A study of modern genetics requires students to successfully unite the principles of Mendelian genetics with the functions of DNA. Traditional means of teaching genetics are often successful in teaching Mendelian and molecular ideas but not in allowing students to see how the two subjects relate. The laboratory module presented here attempts to present classical and molecular genetic concepts together as an inquiry-based exploration appropriate for high school or introductory undergraduate students. Using the non-essential APQ12 gene in the budding yeast Saccharomyces cerevisiae, students perform PCR, selective growth, and sporulation experiments to establish the ploidy and APQ12 zygosity of a series of unknown strains. Each experiment contributes data to characterize the unknown strains, but complete characterization is not possible without assimilating the data from all of the experiments. The module allows students to consider concepts normally introduced and emphasized in Mendelian genetics and explore them using molecular and experimental tools. Comparison of pre-module and post-module assessment surveys show an increase in student ability to link Mendelian concepts to experimental procedures relying on DNA analysis. The development of modules such as these provides students of all backgrounds with the tools to engage the complexities and issues that constitute modern principles of inheritance. V C 2013 by The International Union of Biochemistry and Molecular Biology, 41(3): [163][164][165][166][167][168][169][170][171][172] 2013 Keywords: Undergraduate, high school, genetics, module, inquirybased learning A thorough understanding of the principles of inheritance has been a critical part of a complete biological education for as long as mankind has studied biology. The advances in molecular biology in the past few decades brought on by genetic engineering and the -omics revolutions, however, have made the teaching of inheritance a more difficult proposition than in the past. Science's more complete understanding of genetics comes with challenges for science education: How can a teacher adequately teach the principles of Mendelian genetics while at the same time linking those principles to the molecular phenomena that make them possible? To meet the pedagogical challenge of providing a complete picture of genetics for students of all educational interests and backgrounds, it is critical to establish an inquiry-based way by which students can come to appreciate both the basic Mendelian principles of genetics and the ways that molecular biology has better informed these principles.Traditional Mendelian techniques for the teaching of genetics provide a strong logical foundation for the exploration of inheritance but do not address the complexities that underlie the majority of genotype/phenotype interactions [1]. In middle and high school, students traditionally begin their studies of inheritance with Mendel's pea plants and the use of Punnett squares. This is followed later on by a look at DNA and t...