A lateral flow immunosensor for direct, sensitive, and highly selective detection of hemoglobin A1c in whole blood

Ang, Shu Hwang; Thevarajah, T. Malathi; Woi, Pei Meng; Alias, Yatimah; Khor, Sook Mei

doi:10.1016/j.jchromb.2016.01.059

Cited by 15 publications

(2 citation statements)

References 21 publications

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“…Methods of HbA1c measurement classified into two groups techniques based on structural differences and based on chemical differences; a) Separation based on charge difference (ion-exchange chromatography [61,128], electrophoresis [61,78] and isoelectric focusing), b) Separation based on structural characteristics of glycogroups on haemoglobin (immunoassay [10,118] and affinity chromatography [66,101,132]), and, c) Separation based on chemical reactivity (photometry, electrospray mass spectrometry)(See Fig. 2).…”

Section: Methods For Measurement Of Hba1c In Samples (Diabetic Patients)mentioning

confidence: 99%

Standard of Care in Diagnosis of Diabetes Mellitus: HbA1c

2023

MCMS

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Objective: The HbA1c concentration is a crucial component of routine diabetes therapy since it serves both as a risk predictor and an indicator of long-term glycemia and represents the average glycemic history of the previous two to three months.The HbA1c assay was approved as the preferred approach for diagnosing diabetes by the American Diabetes Association, the European Association for the Study of Diabetes, and the International Diabetes Federation. This article provides an overview of HbA1c to comprehend its importance in the routine monitoring of diabetic patients. Topics discussed include measurement techniques, advantages and disadvantages of different measurement techniques, clinical significance of HbA1c, rapid diagnosis of HbA1c, and alternative means available to assess glycaemic control.Methods: This review highlights in detail the structure and formation of HbA1c, clinical importance factors influencing HbA1c levels, methods of measurement, the challenges associated with measuring the HbA1c concentration and describes the current state of the art of analytical and clinical aspects of the process.Results: Recent developments have made HbA1c assays more sensitive, simple to use, and less expensive. In comparison to previous technologies, the point-of-care HbA1c testing devices deliver quantifiable findings while limiting inference of other Hb variations, making diagnosis far more reliable. Conclusion:A person with diabetes can live a long and high -quality life if regular monitoring and treatment of diabetes should be done properly. This review emphasizes the shortcomings faced by recent diagnostics techniques and the correlation between falsely elevated and lowered HbA1c results with different physical conditions.

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Section: Methods For Measurement Of Hba1c In Samples (Diabetic Patients)mentioning

confidence: 99%

Standard of Care in Diagnosis of Diabetes Mellitus: HbA1c

2023

MCMS

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“…This includes blood containing anticoagulants, blood that has been diluted beforehand with a running buffer, or blood that has been separated from certain components, such as red blood cells, to use only the remaining plasma. [10][11][12][13] These intricate processing steps pose a significant barrier to regular, lowcost health monitoring based on blood biomarkers. While a major focus of the research community lies in investigating pain-free blood sampling and devices for venous blood analysis, menstrual blood appears to be a largely ignored sampling source.…”

Section: Introductionmentioning

confidence: 99%

A Wearable In-pad Diagnostic for the Detection of Disease Biomarkers in Menstruation Blood

Dosnon,

Rduch,

Meyer

et al. 2024

Preprint

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The pain-free regular monitoring of blood-based biomarkers is a highly appealing yet difficult-to-realize approach for the early detection of pathological changes, including cancers, infections, or metabolic diseases, such as diabetes. While a major focus of the research community lies on the investigation of pain-free blood sampling and devices for venous blood analysis, menstruation blood remains a largely ignored sampling source. Growing evidence shows excellent correlation between biomarker levels in menstruation blood and venous blood for an entire clinical panel of analytes. Here, we introduce a wearable, microfluidic diagnostic platform integrated into standard hygiene pads for the electronic-free naked eye-readable direct detection of disease biomarkers in menstruation blood (MenstruAI). We demonstrate semi-quantitative biomarker detection from menstruation using infection and inflammation biomarker C-reactive protein (CRP), gynecological cancer biomarkers (CEA and CA-125), and endometriosis biomarker CA-125 as representative examples of relevant proteinaceous biomarkers. The color-changes induced by the presence of these biomarkers can be read-out by the naked eye as well as by a machine-learning algorithm implemented into a smartphone-app, enabling semi-quantitative analysis. The presented MenstruAI platform has the potential to revolutionize women's health by providing a non-invasive, affordable, and accessible approach to health monitoring, potentially democratizing healthcare by making health services more available and equitable.

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One‐step Electrodeposition of Tris(hydroxymethyl) Aminomethane – Prussian Blue on Screen‐printed Electrode for Highly Efficient Detection of Glycosylated Hemoglobin

Shi

Teng

et al. 2018

Electroanalysis

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In this work, the modified Prussian blue (PB) film showed more stable performance in alkaline solution by one‐step electrodepositon of PB with tris(hydroxymethyl) aminomethane (Tris) on screen‐printed electrode (SPE). The morphology and structure of the modified Tris‐PB/SPE was characterized by scanning electronic microscopy, infra spectroscopy, Raman spectroscopy and X‐ray diffraction. It was inferred that the Tris particles embedded in the PB deposit layer resulted in the change of PB structure and improve its stability in alkaline solution. And then, the modified Tris‐PB/SPE was applied in the detection of Glycosylated hemoglobin (HbA1c). The optimum experimental conditions are pH 7.5, 100 mV/s, 4 μL FAOD and 5 min reaction time. The linearship of HbA1c is i=22.90 C+101.9 in the range of 0.1–2 mmol/L. Comparing with PB/SPE, Tris‐PB/SPE shows better sensitivity and recovery.

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A lateral flow immunosensor for direct, sensitive, and highly selective detection of hemoglobin A1c in whole blood

Cited by 15 publications

References 21 publications

Standard of Care in Diagnosis of Diabetes Mellitus: HbA1c

Standard of Care in Diagnosis of Diabetes Mellitus: HbA1c

A Wearable In-pad Diagnostic for the Detection of Disease Biomarkers in Menstruation Blood

One‐step Electrodeposition of Tris(hydroxymethyl) Aminomethane – Prussian Blue on Screen‐printed Electrode for Highly Efficient Detection of Glycosylated Hemoglobin

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