A Late Effect of Radiation on the Haemopoietic Stem Cells of the Mouse

Covelli, Vincenzo; Metalli, P.

doi:10.1080/09553007314550081

Cited by 17 publications

(5 citation statements)

References 14 publications

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“…Our results for adult mice irradiated with a single dose of 7 Gy reflect a high depression of CFU-S in the marrow at the times studied, consistent with others previously reported (Covelli and Metalli 1973) . Here we found that the committed CFU-GM and BFU-E were equally depleted to 30% of control, from the sixth to the fifteenth month postirradiation ( Figure 1) and only some sign of recovery was noted (60% of control) at the latest time analysed (20 months) .…”

Section: Discussionsupporting

confidence: 93%

Residual Haematopoietic Damage in Adult and 8 Day-old Mice Exposed to 7 Gy of X-rays

Grande¹,

Gaitán²,

Tejero³

et al. 1993

International Journal of Radiation Biology

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Our experiments have focused on the analysis of residual haematopoietic damage in 8-day-old and 12-week-old mice X-irradiated with a single dose of 7 Gy. In the case of the adult mice, analysis of the femoral and splenic CFU-S, CFU-GM and BFU-E showed a persistent depletion of these haematopoietic progenitor cells after irradiation. In contrast, in 1-week-old irradiated mice, a progressive recovery of the femoral haematopoietic progenitors was observed, achieving essentially normal values 1 year after irradiation. The spleens of these mice, however, contained significantly less haematopoietic progenitors than the control group, mainly as a consequence of the size reduction of this organ. In the peripheral blood, normal cellularity values were observed in most cases, although in the adult group a decline in numbers or circulating cells was noted after the first year following irradiation. Long-term bone marrow cultures (LTBMCs) established 1 year after the irradiation of adult mice generated poor adherent layers, while confluent stromas were observed in flasks corresponding to 8-day-old irradiated mice. Analyses of the functional activity of granulocytes obtained from either the peripheral blood or LTBMCs revealed that both young and adult irradiated mice produced granulocytes generating high levels of superoxide anion, in relation to age-matched controls. This finding was correlated with a long-term microenvironmental activation which resulted in an overproduction of granulocyte-macrophage colony-stimulating factor.

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Section: Discussionsupporting

confidence: 93%

Residual Haematopoietic Damage in Adult and 8 Day-old Mice Exposed to 7 Gy of X-rays

Grande¹,

Gaitán²,

Tejero³

et al. 1993

International Journal of Radiation Biology

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“…These results are consistent with the dose-dependent increase in CFU-S proliferation observed after gamma-irradiation (Tejero et al . 1988) and with those reports that document impaired recovery of CFU-S as a late effect of sublethal X-irradiation (Carsten and Bond 1969, Covelli and Metalli 1973, Croizat et al . 1971, Xu et al .…”

Section: Discussionmentioning

confidence: 71%

Late Effects of X-irradiation on Haemopoietic Stem Cells in CBA/H Mice

Lorimore

Wright

1990

International Journal of Radiation Biology

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CBA/H male mice have been used to study the recovery of haemopoietic stem cells following 3 Gy whole-body X-irradiation. Despite significant proliferation haemopoietic stem cells (measured as spleen colony-forming units or CFU-S) did not recover to control values and their numbers remained sub-optimal at 600 days post-irradiation. At this time there were significant increases in both proliferation and self-renewal characteristics of the CFU-S but no impairment of the capacity of bone marrow cells to rescue and repopulate lethally irradiated transplant recipients.

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“…In experiments with mice, rather controversial data on the changes of femoral CFU and CFU-ratio as compared in young (i.e. in average 2-3 months of age) and old (from 20 months to over 2 years of age) mice were reported: an increase was observed (Yuhas & Storer, 1967;Metcalf & Moore, 1971) as well as no changes (Kretchmar & Conover, 1967;Davis et al, 1971) or even a decrease (Chen, 1971;Davis et al, 1971;Covelli & Metalli, 1973;Silini & Andreozzi, 1974). In the spleen a modest fall in the total CFU content and the CFU-ratio has been reported (Albright & Makinodan, 1966;Chen, 1971 ;Mini & Andreozzi, 1974) as well as no differences in the CFU-ratio and slight reduction of total CFU content in the spleen of ageing mice (Metcalf & Moore, 1971).…”

Section: (B) Influence Of the Donor's Agementioning

confidence: 99%

Age Dependence of the Number of the Stem Cells in Haemopoietic Tissues of Rats

1976

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The number and concentration of haemopoietic stem cells in the femoral bone marrow and spleen of Wistar rats of different ages were investigated. Stem cells were assayed by the spleen colony technique in irradiated rat recipients. The ability of the recipient spleen to harvest transplanted tissue as a macroscopic colony was found to be dependent on the recipient's age. Changes with senescence were observed also in the concentration and the size of the stem cell compartment both in the marrow and spleen. No differences were demonstrated in the seeding of transplanted colony‐forming units into the spleen of recipients of 1 and 4 months of age. A rats‐mice strain difference in the effect of senescence on the haemopoietic stem cells is discussed.

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A Late Effect of Radiation on the Haemopoietic Stem Cells of the Mouse

Cited by 17 publications

References 14 publications

Residual Haematopoietic Damage in Adult and 8 Day-old Mice Exposed to 7 Gy of X-rays

Residual Haematopoietic Damage in Adult and 8 Day-old Mice Exposed to 7 Gy of X-rays

Late Effects of X-irradiation on Haemopoietic Stem Cells in CBA/H Mice

Age Dependence of the Number of the Stem Cells in Haemopoietic Tissues of Rats

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