A laser light scattering in situ system for counting aggregates in blood platelet aggregation

Abstract

A novel system has been developed to evaluate biochemically induced blood platelet aggregation by means of a particle counting technique that uses light scattering. Variations in the particle size and concentration were measured by detecting the scattered light intensity and the number of particles, respectively, passing through a laser beam. The system is capable of performing sensitive, in situ continuous measurements of platelet aggregation by counting and sizing of aggregates.-

Biocompatible micellar nanovectors achieve efficient gene transfer to vascular lesions without cytotoxicity and thrombus formation

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Platelet aggregates in various stages of diabetic retinopathy: evaluation using the particle-counting light-scattering method

“…This method is more sensitive than the optical-density method for detecting small aggregate formations [16,17,23,27]. The light-scattering method can also quantify spontaneous platelet aggregates that form in the absence of agonist stimulation.…”

Novel endotoxin assay by laser light‐scattering particle‐counting method

“…The 200 ml of test solution in the glass cuvette was kept at 371C and stirred continuously at 1,000 rpm (18,19). The signal count represented the number of particles and the scattered light intensity represented particle size.…”