n'est pas affectke puisque ces cellules restent semblables a celles des embryons tCmoins de tous stades a coquille trouCe ou intacte.[Traduit par le jou...

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Morphological development of the epithelium of the embryonic chick intestine in culture: Influence of thyroxine and hydrocortisone

Black

1978

Am. J. Anat.

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A culture system with defined medium has been utilized to elucidate the role of thyroxine (T4) and hydrocortisone (HC) in growth and differentiation of the epithelium of embryonic chick duodenum during the third week of development. Duodenal explants maintained a basically normal morphology for 48 to 72 hours, but during subsequent culture, muscle and mesenchyme deteriorated, previllous ridges shrank, the epithelial surface became pitted, and the tissue lost weight. These degenerative processes were retarded by addition of HC to the culture medium. Previllous ridges of cultured duodenum failed to develop into true villi and ridge growth was subnormal. Epithelial mitotic counts were increased by T4 or HC, as compared to cultured control tissue, but dropped below values in vivo, by 72 hours in culture. Epithelial differentiation proceeded more rapidly in duodenum cultured without hormones than in vivo. Increase in cell height, flattening of the epithelial surface, attainment of uniform staining of the brush border with periodic acid-Schiff, increase in microvillar density, and formation of a terminal web were accelerated by 24 to 48 hours in culture. In the presence of T4, cell height and microvillar growth were stimulated further, reaching the state found at 19 to 21 days in vivo.

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A Light and Electron Microscopic Study of Duodenal Epithelium of Chick Embryos Cultured in the Presence and Absence of Hydrocortisone

Cited by 29 publications

References 30 publications

Hydrocortisone and 1,25(OH)₂D₃: Role in Proliferative Responses of the Embryonic Chick Duodenum in Organ Culture

Hydrocortisone and 1,25(OH)₂D₃: Role in Proliferative Responses of the Embryonic Chick Duodenum in Organ Culture

Thyroxine treatments in vivo and ultrastructural differentiation of chicken (Gallus gallus) embryo duodenum

Morphological development of the epithelium of the embryonic chick intestine in culture: Influence of thyroxine and hydrocortisone

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A Light and Electron Microscopic Study of Duodenal Epithelium of Chick Embryos Cultured in the Presence and Absence of Hydrocortisone

Cited by 29 publications

References 30 publications

Hydrocortisone and 1,25(OH)2D3: Role in Proliferative Responses of the Embryonic Chick Duodenum in Organ Culture

Hydrocortisone and 1,25(OH)2D3: Role in Proliferative Responses of the Embryonic Chick Duodenum in Organ Culture

Thyroxine treatments in vivo and ultrastructural differentiation of chicken (Gallus gallus) embryo duodenum

Morphological development of the epithelium of the embryonic chick intestine in culture: Influence of thyroxine and hydrocortisone

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About

Hydrocortisone and 1,25(OH)₂D₃: Role in Proliferative Responses of the Embryonic Chick Duodenum in Organ Culture

Hydrocortisone and 1,25(OH)₂D₃: Role in Proliferative Responses of the Embryonic Chick Duodenum in Organ Culture