A leishmaniosis surveillance system among stray dogs in the region of Madrid: ten years of serodiagnosis (1996–2006)

Miró, Guadalupe; Montoya, Ana; Mateo, Marta; Alonso, Ana; Garcia, Sandra; García, A.; Caballero, M. J.; Molina, Ricardo

doi:10.1007/s00436-007-0497-8

Cited by 44 publications

(50 citation statements)

References 22 publications

(23 reference statements)

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“…They reported that they had not recorded any increase in the leishmaniasis detection tests performed in their clinics, where the prevalence of canine leishmaniasis was around 5%. In 2011, they performed leishmaniasis detection tests on 1,007 dogs during an anti-rabies vaccination campaign, using the rK39 blood test (BLK Fast Test, LETI), giving a prevalence of 1.0% in dogs that were household pets and 3.6% in dogs that were in dog pounds, results that were similar to those estimated in other studies performed in the region of Madrid [12,13].…”

Section: Monitoring Of Dogs the Main Known Reservoirsupporting

confidence: 67%

“…Given the role that dogs classically play as the reservoir, actions initially concentrated on their study, but the surveillance system did not detect any increase in the prevalence of leishmaniasis in these animals, with level being around 5% [11][12][13].…”

Section: Discussionmentioning

confidence: 99%

“…In Spain, dogs are considered to be the main reservoir for L. infantum [1][2][3][4][5][11][12][13]; in this outbreak, only 26% of cases acknowledged contact with dogs in their domestic or peridomestic environment and the cases with dogs as pets in their homes had already applied suitable methods to protect against sandfly bites [3,24]. In order to evaluate the possible presence of vectors, we asked patients about their environment (house, neighbourhood, work, leisure pursuits and holidays).…”

Section: Discussionmentioning

confidence: 99%

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Re-emergence of leishmaniasis in Spain: community outbreak in Madrid, Spain, 2009 to 2012

et al. 2013

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Since July 2009, there has been a community outbreak of leishmaniasis in the south-west area of the Madrid autonomous community, Spain, affecting residents from four towns that are geographically close together and share extensive park areas. As of December 2012, 446 cases were reported (6 in 2009, 97 in 2010, 196 in 2011 and 147 in 2012), a mean incidence rate of 22.2 per 100,000 inhabitants during July 2009 and December 2012. The mean age was 44 years (range: 2 months to 95 years); 61.0% were male. A total of 68 (15.2%) had immunosuppressive conditions; 160 (35.9%) had visceral leishmaniasis and 286 (64.1%) cutaneous. A total of 421 (94.4%) cases were confirmed. Leishmania infantum was identified as the agent. Monitoring revealed high densities of the vector Phlebotomus perniciosus. The surveillance system for canine leishmaniasis did not detect any increase in prevalence during the period. Environmental control measures have been taken, such as improvements in sanitation and disinsection in the risk areas and control of the overpopulation of Leporidae, as xenodiagnosis studies have shown that hares play a role as active reservoirs. This is the largest reported community outbreak of leishmaniasis in Europe. The discovery of the new reservoir stands out in the multifactorial aetiology of the outbreak. Epidemiological research and environmental intervention measures are continuing.

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Section: Monitoring Of Dogs the Main Known Reservoirsupporting

confidence: 67%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Re-emergence of leishmaniasis in Spain: community outbreak in Madrid, Spain, 2009 to 2012

et al. 2013

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“…Leishmania from dogs to humans, it is necessary to diagnose canine leishmaniasis as early as possible (35).…”

mentioning

confidence: 99%

Specific Serodiagnosis of Canine Visceral Leishmaniasis Using Leishmania Species Ribosomal Protein Extracts

Coelho

Ramírez

Costa³

et al. 2009

Clin Vaccine Immunol

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In the present work, we have analyzed the antigenicity of Leishmania species ribosomal proteins (LRPs). To accomplish this, Leishmania infantum ribosomes were biochemically purified from promastigote cytosolic extracts, and their reactivities were analyzed by using the sera from dogs naturally infected with L. infantum. Since antibodies reacting against different ribosomal proteins were observed in all the serum samples obtained from dogs with symptomatic visceral leishmaniasis tested, we have analyzed the potential usefulness of the LRP extracts in the development of an enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of canine visceral leishmaniasis (CVL) in an area of Brazil where visceral leishmaniasis is endemic due to infection by Leishmania chagasi. A comparative ELISA with crude soluble Leishmania chagasi antigen (SLA) and L. infantum LRPs was performed. LRP-and SLA-based ELISAs gave similar sensitivities for the diagnosis of symptomatic CVL, but the LRP extract provided a very high sensitivity for the detection of oligosymptomatic and asymptomatic dogs. In addition, an LRP-based ELISA showed a higher specificity when the sera from dogs harboring other infections were included in the analysis. The LRP antigen displayed no cross-reactivity with sera from dogs that had any of the other diseases tested, notably, Chagas' disease. Our findings suggest that LRPs are a potential tool for the diagnosis of CVL and will be particularly useful for the diagnosis of asymptomatic CVL.

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“…In a studies in southern Spain, the prevalences were reported as 5.3% (Acedo-Sánchez et al, 1996) and 22% in dogs older than 1 year old (Alonso et al, 2010), whereas, in Catalonia, the seroprevalence was reported as 10.2% (Fisa et al, 1999). The seroprevalence in Madrid region found in 1,803 dogs analysed was 7.8% (Miró et al, 2007) and 8.1 % in another study in the same region (Gálvez et al, 2010). However, the seroprevalence was lower (3.7%) in the northwestern Spain (Amusategui et al, 2004).…”

Section: Discussionmentioning

confidence: 95%

Prevalence of leishmaniosis in dogs in Istanbul,Turkey determined by using PCR

Bilgin

Turan

Yılmaz

et al. 2018

J Hellenic Vet Med Soc

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ABSTRACT. Leishmania infantum is widespread in Mediterranean countries including Turkey and can cause a serious disease in both humans and dogs. Dogs are considered to be its main reservoirs. The current epidemiological study was carried out in Istanbul for detection of leishmaniosis among dogs. A total of 246 dogs were included in the study. Twenty one (8.54%) blood samples were found to be positive based on PCR diagnosis, using primers specific for the kinetoplast DNA of Leishmania. Infection rate was 6.51% in 169 dogs living in shelters and 12.99% in 77 client-owned dogs. The rate was significantly (p<0.001) higher in the dogs (37.93%) presenting one or more clinical symptoms which may be attributable to leishmaniosis, than in the asymptomatic dogs (4.61%). Eleven (52.4%) of 21 PCR positive dogs presented clinical symptoms whereas the rest of the dogs (47.62%) were asymptomatic. The major clinical sign in PCR positive animals was dermatological problems. Amongst PCR positive dogs, skin lesions were present in 11, weight loss in 2, lymphadenopathy in 2, epistaxis in 2 and lethargy in 2 dogs.

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A leishmaniosis surveillance system among stray dogs in the region of Madrid: ten years of serodiagnosis (1996–2006)

Cited by 44 publications

References 22 publications

Re-emergence of leishmaniasis in Spain: community outbreak in Madrid, Spain, 2009 to 2012

Re-emergence of leishmaniasis in Spain: community outbreak in Madrid, Spain, 2009 to 2012

Specific Serodiagnosis of Canine Visceral Leishmaniasis Using Leishmania Species Ribosomal Protein Extracts

Prevalence of leishmaniosis in dogs in Istanbul,Turkey determined by using PCR

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