A large‐scale study of Yap1p‐dependent genes in normal aerobic and H₂O₂‐stress conditions: the role of Yap1p in cell proliferation control in yeast

Abstract

SummaryYeast genes regulated by the transcriptional activator Yap1p were screened by two independent methods: (i) use of a LacZ-fused gene library and (ii) highdensity membrane hybridization. Changes in transcriptome profile were examined in the presence and in the absence of Yap1p, as well as under normal and H 2 O 2 -mediated stress conditions. Both approaches gave coherent results, leading to the identification of many genes that appear to be directly or indirectly regulated by Yap1p. Promoter sequence analysis of target genes revealed that this regulatory effect is not always dependent upon the presence of a Yap1p binding site. The results show that the regulatory role of Yap1p is not restricted to the activation of stress response but that this factor can act as a positive or a negative regulator, both under normal and oxidative stress conditions. Among the targets, a few genes participating in growth control cascades were detected. In particular, the RPI1 gene, a repressor of the ras-cAMP pathway, was found to be downregulated by Yap1p during the early phase of growth, but upregulated in the stationary phase or after oxidative stress.

A systems approach to delineate functions of paralogous transcription factors: Role of the Yap family in the DNA damage response

“…Studies suggest that Yaps 2 and 6 can function either as activators or repressors, depending on the regulated genes (6,29,30). To further explore this phenomenon and its underlying mechanisms, we applied network component analysis (NCA) (31) to infer the regulatory directions (activating, repressing) of each Yap on its target genes.…”

Disruption of Yeast Forkhead-associated Cell Cycle Transcription by Oxidative Stress

“…Oxidative stress in yeast has been studied by exposing cells to agents that are already reactive, typically HP, or drugs that cause the intracellular accumulation of reactive oxygen species (Godon et al, 1998;Dumond et al, 2000;Gasch et al, 2000). Menadione (MD) is such a drug, generating reactive superoxide ions, which can be further oxidized to give HP (Monks et al, 1992;Shackelford et al, 2000).…”

“…They pose a threat to organisms by damaging a variety of cellular macromolecules, including DNA, membrane lipids, and proteins and are implicated with carcinogenesis, aging, and numerous degenerative diseases (Finkel and Holbrook, 2000;Neumann et al, 2003). The major ROS derived from oxygen are superoxide ions, hydrogen peroxide (HP), and hydroxy radicals, ordered here by increasing reactivity (reviewed in Shackelford et al, 2000).Oxidative stress in yeast has been studied by exposing cells to agents that are already reactive, typically HP, or drugs that cause the intracellular accumulation of reactive oxygen species (Godon et al, 1998;Dumond et al, 2000;Gasch et al, 2000). Menadione (MD) is such a drug, generating reactive superoxide ions, which can be further oxidized to give HP (Monks et al, 1992;Shackelford et al, 2000).…”

YAP4 gene expression is induced in response to several forms of stress in Saccharomyces cerevisiae

“…Several studies have demonstrated that Yap1p, a b-Zip protein belonging to the yeast Yap family of transcriptional regulators (Rodrigues-Pousada et al, 2004), plays a central role in sensing and modulating gene expression in response to several forms of oxidative stress (Alarco et al, 1997;Delaunay et al, 2000;Gasch et al, 2000;Lee et al, 1999;Dumond et al, 2000;Nguyen et al, 2001;Wemmie et al, 1994;1366 T. Nevitt, J. Pereira and C. Rodrigues-Pousada Azevedo et al, 2003). Yap1p-responsive genes typically contain within their promoter region a Yap-response element (YRE-TTAC/GTAA) encoding for most cellular proteins with antioxidant function and several membrane-associated drug efflux pumps .…”