A library of reporters of the global regulators of gene expression ofEscherichia coli
Suchintak Dash,
Rahul Jagadeesan,
Ines S.C. Baptista
et al.
Abstract:SUMMARYThe topology of the transcription factor network (TFN) ofE. coliis far from uniform, with 22 global regulator (GR) proteins controlling one-third of all genes. So far, their production rates cannot be tracked by comparable fluorescent proteins. We developed a library of fluorescent reporters for 16 GRs for this purpose. Each consists of a single-copy plasmid coding for GFP fused to the full-length copy of the native promoter. We tracked their activity in exponential and stationary growth, as well as und… Show more
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