A landscape of differentiated biological processes involved in the initiation of sex differentiation in zebrafish

Ye, Ding; Tu, Yi‐Xuan; Wang, Houpeng; He, Mudan; Wang, Yaqing; Chen, Zhengfang; Chen, Zhenxia; Sun, Yuhan

doi:10.1101/2022.04.14.488425

Cited by 2 publications

(1 citation statement)

References 75 publications

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“…In the application of surrogate reproduction by spermatogonial transplantation, only undifferentiated SPG-A, also known as spermatogonial stem cells, could be efficiently differentiate into mature gametes in the host fish (Iwasaki-Takahashi et al, 2020; Wang et al, 2022; Xie et al, 2020). Therefore, evaluation of spermatogenetic status of a fish by accurate identifying and quantifying different types of spermatogenic cells, is a very important work not only for fish reproductive research (Ye et al, 2022; Zhang, Q. et al, 2020), but also for germ cell-based genetic breeding such as surrogate reproduction (Jin et al, 2021; Zhang et al, 2022; Zhang, F. et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Assessment of fish spermatogenesis through high-quality immunofluorescence against fish testicular samples with an antibody set

Liu

et al. 2022

Preprint

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A complete evaluation of the spermatogenetic status of a fish by accurately identifying different types of spermatogenic cells is useful not only for reproductive studies but also for genetic breeding. For this task, it is required to establish a simple and practical experimental procedure, to obtain repeatable, high-quality imaging data. Here, we have developed antibodies against the zebrafish (Danio rerio) spermatogenesis-related proteins, including Ddx4, Piwil1, Sycp3, and Pcna, and an integrated method for high-quality and high-through output immunofluorescence on testis sections of different fish species. We accurately identified different spermatogenic cells at different stages. Type-A spermatogonia can be identified by the highest expression of Ddx4 and Piwil1 among different spermatogenic cells. Type-B spermatogonia is identified by the second highest expression of Ddx4 and the highest expression of Pcna among different spermatogenic cells. Spermatids can be distinguished from spermatozoa by the expression of Piwil1. The different subtypes of primary spermatocytes (SPC-I) can be identified by co-staining of Sycp3 and Pcna. Leptotene SPC-I show polar expression of both Sycp3 and Pcna at the same side of the nucleus. All the antibodies were tested for practicality in four fish species, Chinese rare minnow (Gobiocypris rarus), common carp (Cyprinus carpio), blunt snout bream (Megalobrama amblycephala), and rice field eel (Monopterus albus). Using this method and the antibody sets, we were able to precisely and accurately evaluate the spermatogenetic status in different fish species.

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Section: Introductionmentioning

confidence: 99%

Assessment of fish spermatogenesis through high-quality immunofluorescence against fish testicular samples with an antibody set

Liu

et al. 2022

Preprint

Self Cite

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Exploring the Effects of Rearing Densities on Epigenetic Modifications in the Zebrafish Gonads

Valdivieso,

Caballero-Huertas,

Moraleda-Prados

et al. 2023

IJMS

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Rearing density directly impacts fish welfare, which, in turn, affects productivity in aquaculture. Previous studies have indicated that high-density rearing during sexual development in fish can induce stress, resulting in a tendency towards male-biased sex ratios in the populations. In recent years, research has defined the relevance of the interactions between the environment and epigenetics playing a key role in the final phenotype. However, the underlying epigenetic mechanisms of individuals exposed to confinement remain elucidated. By using zebrafish (Danio rerio), the DNA methylation promotor region and the gene expression patterns of six genes, namely dnmt1, cyp19a1a, dmrt1, cyp11c1, hsd17b1, and hsd11b2, involved in the DNA maintenance methylation, reproduction, and stress were assessed. Zebrafish larvae were subjected to two high-density conditions (9 and 66 fish/L) during two periods of overlapping sex differentiation of this species (7 to 18 and 18 to 45 days post-fertilization, dpf). Results showed a significant masculinization in the populations of fish subjected to high densities from 18 to 45 dpf. In adulthood, the dnmt1 gene was differentially hypomethylated in ovaries and its expression was significantly downregulated in the testes of fish exposed to high-density. Further, the cyp19a1a gene showed downregulation of gene expression in the ovaries of fish subjected to elevated density, as previously observed in other studies. We proposed dnmt1 as a potential testicular epimarker and the expression of ovarian cyp19a1a as a potential biomarker for predicting stress originated from high densities during the early stages of development. These findings highlight the importance of rearing densities by long-lasting effects in adulthood conveying cautions for stocking protocols in fish hatcheries.

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A landscape of differentiated biological processes involved in the initiation of sex differentiation in zebrafish

Cited by 2 publications

References 75 publications

Assessment of fish spermatogenesis through high-quality immunofluorescence against fish testicular samples with an antibody set

Assessment of fish spermatogenesis through high-quality immunofluorescence against fish testicular samples with an antibody set

Exploring the Effects of Rearing Densities on Epigenetic Modifications in the Zebrafish Gonads

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