A laminar flow expansion chamber facilitating downstream manipulation of particles concentrated using an ultrasonic standing wave

Hawkes, Jeremy J.; Barrow, David A.; Cefai, Joseph J.; Coakley, W. Terence

doi:10.1016/s0041-624x(98)00018-3

Cited by 30 publications

(9 citation statements)

References 10 publications

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“…The ultrasonic process, like many other nascent technologies, is extremely amenable to automation and it is possible to envisage an entirely selfcontained¯ow-through system [22] using photometric analysis to enable rapid specimen turnover. Photometric methods are easily incorporated into on-line systems [55,56] and turbidimetry has been used in the ultrasonic detection of puri®ed rotavirus outer coat antigen with commercial LPA reagents [33].…”

Section: Potential For Automated Multiple Sample Analysismentioning

confidence: 99%

Diagnostic particle agglutination using ultrasound: a new technology to rejuvenate old microbiological methods

Ellis¹,

Sobański

2000

Journal of Medical Microbiology

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Section: Potential For Automated Multiple Sample Analysismentioning

confidence: 99%

Diagnostic particle agglutination using ultrasound: a new technology to rejuvenate old microbiological methods

Ellis¹,

Sobański

2000

Journal of Medical Microbiology

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“…During USWF exposure, microparticles (i.e., suspended cells) are driven to the pressure nodal planes by the primary radiation force, which then aggregate to form cell bands separated by half-wavelength intervals (Whitworth and Coakley 1992). Theoretically, suspension cells can arrive at the pressure nodal planes in a time scale of seconds, while the nanoparticles (i.e., nonviral DNA vectors) proceed over a time scale of minutes (Hawkes et al 1998). However, nanometer-sized DNA vectors were not standstill on the pressure nodal planes instead circulating between pressure nodal planes because microstreaming-induced drag force (f) is comparable to the primary radiation force.…”

Section: Introductionmentioning

confidence: 99%

Nonviral Transfection of Suspension Cells in Ultrasound Standing Wave Fields

Lee¹,

Peng²

2007

Ultrasound in Medicine & Biology

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Ultrasound-induced cavitation has been widely used for delivering DNA vectors into cells. However, this approach may seriously disrupt cell membranes and cause lethal damage when cells are exposed to the inertial cavitation field. In this study, instead of using sonoporation, ultrasound standing wave fields (USWF) were explored for nonviral transfection of suspension cells. Acoustic resonance in a tubular chamber was generated from the interference of waves emitted from a piezoelectric transducer and consequently reflected from a borosilicate glass coverslip. The suspended K562 erythroleukemia cells were transfected by polyethyleneimine (PEI)/DNA complexes with and without exposure to 1-MHz USWF for 5 min. During USWF exposure, K562 cells moved to the pressure nodal planes first and formed cell bands by the primary radiation force. Nanometer-sized PEI/DNA complexes, circulated between nodal planes by acoustic microstreaming, then used the cell agglomerates as the nucleating sites on which to attach. After incubation at 37 degrees C for 48 h, the efficiency of nonviral transfection based on EGFP transgene expression was determined by fluorescent microscopy and fluorometry. Both studies showed that USWF brought suspended K562 cells and PEI/DNA complexes into close contact at the pressure nodal planes, yielding an approximately 10-fold increment of EGFP transgene expression compared with the group without ultrasonic treatment.

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“…The motions of micrometer-sized particles (e.g., mammalian cells) in non-cavitation, MHz-frequency-ranged RUF have been known to be driven by both the primary ( F 1 ) and the secondary ( F 2 ) acoustic radiation forces [ 21 , 22 ] that and where P 0 is the peak pressure amplitude of the ultrasonic standing wave; λ is sound wavelength; V is the volume of the particles; z represents the propagating distance of the ultrasonic wave which is perpendicular to the pressure nodal planes; ρ p and β p are the density and compressibility, respectively, of the particles and ρ 0 and β 0 denote the density and compressibility, respectively, of the surrounding bulk phase; R 0 is the radius of the particle; d is the distance between particles; θ is the angle between the centerline of the particle and the propagating direction of the sound wave; ω is the angular velocity; v denotes the particle velocity; and p is the amplitude of acoustic pressure at the pressure nodes. Theoretically, microparticles can arrive at acoustic pressure nodes within seconds [ 23 ], which suggests that all the cells and drug microcarriers can meet in 3D quickly under RUF exposure. Therefore, we hypothesized that we could utilize the drug-loaded microcarriers and RUF to enhance the efficiency of drug delivery to macrophages by increasing the contact opportunities between cells and drug vehicles as illustrated in Fig 1 .…”

Section: Introductionmentioning

confidence: 99%

Enhancing Macrophage Drug Delivery Efficiency via Co-Localization of Cells and Drug-Loaded Microcarriers in 3D Resonant Ultrasound Field

Lee

2015

PLoS ONE

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In this study, a novel synthetic 3D molecular transfer system which involved the use of model drug calcein-AM-encapsulated poly(lactic-co-glycolic acid) microspheres (CAPMs) and resonant ultrasound field (RUF) with frequency of 1 MHz and output intensity of 0.5 W/cm2 for macrophage drug delivery was explored. We hypothesized that the efficiency of CAPMs-mediated drug delivery aided by RUF can be promoted by increasing the contact opportunities between cells and the micrometer-sized drug carriers due to effects of acoustic radiation forces generated by RUF. Through the fluoromicroscopic and flow cytometric analyses, our results showed that both DH82 macrophages and CAPMs can be quickly brought to acoustic pressure nodes within 20 sec under RUF exposure, and were consequently aggregated throughout the time course. The efficacy of cellular uptake of CAPMs was enhanced with increased RUF exposure time where a 3-fold augmentation (P < 0.05) was obtained after 15 min of RUF exposure. We further demonstrated that the enhanced CAPM delivery efficiency was mainly contributed by the co-localization of cells and CAPMs resulting from the application of the RUF, rather than from sonoporation. In summary, the developed molecular delivery approach provides a feasible means for macrophage drug delivery.

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A laminar flow expansion chamber facilitating downstream manipulation of particles concentrated using an ultrasonic standing wave

Cited by 30 publications

References 10 publications

Diagnostic particle agglutination using ultrasound: a new technology to rejuvenate old microbiological methods

Diagnostic particle agglutination using ultrasound: a new technology to rejuvenate old microbiological methods

Nonviral Transfection of Suspension Cells in Ultrasound Standing Wave Fields

Enhancing Macrophage Drug Delivery Efficiency via Co-Localization of Cells and Drug-Loaded Microcarriers in 3D Resonant Ultrasound Field

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