A Leaf Curl Disease in Germplasm of Rapeseed‐Mustard in India: Molecular Evidence of a Weed‐Infecting Begomovirus–Betasatellite Complex Emerging in a New Crop

Abstract

Evaluation of 130 accessions of rapeseed-mustard germplasm grown at the National Bureau of Plant Genetic Resources, New Delhi, India during the winter season (2011)(2012) revealed the occurrence of a leaf curl disease in seven accessions. The occurrence of the disease was observed in another 62 of 525 accessions evaluated during 2012-2013. The association of a monopartite begomovirus and betasatellite was established with the symptomatic plants by whitefly transmission and PCR amplification. The complete nucleotide sequences of the begomovirus (JX270684, 2745 nucleotides), obtained by rolling circle amplification, showed the highest sequence identity (98.1%) with the weed-infecting begomovirus, Croton yellow vein mosaic virus. Analysis of recombination indicated the probable occurrence of many overlapping inter-and intraspecific recombination events. The sequence of betasatellite (JX270685, 1355 nucleotides) showed the highest sequence identity (95.7%) with Croton yellow vein mosaic betasatellite. Begomoviruses were not previously known to naturally infect rapeseed-mustard. This is the first report of the emergence of a weed-infecting begomovirus-betasatellite complex in rapeseed-mustard germplasm in India and raises the concern on utilization of such susceptible germplasm in crop improvement programmes.

Agroinfection of tobacco by croton yellow vein mosaic virus and designing of a replicon vector for expression of foreign gene in plant

“…JX270684) in the pUC18 vector at BamHI site [28]. A partial tandem repeats (PTR) construct was developed from M4A clone in binary vector pCAMBIA 2300 (Fig.…”

“…JX270685) was earlier cloned (M4b) [28]. The genome of CroYVMB was amplified with a mutated forward primer b-01* (5 0 tgtaccactacgctacgag3 0 , T in place of G in restriction site region of KpnI of the universal b-01 primer) and b-02 universal primer [2] using the M4b clone as a template.…”

“…CYVMV is transmitted by whitefly, Bemisia tabaci, and occurs commonly on Croton bonplandianum causing bright yellow vein mosaic disease. Other than C. Bonplandianum, natural infection of CYVMV has been identified in several weeds and economic plant species e.g., Jatropha sp., Acalypha sp., Cyamopsis sp., Crotalaria juncea, okra, papaya, radish, rapeseed-mustard and tomato [15,27,28,31]. Under the experimental conditions, CYVMV infects as many as 35 plant species of 11 families [27].…”

Identification and Functional Analysis of Four RNA Silencing Suppressors in Begomovirus Croton Yellow Vein Mosaic Virus

“…Croton yellow vein mosaic virus (CYVMV), a species in the genus Begomovirus, is a prolific begomovirus in the Indian subcontinent. CYVMV was first reported from the weed host Croton sparsiflorus and later found on other crops such as Jatropha gossypifolia (Snehi et al, 2011), cluster bean (Zaffalon et al, 2012), radish (Singh et al, 2012), rapeseed-mustard (Roy et al, 2013), and Crambe (Kumar et al, 2018). Under the experimental conditions, a total of 35 plant species from 11 families have been reported as hosts of CYVMV (Pramesh et al, 2013).…”

Genomic characterization of recombinant cucumber mosaic virus isolates infecting mustard species via HTS in India

“…trilocularis ), thought to have Mediterranean origin; brown mustard, sometimes known as Indian mustard ( Brassica juncea ); and black mustard ( B. nigra ), thought to have Asia Minor‐Iran origin. Several viruses have been found to infect mustard in India, including turnip yellows virus (TuYV) (Sharma et al, 2013), turnip mosaic virus (TuMV) (Singhal et al, 2022), croton yellow vein mosaic virus (CYVMV) (Roy et al, 2013).…”