“…In addition, we evaluated the expression of genes specific to each cluster (Moran's I test, q-value < 0.05) in a laser capture microdissection (LCM, Schnabel et al, 2023) dataset that investigated the response of M. truncatula to rhizobia infection at similar time points as the ones used in the present study (see Methods; Figures S2-S7). Based on the annotation approach described above, we identified nuclei derived from six types of root cells: the epidermal cells (clusters 1, 3, 14, and 22), root hair (clusters 12), cortex (clusters 2, 4, 9, 11, 13, and 16), endodermis (clusters 7 and 24), pericycle (cluster 8) and stele (excluding pericycle; clusters 10, 18, 23 and 26) (Figure 1B-C).…”