A Less Invasive Approach of Medial Meniscectomy in Rat: A Model to Target Early or Less Severe Human Osteoarthritis

Juneja, Subhash C.; Ventura, Manuela; Jay, Gregory D.; Veillette, Christian

doi:10.4172/2167-7921.1000193

Cited by 7 publications

(6 citation statements)

References 66 publications

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“…Seven-week-old Slc:Wistar male rats were housed under a 12-h light cycle in a temperature-controlled room for 1 week before surgery was performed. Experimental OA was induced by MMx 28 . Briefly, anesthesia was induced in rats [medetomidine (2 mg/kg bodyweight), midazolam (0.4 mg/kg), butorphanol (5 mg/kg)].…”

Section: Methodsmentioning

confidence: 99%

Knockdown of mechanosensitive adaptor Hic-5 ameliorates post-traumatic osteoarthritis in rats through repression of MMP-13

Miyauchi

Noguchi

Lei

et al. 2023

Sci Rep

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Osteoarthritis (OA) is the most common joint disease associated with articular cartilage destruction. Matrix metalloproteinase-13 (MMP-13) has an essential role in OA pathogenesis by degradation of collagen II, a major component of articular cartilage. Hydrogen peroxide-inducible clone-5 (Hic-5; TGFB1I1), a transforming growth factor-β-inducible mechanosensor, has previously been reported to promote OA pathogenesis by upregulating MMP-13 expression in mouse osteoarthritic lesions. In our current study, immunohistochemical analysis showed that Hic-5 protein expression was increased in human OA cartilage compared with normal cartilage. Functional experiments demonstrated that Hic-5 and MMP-13 expression was increased by mechanical stress, and mechanical stress-induced MMP-13 expression was suppressed by Hic-5 siRNA in human chondrocytes. Moreover, intracellular localization of Hic-5 shifted to the nucleus from focal adhesions in human chondrocytes subjected to mechanical stress, and nuclear Hic-5 increased MMP-13 gene expression. In vivo, intra-articular injection of Hic-5 siRNA decreased the Osteoarthritis Research Society International score and MMP-13 protein expression in articular cartilage of OA rats. Our findings suggest that Hic-5 regulates transcription of MMP-13 in human chondrocytes, and Hic-5 may be a novel therapeutic target for OA because OA progression was suppressed by intra-articular injection of Hic-5 siRNA in rats.

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Section: Methodsmentioning

confidence: 99%

Knockdown of mechanosensitive adaptor Hic-5 ameliorates post-traumatic osteoarthritis in rats through repression of MMP-13

Miyauchi

Noguchi

Lei

et al. 2023

Sci Rep

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“…Even though chitosan shares structural similarities with the glucosamine glycans (GAGs) present in PGs, which have been shown to stimulate chondrogenesis [ 34 ], chitosan seems to interact with growth factors to stimulate fibrocytes and no other cells [ 35 , 36 ]. This may explain why despite an increase in the viability of the cells, they had a morphology like that of fibrocytes [ 22 ].…”

Section: Discussionmentioning

confidence: 99%

“…The presence of type II collagen (Col2A1) is related to the presence of healthy tissue, which, when hypertrophied after damage, begins to be replaced by type X collagen. On the other hand, metalloproteinase 13 (MMP- 13) is the most widely used marker of inflammation in the evaluation of cartilage, as it is the main protease in articular cartilage degradation [ 21 , 22 ].…”

Section: Methodsmentioning

confidence: 99%

Study of the Early Effects of Chitosan Nanoparticles with Glutathione in Rats with Osteoarthrosis

Ramírez-Noguera,

Zetina Marín,

Gómez Chavarin

et al. 2023

Pharmaceutics

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Due to cartilage’s limited capacity for regeneration, numerous studies have been conducted to find new drugs that modify osteoarthrosis’s progression. Some evidence showed the capability of chitosan nanoparticles with glutathione (Np-GSH) to regulate the oxide-redox status in vitro in human chondrocytes. This work aimed to evaluate the capacity of Np-GSH in vivo, using Wistar rats with induced surgical osteoarthritis. Radiographic, biochemical (GSH and TBARS quantification), histopathological, and immunohistochemical (Col-2 and MMP-13) analyses were performed to evaluate the progress of the osteoarthritic lesions after the administration of a single dose of Np-GSH. According to the results obtained, the GSH contained in the NPs could be vectored to chondrocytes and used by the cell to modulate the oxidative state reduction, decreasing the production of ROS and free radicals induced by agents oxidizing xenobiotics, increasing GSH levels, as well as the activity of GPx, and decreasing lipid peroxidation. These results are significant since the synthesis of GSH develops exclusively in the cell cytoplasm, and its quantity under an oxidation–reduction imbalance may be defective. Therefore, the results allow us to consider these nanostructures as a helpful study tool to reduce the damage associated with oxidative stress in various diseases such as osteoarthritis.

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“…Additionally, the hypertrophic condition also was observed OA condition as shown in Figure 5 . During progression of OA, chondrocytes convert to hypertrophic condition and disrupted arrangement of the chondrocytes [ 52 ]. This condition also elevates inflammation progression [ 53 ].…”

Section: Discussionmentioning

confidence: 99%

Seahorse Protein Hydrolysate Ameliorates Proinflammatory Mediators and Cartilage Degradation on Posttraumatic Osteoarthritis with an Obesity Rat Model

Sudirman

Tseng

Chen

et al. 2022

BioMed Research International

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Osteoarthritis (OA) is one of the age-related diseases and is highly present on the knees. Obesity and mechanical injuries as a risk factor of OA are attributed to cartilage disintegration, joint loading, and inflammation. This study is aimed at investigating the effects of seahorse protein hydrolysate (SH) on posttraumatic osteoarthritis in an obesity rat. The OA model was developed by anterior cruciate ligament transection with medial meniscectomy in a high-fat diet- (HFD-) induced obesity rat model. The male Sprague-Dawley rats were fed a HFD for 6 weeks before OA surgery. The OA rats were treated with oral gavage by 4, 8, or 20 mg/kg of body weight of SH for 6 weeks of treatment. The expressions of plasma proinflammatory factors, C-telopeptide of type II collagen, and matrix metalloproteinase- (MMP-) 3 and MMP-13 were reduced by SH treatment. Plasma superoxide dismutase and glutathione peroxidase activities were enhanced by SH. SH also relieved the pain of the knee joint and swelling as well as decreased proteoglycan loss in the knee articular cartilage caused by osteoarthritis. Based on these results, SH suppressed proinflammatory factors and attenuated cartilage degradation and pain in the OA model. Therefore, seahorse protein hydrolysate might be a potential opportunity for improving the development of osteoarthritis.

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A Less Invasive Approach of Medial Meniscectomy in Rat: A Model to Target Early or Less Severe Human Osteoarthritis

Cited by 7 publications

References 66 publications

Knockdown of mechanosensitive adaptor Hic-5 ameliorates post-traumatic osteoarthritis in rats through repression of MMP-13

Knockdown of mechanosensitive adaptor Hic-5 ameliorates post-traumatic osteoarthritis in rats through repression of MMP-13

Study of the Early Effects of Chitosan Nanoparticles with Glutathione in Rats with Osteoarthrosis

Seahorse Protein Hydrolysate Ameliorates Proinflammatory Mediators and Cartilage Degradation on Posttraumatic Osteoarthritis with an Obesity Rat Model

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