A lateral flow immunoassay for measuring ochratoxin A: Development of a single system for maize, wheat and durum wheat

Lateral flow assays: Principles, designs and labels

“…Another advantage of BSA is that it avoids the matrix interference. Apart from BSA, other proteins, surfactants, polymers, and organic cations have been used as AuNPs stabilization agents in the labelled antibody solution and as a modifier of the nitrocellulose membrane [31].…”

“…It is a small molecule and applicable for competitive LFA analysis. A LFA based on aptamer [28] and antibody [29,30,31,32] was used for OTA detection. In literature, different detection limits were found, such as 0.18 ng/mL in aptamer based study [28] and 0.5 ng/mL in antibody-based study [29].…”

“…The type of nitrocellulose membrane affects the detection time. Laura et al [31] and Majdinasab et al [30] used HiFlow 80 and Millipore NC membrane and the detection limits were 1.5 ng/mL and 0.25 ng/mL.…”

Rapid and sensitive quantitation of zearalenone in food and feed by lateral flow immunoassay

“…In addition, the expenses of staff training and analysis time are highly reduced compared to other techniques. In the past few years, LFA was used for the detection of mycotoxins such as aflatoxin B 2 (Tang et al, 2009), ochratoxin A (Lai, Fung, Yang, Renrong, & Xiong, 2009;Laura, Gilda, Claudio, Cristina, & Gianfranco, 2011), deoxynivalenol (Kolosova et al, 2008;Xu et al, 2010), and fumonisin B 1 , B 2 and B 3 (Li et al, 2012). An LFA format for ZEN detection was also developed (Kolosova, De Saeger, Sibanda, Verheijen, & Van Peteghem, 2007).…”

Lateral flow based immunobiosensors for detection of food contaminants

“…Furthermore, saxitoxin, brevitoxin, okadaic acid and domoic acid as phycotoxin in different samples have been analyzed by lateral flow test which is summarized in Table 2. (Liu, Tsao et al 2008) A quantitative lateral flow immunoassay has been developed by Laura et.al (Laura, Gilda et al 2011) for detection of ochratoxin A (OTA) with the detection limit of 1.5 μg kg −1 with recoveries from 87 to 119%. Nitrocellulose membrane has been used in the device which capturing reagents immobilized on that, and a conjugate pad has also been applied to which the antibody labeled with gold nanoparticles was pre-adsorbed.…”