A lateral flow biosensor for detection of single nucleotide polymorphism by circular strand displacement reaction

Xiao, Zhuo; Lie, Puchang; Fang, Zhiyuan; Yu, Luxin; Chen, Junhua; Liu, Jie; Ge, Chenchen; Zhou, Xuemeng; Zeng, Lingwen

doi:10.1039/c2cc33931e

Cited by 26 publications

(16 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The limit of detection (LOD) of the assay was 0.01 fM and the quantitative analysis was based on the readout of optical density of AuNPs at the test line using a portable strip reader. Xiao et al reported a lateral flow nucleic acid bioassay that made use of AuNPs as labels for visual detection and circular strand displacement polymerase reaction for target amplification [25]. The LOD of the assay was 0.01 fM and the assay was suitable for the detection of human genomic DNA from blood samples.…”

Section: Introductionmentioning

confidence: 97%

Ratiometric fluorescence transduction by hybridization after isothermal amplification for determination of zeptomole quantities of oligonucleotide biomarkers with a paper-based platform and camera-based detection

Noor

Hrovat

Moazami-Goudarzi

et al. 2015

Analytica Chimica Acta

View full text Add to dashboard Cite

Paper is a promising platform for the development of decentralized diagnostic assays owing to the low cost and ease of use of paper-based analytical devices (PADs). It can be challenging to detect on PADs very low concentrations of nucleic acid biomarkers of lengths as used in clinical assays. Herein we report the use of thermophilic helicase-dependent amplification (tHDA) in combination with a paper-based platform for fluorescence detection of probe-target hybridization. Paper substrates were patterned using wax printing. The cellulosic fibers were chemically derivatized with imidazole groups for the assembly of the transduction interface that consisted of immobilized quantum dot (QD)-probe oligonucleotide conjugates. Green-emitting QDs (gQDs) served as donors with Cy3 as the acceptor dye in a fluorescence resonance energy transfer (FRET)-based transduction method. After probe-target hybridization, a further hybridization event with a reporter sequence brought the Cy3 acceptor dye in close proximity to the surface of immobilized gQDs, triggering a FRET sensitized emission that served as an analytical signal. Ratiometric detection was evaluated using both an epifluorescence microscope and a low-cost iPad camera as detectors. Addition of the tHDA method for target amplification to produce sequences of ∼100 base length allowed for the detection of zmol quantities of nucleic acid targets using the two detection platforms. The ratiometric QD-FRET transduction method not only offered improved assay precision, but also lowered the limit of detection of the assay when compared with the non-ratiometric QD-FRET transduction method. The selectivity of the hybridization assays was demonstrated by the detection of single nucleotide polymorphism.

show abstract

Section: Introductionmentioning

confidence: 97%

Ratiometric fluorescence transduction by hybridization after isothermal amplification for determination of zeptomole quantities of oligonucleotide biomarkers with a paper-based platform and camera-based detection

Noor

Hrovat

Moazami-Goudarzi

et al. 2015

Analytica Chimica Acta

View full text Add to dashboard Cite

show abstract

“…Meanwhile, in order to solve the specificity problem associated with a single hairpin probe, it is feasible to design multiple specific hairpin probes for a target with parallel processing. For the detection of a single nucleotide polymorphism, the combination of using high-fidelity T4 DNA ligase with CSDPR has shown good potential in personalized medicine; for example in the detection of cancerrelated mutations and drug-resistant mutations of infectious agents [7].…”

Section: Specificity and Sensitivitymentioning

confidence: 99%

Circular Strand Displacement Polymerization Reaction: A Promising Technique?

Wang

Zeng

2014

Bioanalysis

Self Cite

View full text Add to dashboard Cite

“…As a result, AuNPs have been extensively explored for biosensing [66], imaging [67], and photothermal cancer therapy [68]. The ability to pegylate AuNPs allows them to preferentially accumulate in tumors via the EPR effect [69].…”

Section: Synthesis Of Hybrid Nanomaterialsmentioning

confidence: 99%

Hybrid nanoparticles for combination therapy of cancer

Lin

2015

Journal of Controlled Release

122

View full text Add to dashboard Cite

Nanoparticle anticancer drug delivery enhances therapeutic efficacies and reduces side effects by improving pharmacokinetics and biodistributions of the drug payloads in animal models. Despite promising preclinical efficacy results, monotherapy nanomedicines have failed to produce enhanced response rates over conventional chemotherapy in human clinical trials. The discrepancy between preclinical data and clinical outcomes is believed to result from the less pronounced enhanced permeability and retention (EPR) effect in and the heterogeneity of human tumors as well as the intrinsic/acquired drug resistance to monotherapy over the treatment course. To address these issues, recent efforts have been devoted to developing nanocarriers that can efficiently deliver multiple therapeutics with controlled release properties and increased tumor deposition. In ideal scenarios, the drug or therapeutic modality combinations have different mechanisms of action to afford synergistic effects. In this review, we summarize recent progress in designing hybrid nanoparticles for the co-delivery of combination therapies, including multiple chemotherapeutics, chemotherapeutics and biologics, chemotherapeutics and photodynamic therapy, and chemotherapeutics and radiotherapy. The in vitro and in vivo anticancer effects are also discussed.

show abstract

A lateral flow biosensor for detection of single nucleotide polymorphism by circular strand displacement reaction

Cited by 26 publications

References 17 publications

Ratiometric fluorescence transduction by hybridization after isothermal amplification for determination of zeptomole quantities of oligonucleotide biomarkers with a paper-based platform and camera-based detection

Ratiometric fluorescence transduction by hybridization after isothermal amplification for determination of zeptomole quantities of oligonucleotide biomarkers with a paper-based platform and camera-based detection

Circular Strand Displacement Polymerization Reaction: A Promising Technique?

Hybrid nanoparticles for combination therapy of cancer

Contact Info

Product

Resources

About